Gene description for STAT5A
Gene name signal transducer and activator of transcription 5A
Gene symbol STAT5A
Other names/aliases MGF
STAT5
Species Homo sapiens
 Database cross references - STAT5A
ExoCarta ExoCarta_6776
Vesiclepedia VP_6776
Entrez Gene 6776
HGNC 11366
MIM 601511
UniProt P42229  
 STAT5A identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for STAT5A
Molecular Function
    RNA polymerase II core promoter sequence-specific DNA binding GO:0000979 IEA
    sequence-specific DNA binding transcription factor activity GO:0003700 IEA
    protein binding GO:0005515 IPI
    signal transducer activity GO:0004871 IEA
Biological Process
    valine metabolic process GO:0006573 ISS
    Peyer's patch development GO:0048541 IEA
    luteinization GO:0001553 IEA
    T cell homeostasis GO:0043029 IEA
    negative regulation of erythrocyte differentiation GO:0045647 IEA
    development of secondary male sexual characteristics GO:0046544 IEA
    female pregnancy GO:0007565 IEA
    mammary gland epithelium development GO:0061180 IEA
    lactation GO:0007595 IEA
    positive regulation of inflammatory response GO:0050729 IEA
    positive regulation of activated T cell proliferation GO:0042104 IEA
    succinate metabolic process GO:0006105 ISS
    lipid storage GO:0019915 IEA
    JAK-STAT cascade involved in growth hormone signaling pathway GO:0060397 TAS
    creatinine metabolic process GO:0046449 ISS
    oxaloacetate metabolic process GO:0006107 ISS
    regulation of multicellular organism growth GO:0040014 ISS
    development of secondary female sexual characteristics GO:0046543 IEA
    isoleucine metabolic process GO:0006549 ISS
    2-oxoglutarate metabolic process GO:0006103 ISS
    positive regulation of natural killer cell differentiation GO:0032825 IEA
    positive regulation of natural killer cell mediated cytotoxicity GO:0045954 IEA
    regulation of steroid metabolic process GO:0019218 IEA
    positive regulation of interleukin-2 biosynthetic process GO:0045086 IEA
    fatty acid metabolic process GO:0006631 ISS
    citrate metabolic process GO:0006101 ISS
    negative regulation of mast cell apoptotic process GO:0033026 IEA
    prolactin signaling pathway GO:0038161 ISS
    peptidyl-tyrosine phosphorylation GO:0018108 IEA
    allantoin metabolic process GO:0000255 ISS
    positive regulation of mast cell differentiation GO:0060376 IEA
    creatine metabolic process GO:0006600 ISS
    natural killer cell differentiation GO:0001779 IEA
    positive regulation of mitotic cell cycle GO:0045931 IEA
    positive regulation of B cell differentiation GO:0045579 IEA
    prostate gland epithelium morphogenesis GO:0060740 IEA
    taurine metabolic process GO:0019530 ISS
    regulation of epithelial cell differentiation GO:0030856 IEA
    T cell differentiation in thymus GO:0033077 IEA
    cellular response to granulocyte macrophage colony-stimulating factor stimulus GO:0097011 IEA
    positive regulation of multicellular organism growth GO:0040018 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IEA
    JAK-STAT cascade GO:0007259 TAS
    positive regulation of gamma-delta T cell differentiation GO:0045588 IEA
    regulation of transcription from RNA polymerase II promoter GO:0006357 TAS
    transcription, DNA-templated GO:0006351 IEA
    epithelial cell differentiation involved in prostate gland development GO:0060742 IEA
    positive regulation of mast cell proliferation GO:0070668 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified STAT5A in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for STAT5A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PTPN11 5781
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
2 ESR2  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
3 NMI  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
4 MAPK1 5594
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
5 CENPJ 55835
Invivo Homo sapiens
Two-hybrid Homo sapiens
6 IL2RG  
Affinity Capture-MS Homo sapiens
7 PPIB 5479
Affinity Capture-MS Homo sapiens
8 PRTFDC1 56952
Two-hybrid Homo sapiens
9 NR3C1 2908
Affinity Capture-MS Homo sapiens
10 ERBB4 2066
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
11 EGFR 1956
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
12 NCOR2  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
13 CD247 919
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
14 ESR1  
Reconstituted Complex Homo sapiens
15 JAK3 3718
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
16 BRCA2 675
Affinity Capture-Western Homo sapiens
17 JAK1 3716
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
18 CRKL 1399
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
19 IL2RB  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
20 SRC 6714
Affinity Capture-MS Homo sapiens
21 SYK 6850
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
22 MAGEA12  
Two-hybrid Homo sapiens
23 BMX  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
24 JAK2 3717
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
25 BRCA1 672
Affinity Capture-Western Homo sapiens
26 TSLP  
Invivo Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
27 EPOR  
Invivo Homo sapiens
Invitro Homo sapiens
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