Gene description for ADD3
Gene name adducin 3 (gamma)
Gene symbol ADD3
Other names/aliases ADDL
Species Homo sapiens
 Database cross references - ADD3
ExoCarta ExoCarta_120
Vesiclepedia VP_120
Entrez Gene 120
HGNC 245
MIM 601568
UniProt Q9UEY8  
 ADD3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for ADD3
Molecular Function
    protein kinase C binding GO:0005080 IEA
    structural constituent of cytoskeleton GO:0005200 IEA
    calmodulin binding GO:0005516 IEA
    actin filament binding GO:0051015 IBA
Biological Process
    cytoskeleton organization GO:0007010 IEA
    response to xenobiotic stimulus GO:0009410 IEA
    positive regulation of vasoconstriction GO:0045907 IEA
    barbed-end actin filament capping GO:0051016 IBA
    positive regulation of cytoskeleton organization GO:0051495 IEA
Subcellular Localization
    condensed nuclear chromosome GO:0000794 IEA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IBA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    brush border GO:0005903 IEA
    cell-cell junction GO:0005911 IEA
    cell cortex GO:0005938 IEA
    postsynaptic density GO:0014069 IBA
    membrane GO:0016020 TAS
 Experiment description of studies that identified ADD3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ADD3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GNL2 29889
Affinity Capture-MS Homo sapiens
2 STX7 8417
Proximity Label-MS Homo sapiens
3 ATXN1 6310
Two-hybrid Homo sapiens
4 MCAM 4162
Proximity Label-MS Homo sapiens
5 GJD3  
Proximity Label-MS Homo sapiens
6 CYP1A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 RAB35 11021
Proximity Label-MS Homo sapiens
8 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
9 Tmod3 50875
Affinity Capture-MS Mus musculus
10 KLF15  
Affinity Capture-MS Homo sapiens
11 EBAG9 9166
Proximity Label-MS Homo sapiens
12 LAMP2 3920
Proximity Label-MS Homo sapiens
13 Myh9 17886
Affinity Capture-MS Mus musculus
14 Lima1  
Affinity Capture-MS Mus musculus
15 TP53 7157
Affinity Capture-MS Homo sapiens
16 MARCKS 4082
Proximity Label-MS Homo sapiens
17 LAMP3  
Proximity Label-MS Homo sapiens
18 XPO1 7514
Affinity Capture-MS Homo sapiens
19 STAT5A 6776
Affinity Capture-MS Homo sapiens
20 GJA1 2697
Proximity Label-MS Homo sapiens
21 ACTG1 71
Affinity Capture-MS Homo sapiens
22 DYNC1I2 1781
Co-fractionation Homo sapiens
23 RAB9A 9367
Proximity Label-MS Homo sapiens
24 EPB41L4A 64097
Proximity Label-MS Homo sapiens
25 LAMP1 3916
Proximity Label-MS Homo sapiens
26 Calml3  
Affinity Capture-MS Mus musculus
27 ACTC1 70
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
28 MEF2BNB  
Affinity Capture-MS Homo sapiens
29 CUL3 8452
Affinity Capture-MS Homo sapiens
30 CAPZA2 830
Affinity Capture-MS Homo sapiens
31 G3BP1 10146
Co-fractionation Homo sapiens
32 HSPB1 3315
Two-hybrid Homo sapiens
33 MYO19  
Affinity Capture-MS Homo sapiens
34 ARF6 382
Proximity Label-MS Homo sapiens
35 FEM1A  
Affinity Capture-MS Homo sapiens
36 VWA5A 4013
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 CXADR 1525
Proximity Label-MS Homo sapiens
38 GBP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 DDX58 23586
Affinity Capture-RNA Homo sapiens
40 OCLN 100506658
Proximity Label-MS Homo sapiens
41 SLC25A41  
Affinity Capture-MS Homo sapiens
42 LMAN1 3998
Proximity Label-MS Homo sapiens
43 LYN 4067
Proximity Label-MS Homo sapiens
44 MAPRE1 22919
Affinity Capture-MS Homo sapiens
45 DNAJC5 80331
Proximity Label-MS Homo sapiens
46 CRISP3  
Affinity Capture-MS Homo sapiens
47 ELL3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 LAMTOR1 55004
Proximity Label-MS Homo sapiens
49 UBE2I 7329
Biochemical Activity Homo sapiens
50 ADD2 119
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 SYNPO 11346
Affinity Capture-MS Homo sapiens
52 CDH1 999
Proximity Label-MS Homo sapiens
53 RPA3 6119
Proximity Label-MS Homo sapiens
54 DIRAS3  
Proximity Label-MS Homo sapiens
55 DNAJC5B  
Proximity Label-MS Homo sapiens
56 HDAC11  
Affinity Capture-MS Homo sapiens
57 ANKRD29  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 RAB11A 8766
Proximity Label-MS Homo sapiens
59 RNF111 54778
Affinity Capture-MS Homo sapiens
60 DTX2 113878
Proximity Label-MS Homo sapiens
61 GPSM3  
Affinity Capture-MS Homo sapiens
62 RAB2A 5862
Proximity Label-MS Homo sapiens
63 EPHA2 1969
Proximity Label-MS Homo sapiens
64 MLPH  
Affinity Capture-MS Homo sapiens
65 OPRM1 4988
Two-hybrid Homo sapiens
66 ACTB 60
Proximity Label-MS Homo sapiens
67 CAV1 857
Proximity Label-MS Homo sapiens
68 RNF2  
Affinity Capture-MS Homo sapiens
69 RHOB 388
Proximity Label-MS Homo sapiens
70 PARK2  
Affinity Capture-MS Homo sapiens
71 ADD1 118
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 FAM78A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 C11orf52 91894
Proximity Label-MS Homo sapiens
74 RAB5C 5878
Proximity Label-MS Homo sapiens
75 NEUROG3  
Affinity Capture-MS Homo sapiens
76 AGTR1  
Two-hybrid Homo sapiens
77 KRAS 3845
Proximity Label-MS Homo sapiens
78 GIP  
Affinity Capture-MS Homo sapiens
79 FLOT1 10211
Proximity Label-MS Homo sapiens
80 STX6 10228
Proximity Label-MS Homo sapiens
81 TGM7  
Affinity Capture-MS Homo sapiens
82 ASAP2 8853
Co-fractionation Homo sapiens
View the network image/svg+xml



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