Experiment description of studies that identified GDF15 in exosomes

1 Experiment ID 207 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DKO-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

2 Experiment ID 208 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name Dks-8 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

3 Experiment ID 209 MISEV standards ✘ Biophysical techniques ✔ TSG101|HSP70|FLOT1 Enriched markers ✔ VDAC Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23161513     Organism Homo sapiens Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS. Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ." Journal name Mol Cell Proteomics Publication year 2012 Sample Colorectal cancer cells Sample name DLD-1 Isolation/purification methods Differential centrifugation Filtration Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

4 Experiment ID 234 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-C3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

5 Experiment ID 237 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocytes Sample name MIHA Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RNA Sequencing

6 Experiment ID 255 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|FLOT1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name G1 Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

7 Experiment ID 256 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|FLOT1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name 501mel Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

8 Experiment ID 258 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|FLOT1|CD81 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25950383     Organism Homo sapiens Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines Authors "Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L" Journal name Pigment Cell Melanoma Res Publication year 2015 Sample Melanoma cells Sample name SKMEL28 Isolation/purification methods Differential centrifugation Unltracentrifugation Sucrose density gradient Flotation density 1.13 - 1.19 g/mL Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

9 Experiment ID 488 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

10 Experiment ID 145 MISEV standards ✔ EM Biophysical techniques ✔ HSP70|HSP90|RAB5|CD10 Enriched markers ✔ HSP90B1 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [QTOF]    PubMed ID 22723089     Organism Homo sapiens Experiment description Prostate cancer cell derived exosomes Authors "Hosseini-Beheshti E, Guns ES." Journal name MCP Publication year 2012 Sample Prostate cancer cells Sample name C4-2 - Rep 3 Isolation/purification methods Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [QTOF]

Protein-protein interactions for GDF15

Protein Interactor ExoCarta ID Identification method PubMed Species 1 LY86   Affinity Capture-MS Homo sapiens 2 YPEL5 51646 Affinity Capture-MS Homo sapiens 3 SETD4 54093 Affinity Capture-MS Homo sapiens 4 HSPA5 3309 Affinity Capture-MS Homo sapiens 5 TMEM106A   Affinity Capture-MS Homo sapiens 6 RMND5B   Affinity Capture-MS Homo sapiens 7 PI15   Affinity Capture-MS Homo sapiens 8 CST5 1473 Affinity Capture-MS Homo sapiens 9 SORL1 6653 Affinity Capture-MS Homo sapiens 10 STAT5A 6776 Affinity Capture-MS Homo sapiens 11 MAEA 10296 Affinity Capture-MS Homo sapiens 12 ERBB2IP 55914 Affinity Capture-MS Homo sapiens 13 CHST8 64377 Affinity Capture-MS Homo sapiens 14 NLGN3   Two-hybrid Homo sapiens 15 PTCH1   Affinity Capture-MS Homo sapiens 16 CFC1   Affinity Capture-MS Homo sapiens 17 PDGFRA 5156 Affinity Capture-MS Homo sapiens 18 ZMYND19   Affinity Capture-MS Homo sapiens 19 PRG2   Affinity Capture-MS Homo sapiens 20 NUPL1 9818 Affinity Capture-MS Homo sapiens 21 MDFI   Two-hybrid Homo sapiens 22 GID4   Affinity Capture-MS Homo sapiens 23 RANBP9 10048 Affinity Capture-MS Homo sapiens 24 CCDC85C   Affinity Capture-MS Homo sapiens 25 OIT3 170392 Affinity Capture-MS Homo sapiens 26 MRPL50 54534 Affinity Capture-MS Homo sapiens 27 SAE1 10055 Affinity Capture-MS Homo sapiens 28 MORC3 23515 Affinity Capture-MS Homo sapiens 29 HOXA1 3198 Two-hybrid Homo sapiens 30 CELA3B   Affinity Capture-MS Homo sapiens 31 IL5RA   Affinity Capture-MS Homo sapiens 32 SCAP   Affinity Capture-MS Homo sapiens 33 Trip11   Affinity Capture-MS Mus musculus 34 GDF15 9518 Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 35 ASS1 445 Affinity Capture-MS Homo sapiens 36 WBP5   Affinity Capture-MS Homo sapiens 37 HTRA2 27429 Affinity Capture-MS Homo sapiens 38 MSTN   Affinity Capture-MS Homo sapiens 39 CBX3 11335 Two-hybrid Homo sapiens 40 GID8 54994 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 41 CRLF1 9244 Affinity Capture-MS Homo sapiens 42 KRTAP12-2   Two-hybrid Homo sapiens 43 Cdc37 12539 Affinity Capture-MS Mus musculus 44 RANBP10 57610 Affinity Capture-MS Homo sapiens 45 C1orf54   Affinity Capture-MS Homo sapiens 46 SDHA 6389 Affinity Capture-MS Homo sapiens 47 RAB3IP   Affinity Capture-MS Homo sapiens 48 C1QBP 708 Affinity Capture-MS Homo sapiens 49 SLURP1   Affinity Capture-MS Homo sapiens 50 GML   Affinity Capture-MS Homo sapiens 51 TMEM102   Affinity Capture-MS Homo sapiens 52 WDR26 80232 Affinity Capture-MS Homo sapiens 53 AR 367 Affinity Capture-MS Homo sapiens 54 MAPK14 1432 Two-hybrid Homo sapiens 55 ARMC8 25852 Affinity Capture-MS Homo sapiens 56 DPEP2   Affinity Capture-MS Homo sapiens 57 MKLN1   Affinity Capture-MS Homo sapiens 58 Smn1 20595 Affinity Capture-MS Mus musculus 59 RMND5A 64795 Affinity Capture-MS Homo sapiens 60 PRG3   Affinity Capture-MS Homo sapiens View the network

Pathways in which GDF15 is involved

No pathways found