Gene description for STAT5A
Gene name signal transducer and activator of transcription 5A
Gene symbol STAT5A
Other names/aliases MGF
STAT5
Species Homo sapiens
 Database cross references - STAT5A
ExoCarta ExoCarta_6776
Vesiclepedia VP_6776
Entrez Gene 6776
HGNC 11366
MIM 601511
UniProt P42229  
 STAT5A identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for STAT5A
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA-binding transcription factor activity GO:0003700 IDA
    protein binding GO:0005515 IPI
    DNA-binding transcription factor binding GO:0140297 IPI
Biological Process
    positive regulation of endothelial cell proliferation GO:0001938 IMP
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    defense response GO:0006952 IBA
    cell surface receptor signaling pathway via JAK-STAT GO:0007259 IBA
    cell surface receptor signaling pathway via JAK-STAT GO:0007259 NAS
    lactation GO:0007595 IEA
    cytokine-mediated signaling pathway GO:0019221 IBA
    taurine metabolic process GO:0019530 ISS
    eosinophil differentiation GO:0030222 IDA
    interleukin-15-mediated signaling pathway GO:0035723 IDA
    interleukin-4-mediated signaling pathway GO:0035771 IDA
    reelin-mediated signaling pathway GO:0038026 IDA
    interleukin-5-mediated signaling pathway GO:0038043 IDA
    interleukin-2-mediated signaling pathway GO:0038110 IDA
    interleukin-7-mediated signaling pathway GO:0038111 IDA
    interleukin-9-mediated signaling pathway GO:0038113 IDA
    interleukin-3-mediated signaling pathway GO:0038156 IDA
    thrombopoietin-mediated signaling pathway GO:0038163 IDA
    regulation of multicellular organism growth GO:0040014 ISS
    regulation of cell population proliferation GO:0042127 IBA
    response to peptide hormone GO:0043434 IBA
    positive regulation of blood vessel endothelial cell migration GO:0043536 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 NAS
    growth hormone receptor signaling pathway via JAK-STAT GO:0060397 IBA
    negative regulation of T-helper 17 cell lineage commitment GO:2000329 IDA
Subcellular Localization
    chromatin GO:0000785 ISA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IC
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    RNA polymerase II transcription regulator complex GO:0090575 IBA
    RNA polymerase II transcription regulator complex GO:0090575 NAS
 Experiment description of studies that identified STAT5A in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for STAT5A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 SHC1 6464
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
3 STAT5B 6777
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 STAT5A 6776
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
5 WASH2P  
Two-hybrid Homo sapiens
6 CRK 1398
Co-localization Homo sapiens
7 BRCA1 672
Affinity Capture-Western Homo sapiens
8 ADD3 120
Affinity Capture-MS Homo sapiens
9 PGR  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
10 PTPN1 5770
Protein-peptide Homo sapiens
11 CD247 919
Affinity Capture-Western Homo sapiens
12 NFKB1 4790
Affinity Capture-Western Homo sapiens
13 CTLA4  
Co-localization Homo sapiens
14 TCF12  
Two-hybrid Homo sapiens
15 HELZ 9931
Two-hybrid Homo sapiens
16 FBXW7  
Two-hybrid Homo sapiens
17 LEF1  
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
18 PDK2 5164
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 ERMP1 79956
Affinity Capture-MS Homo sapiens
20 AQP11  
Two-hybrid Homo sapiens
21 CSNK2B 1460
Two-hybrid Homo sapiens
22 NCOA1  
Affinity Capture-Western Homo sapiens
23 RBMX 27316
Two-hybrid Homo sapiens
24 MAPK1 5594
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
25 FARS2 10667
Affinity Capture-MS Homo sapiens
26 POM121C  
Two-hybrid Homo sapiens
27 PDGFRB 5159
Co-localization Homo sapiens
28 SOCS7  
Affinity Capture-Western Homo sapiens
29 GDF15 9518
Affinity Capture-MS Homo sapiens
30 BRCA2 675
Affinity Capture-Western Homo sapiens
31 SMARCA4 6597
Affinity Capture-Western Homo sapiens
32 DDX20 11218
Affinity Capture-MS Homo sapiens
33 CARM1 10498
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
34 CRKL 1399
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
35 CBX5 23468
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
36 PDHX 8050
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 KRT87P  
Affinity Capture-MS Homo sapiens
38 RNF13 11342
Affinity Capture-MS Homo sapiens
39 NOL4  
Affinity Capture-MS Homo sapiens
40 EPOR  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
PCA Homo sapiens
Reconstituted Complex Homo sapiens
41 FLOT2 2319
Affinity Capture-MS Homo sapiens
42 ICAM5 7087
Affinity Capture-MS Homo sapiens
43 NLK  
Co-localization Homo sapiens
44 TXNDC11 51061
Two-hybrid Homo sapiens
45 MRPS6  
Two-hybrid Homo sapiens
46 PTPN11 5781
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
47 NARF 26502
Co-localization Homo sapiens
48 ILKAP 80895
Affinity Capture-MS Homo sapiens
49 EPAS1  
Two-hybrid Homo sapiens
50 PPARG 5468
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
51 UGT2B4  
Two-hybrid Homo sapiens
52 ERBB4 2066
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
53 EGFR 1956
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
PCA Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Biochemical Activity Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
54 FN1 2335
Two-hybrid Homo sapiens
55 SF3A1 10291
Affinity Capture-MS Homo sapiens
56 FGFR2 2263
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
57 R3HDM2  
Affinity Capture-MS Homo sapiens
58 STAT1 6772
Co-localization Homo sapiens
59 JAK1 3716
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
60 GNB2 2783
Two-hybrid Homo sapiens
61 RUNX2  
Affinity Capture-Western Homo sapiens
62 TLR2 7097
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 IL2RB  
Reconstituted Complex Homo sapiens
64 ANGPTL6  
Affinity Capture-MS Homo sapiens
65 CYP2C9  
Two-hybrid Homo sapiens
66 MAGEA12  
Two-hybrid Homo sapiens
67 DLAT 1737
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 MYB  
Affinity Capture-Western Homo sapiens
69 FLOT1 10211
Affinity Capture-MS Homo sapiens
70 BTK 695
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
71 SPICE1  
Affinity Capture-MS Homo sapiens
72 GLUD1 2746
Two-hybrid Homo sapiens
73 ESR2  
Reconstituted Complex Homo sapiens
74 PDK3 5165
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 NMI  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
76 ZNF596  
Two-hybrid Homo sapiens
77 PDHA1 5160
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 PRTFDC1 56952
Two-hybrid Homo sapiens
79 SQSTM1 8878
Affinity Capture-Western Homo sapiens
80 INSR 3643
Affinity Capture-Western Homo sapiens
81 PDHB 5162
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 HDAC1 3065
Affinity Capture-Western Homo sapiens
83 KLHL7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 ESR1  
Reconstituted Complex Homo sapiens
85 JAK3 3718
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
86 GHR  
Co-localization Homo sapiens
87 JAK2 3717
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
88 PRMT1 3276
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
89 EP300 2033
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which STAT5A is involved
PathwayEvidenceSource
Cytokine Signaling in Immune system TAS Reactome
Cytokine Signaling in Immune system IEA Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
Downstream signal transduction TAS Reactome
Erythropoietin activates STAT5 IEA Reactome
FGFR1 mutant receptor activation TAS Reactome
FLT3 Signaling IEA Reactome
FLT3 signaling in disease TAS Reactome
Growth hormone receptor signaling IEA Reactome
Growth hormone receptor signaling TAS Reactome
Immune System TAS Reactome
Immune System IEA Reactome
Inactivation of CSF3 (G-CSF) signaling IEA Reactome
Interleukin-15 signaling TAS Reactome
Interleukin-2 family signaling TAS Reactome
Interleukin-2 family signaling IEA Reactome
Interleukin-2 signaling TAS Reactome
Interleukin-2 signaling IEA Reactome
Interleukin-20 family signaling TAS Reactome
Interleukin-21 signaling TAS Reactome
Interleukin-3, Interleukin-5 and GM-CSF signaling TAS Reactome
Interleukin-3, Interleukin-5 and GM-CSF signaling IEA Reactome
Interleukin-7 signaling TAS Reactome
Interleukin-7 signaling IEA Reactome
Interleukin-9 signaling TAS Reactome
Nuclear events stimulated by ALK signaling in cancer TAS Reactome
Nuclear signaling by ERBB4 TAS Reactome
Prolactin receptor signaling IEA Reactome
Signal Transduction TAS Reactome
Signal Transduction IEA Reactome
Signaling by ALK fusions and activated point mutants TAS Reactome
Signaling by ALK in cancer TAS Reactome
Signaling by CSF3 (G-CSF) IEA Reactome
Signaling by cytosolic FGFR1 fusion mutants TAS Reactome
Signaling by ERBB4 TAS Reactome
Signaling by Erythropoietin IEA Reactome
Signaling by FGFR in disease TAS Reactome
Signaling by FGFR1 in disease TAS Reactome
Signaling by FLT3 fusion proteins TAS Reactome
Signaling by FLT3 ITD and TKD mutants TAS Reactome
Signaling by Interleukins TAS Reactome
Signaling by Interleukins IEA Reactome
Signaling by KIT in disease TAS Reactome
Signaling by Leptin IEA Reactome
Signaling by PDGF TAS Reactome
Signaling by phosphorylated juxtamembrane, extracellular and kinase domain KIT mutants TAS Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by SCF-KIT TAS Reactome
STAT5 Activation IEA Reactome
STAT5 activation downstream of FLT3 ITD mutants TAS Reactome





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