Gene description for SIPA1
Gene name signal-induced proliferation-associated 1
Gene symbol SIPA1
Other names/aliases SPA1
Species Homo sapiens
 Database cross references - SIPA1
ExoCarta ExoCarta_6494
Vesiclepedia VP_6494
Entrez Gene 6494
HGNC 10885
MIM 602180
UniProt Q96FS4  
 SIPA1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for SIPA1
Molecular Function
    GTPase activator activity GO:0005096 EXP
    GTPase activator activity GO:0005096 IBA
    GTPase activator activity GO:0005096 NAS
    protein binding GO:0005515 IPI
Biological Process
    adaptive immune response GO:0002250 TAS
    cytoskeleton organization GO:0007010 NAS
    negative regulation of cell adhesion GO:0007162 NAS
    signal transduction GO:0007165 TAS
    negative regulation of cell growth GO:0030308 NAS
    intracellular signal transduction GO:0035556 NAS
    cellular response to water deprivation GO:0042631 IEA
    negative regulation of cell cycle GO:0045786 NAS
    regulation of small GTPase mediated signal transduction GO:0051056 IEA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    protein-containing complex GO:0032991 IEA
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified SIPA1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SIPA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
2 ATG16L1 55054
Affinity Capture-MS Homo sapiens
3 ANLN 54443
Affinity Capture-MS Homo sapiens
4 TRIM21 6737
Affinity Capture-MS Homo sapiens
5 MYO1C 4641
Affinity Capture-MS Homo sapiens
6 DVL1 1855
Two-hybrid Homo sapiens
7 FMNL1 752
Affinity Capture-MS Homo sapiens
8 PPP1CB 5500
Affinity Capture-MS Homo sapiens
9 Anapc13  
Affinity Capture-MS Mus musculus
10 BKRF1  
Affinity Capture-MS
11 Myh9 17886
Affinity Capture-MS Mus musculus
12 KIF20A 10112
Affinity Capture-MS Homo sapiens
13 YWHAB 7529
Affinity Capture-MS Homo sapiens
14 CHMP4C 92421
Affinity Capture-MS Homo sapiens
15 Calml3  
Affinity Capture-MS Mus musculus
16 DBN1 1627
Affinity Capture-MS Homo sapiens
17 SIPA1L3  
Affinity Capture-MS Homo sapiens
18 LIMA1 51474
Affinity Capture-MS Homo sapiens
19 CAPZA2 830
Affinity Capture-MS Homo sapiens
20 MYO19  
Affinity Capture-MS Homo sapiens
21 CDKN1A  
Two-hybrid Homo sapiens
22 Actb 11461
Affinity Capture-MS Mus musculus
23 TAF1  
Affinity Capture-MS Homo sapiens
24 DDX58 23586
Affinity Capture-RNA Homo sapiens
25 FN1 2335
Affinity Capture-MS Homo sapiens
26 LZTS2 84445
Affinity Capture-MS Homo sapiens
27 YWHAZ 7534
Affinity Capture-MS Homo sapiens
28 MYH9 4627
Affinity Capture-MS Homo sapiens
29 SIPA1L2  
Affinity Capture-MS Homo sapiens
30 YWHAQ 10971
Affinity Capture-MS Homo sapiens
31 IQGAP1 8826
Affinity Capture-MS Homo sapiens
32 YWHAG 7532
Affinity Capture-MS Homo sapiens
33 BRD4 23476
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which SIPA1 is involved
PathwayEvidenceSource
Adaptive Immune System TAS Reactome
Immune System TAS Reactome
Rap1 signalling TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here