Gene description for GPC6
Gene name glypican 6
Gene symbol GPC6
Other names/aliases OMIMD1
Species Homo sapiens
 Database cross references - GPC6
ExoCarta ExoCarta_10082
Entrez Gene 10082
HGNC 4454
MIM 604404
UniProt Q9Y625  
 GPC6 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for GPC6
Molecular Function
    heparan sulfate proteoglycan binding GO:0043395 IEA
Biological Process
    glycosaminoglycan catabolic process GO:0006027 TAS
    carbohydrate metabolic process GO:0005975 TAS
    glycosaminoglycan biosynthetic process GO:0006024 TAS
    retinoid metabolic process GO:0001523 TAS
    chondroitin sulfate metabolic process GO:0030204 TAS
    phototransduction, visible light GO:0007603 TAS
    cell migration GO:0016477 IDA
    small molecule metabolic process GO:0044281 TAS
    glycosaminoglycan metabolic process GO:0030203 TAS
Subcellular Localization
    proteinaceous extracellular matrix GO:0005578 IEA
    anchored component of membrane GO:0031225 IEA
    Golgi lumen GO:0005796 TAS
    plasma membrane GO:0005886 TAS
    integral component of plasma membrane GO:0005887 TAS
    extracellular space GO:0005615 IEA
    lysosomal lumen GO:0043202 TAS
    nucleus GO:0005634 IDA
 Experiment description of studies that identified GPC6 in exosomes
1
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GPC6
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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