Gene description for Myh9
Gene name myosin, heavy polypeptide 9, non-muscle
Gene symbol Myh9
Other names/aliases Fltn
Myhn-1
Myhn1
NMHCIIA
NMMHC-A
NMMHC-IIA
TU72.6
Species Mus musculus
 Database cross references - Myh9
ExoCarta ExoCarta_17886
Vesiclepedia VP_17886
Entrez Gene 17886
UniProt Q8VDD5  
 Myh9 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Microglia 16081791    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Myh9
Molecular Function
    calmodulin binding GO:0005516 IEA
    ADP binding GO:0043531 ISO
    actin filament binding GO:0051015 ISO
    protein homodimerization activity GO:0042803 ISO
    protein domain specific binding GO:0019904 ISO
    poly(A) RNA binding GO:0044822 ISO
    motor activity GO:0003774 IEA
    actin-dependent ATPase activity GO:0030898 ISO
    microfilament motor activity GO:0000146 ISO
    protein binding GO:0005515 IPI
    actin binding GO:0003779 ISO
    nucleotide binding GO:0000166 IEA
    protein anchor GO:0043495 ISO
    ATPase activity GO:0016887 ISO
    ATP binding GO:0005524 ISO
Biological Process
    movement of cell or subcellular component GO:0006928 IMP
    actin cytoskeleton reorganization GO:0031532 ISO
    phagocytosis, engulfment GO:0006911 IMP
    establishment of T cell polarity GO:0001768 IMP
    cell morphogenesis involved in differentiation GO:0000904 IMP
    platelet aggregation GO:0070527 ISO
    myoblast fusion GO:0007520 IMP
    cytokinesis GO:0000910 ISO
    single organismal cell-cell adhesion GO:0016337 IMP
    regulation of cell shape GO:0008360 ISO
    meiotic spindle organization GO:0000212 IDA
    uropod organization GO:0032796 IMP
    protein transport GO:0015031 ISO
    actin filament capping GO:0051693 IDA
    establishment of meiotic spindle localization GO:0051295 IDA
    platelet formation GO:0030220 ISO
    in utero embryonic development GO:0001701 IMP
    actin filament-based movement GO:0030048 ISO
    blood vessel endothelial cell migration GO:0043534 ISO
    negative regulation of actin filament severing GO:1903919 ISO
    membrane protein ectodomain proteolysis GO:0006509 ISO
    positive regulation of protein processing in phagocytic vesicle GO:1903923 IMP
    cell adhesion GO:0007155 IMP
    angiogenesis GO:0001525 ISO
    actomyosin structure organization GO:0031032 ISO
    actin filament polymerization GO:0030041 IDA
Subcellular Localization
    integrin complex GO:0008305 ISO
    neuromuscular junction GO:0031594 IDA
    actin cytoskeleton GO:0015629 ISO
    nucleus GO:0005634 ISO
    actomyosin contractile ring GO:0005826 ISO
    focal adhesion GO:0005925 IDA
    plasma membrane GO:0005886 ISO
    cytoskeleton GO:0005856 IEA
    cell cortex GO:0005938 IDA
    extracellular exosome GO:0070062 ISO
    COP9 signalosome GO:0008180 ISO
    myosin II filament GO:0097513 ISO
    cell-cell adherens junction GO:0005913 IDA
    immunological synapse GO:0001772 ISO
    spindle GO:0005819 IDA
    brush border GO:0005903 IDA
    myosin complex GO:0016459 IDA
    cytosol GO:0005829 ISO
    ruffle GO:0001726 ISO
    cytoplasm GO:0005737 ISO
    uropod GO:0001931 ISO
    actomyosin GO:0042641 ISO
    cell leading edge GO:0031252 ISO
    myosin II complex GO:0016460 ISO
    stress fiber GO:0001725 ISO
    membrane GO:0016020 ISO
    protein complex GO:0043234 ISO
    cortical cytoskeleton GO:0030863 IDA
    cleavage furrow GO:0032154 ISO
 Experiment description of studies that identified Myh9 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD81|FLOT1
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL.
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 214
MISEV standards
✔
EM
EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 215
MISEV standards
✔
EM
EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 216
MISEV standards
✔
EM
EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 14
MISEV standards
✔
EM
EV Biophysical techniques
✔
RAB7|RAB11|CD9|CD63|LAMP1|LAMP2
EV Enriched markers
✔
DNM
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
7
Experiment ID 188
MISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|UCHL1|HSP90
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 908
MISEV standards
✔
EM
EV Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
✔
HSPA5
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Myh9
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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