Gene description for FMNL1
Gene name formin-like 1
Gene symbol FMNL1
Other names/aliases C17orf1
C17orf1B
FHOD4
FMNL
KW-13
Species Homo sapiens
 Database cross references - FMNL1
ExoCarta ExoCarta_752
Vesiclepedia VP_752
Entrez Gene 752
HGNC 1212
MIM 604656
UniProt O95466  
 FMNL1 identified in sEVs derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 35918900    
Breast cancer cells 35918900    
Hepatocellular carcinoma cells 26054723    
Thymus 23844026    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for FMNL1
Molecular Function
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 ISS
    GTPase activating protein binding GO:0032794 IDA
    actin filament binding GO:0051015 IBA
    actin filament binding GO:0051015 ISS
Biological Process
    regulation of cell shape GO:0008360 IBA
    regulation of cell shape GO:0008360 IMP
    cell migration GO:0016477 IBA
    cortical actin cytoskeleton organization GO:0030866 IBA
    cortical actin cytoskeleton organization GO:0030866 IMP
    actin filament severing GO:0051014 ISS
Subcellular Localization
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISS
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 ISS
    cell cortex GO:0005938 IEA
    membrane GO:0016020 HDA
    bleb GO:0032059 IEA
    phagocytic vesicle GO:0045335 ISS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified FMNL1 in sEVs
1
Experiment ID 79
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 1334
MISEV standards
Biophysical techniques
✔
CD9
Enriched markers
✔
ACTB
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35918900    
Organism Homo sapiens
Experiment description Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL)
Authors "Bauzá-Martinez J, Armony G, Pronker MF, Wu W. "
Journal name J Extracell Vesicles
Publication year 2022
Sample Breast cancer cells
Sample name LM2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectometry
8
Experiment ID 1336
MISEV standards
Biophysical techniques
✔
CD9
Enriched markers
✔
ACTB
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35918900    
Organism Homo sapiens
Experiment description Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL)
Authors "Bauzá-Martinez J, Armony G, Pronker MF, Wu W. "
Journal name J Extracell Vesicles
Publication year 2022
Sample Breast cancer cells
Sample name LM2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectometry
9
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
10
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for FMNL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RPL11 6135
Affinity Capture-MS Homo sapiens
2 ATG16L1 55054
Affinity Capture-MS Homo sapiens
3 RALY 22913
Affinity Capture-MS Homo sapiens
4 ARHGAP17 55114
Affinity Capture-MS Homo sapiens
5 FLI1  
Affinity Capture-MS Homo sapiens
6 PFN2 5217
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
7 LSP1 4046
Affinity Capture-MS Homo sapiens
8 Mcm3 17215
Affinity Capture-MS Mus musculus
9 OTUD4  
Affinity Capture-MS Homo sapiens
10 CWF19L1 55280
Affinity Capture-MS Homo sapiens
11 FMNL1 752
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 TOMM40 10452
Affinity Capture-MS Homo sapiens
13 Tubg1 103733
Affinity Capture-MS Mus musculus
14 PARP1 142
Affinity Capture-MS Homo sapiens
15 ABCB8 11194
Affinity Capture-MS Homo sapiens
16 TMPO 7112
Affinity Capture-MS Homo sapiens
17 PLEC 5339
Affinity Capture-MS Homo sapiens
18 NUFIP2 57532
Affinity Capture-MS Homo sapiens
19 SIPA1 6494
Affinity Capture-MS Homo sapiens
20 KCTD10 83892
Affinity Capture-MS Homo sapiens
21 TCERG1 10915
Reconstituted Complex Homo sapiens
22 PFN1 5216
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
23 TUFM 7284
Affinity Capture-MS Homo sapiens
24 LMO7 4008
Affinity Capture-MS Homo sapiens
25 AHNAK 79026
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
26 CAP1 10487
Affinity Capture-MS Homo sapiens
27 ABCF1 23
Affinity Capture-MS Homo sapiens
28 MTCH2 23788
Affinity Capture-MS Homo sapiens
29 LIMA1 51474
Affinity Capture-MS Homo sapiens
30 MS4A1 931
Affinity Capture-MS Homo sapiens
31 STRN3 29966
Proximity Label-MS Homo sapiens
32 THOC5 8563
Affinity Capture-MS Homo sapiens
33 PRPF40A 55660
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
34 CHCHD3 54927
Affinity Capture-MS Homo sapiens
35 HSP90B1 7184
Affinity Capture-MS Homo sapiens
36 POLR3E 55718
Affinity Capture-MS Homo sapiens
37 CORO1A 11151
Affinity Capture-MS Homo sapiens
38 TRIM54  
Two-hybrid Homo sapiens
39 Cdc20  
Affinity Capture-MS Mus musculus
40 ATAD3A 55210
Affinity Capture-MS Homo sapiens
41 STOM 2040
Affinity Capture-MS Homo sapiens
42 COPB1 1315
Affinity Capture-MS Homo sapiens
43 PPP1R10  
Affinity Capture-MS Homo sapiens
44 PHB2 11331
Affinity Capture-MS Homo sapiens
45 HSPA1B 3304
Affinity Capture-MS Homo sapiens
46 PPP1R12A 4659
Affinity Capture-MS Homo sapiens
47 ACTR5 79913
Affinity Capture-MS Homo sapiens
48 Otud5  
Affinity Capture-MS Mus musculus
49 ZNF787  
Affinity Capture-MS Homo sapiens
50 FLNB 2317
Affinity Capture-MS Homo sapiens
51 ARHGAP4 393
Affinity Capture-MS Homo sapiens
52 SSRP1 6749
Affinity Capture-MS Homo sapiens
53 ACTN4 81
Affinity Capture-MS Homo sapiens
54 HSPA5 3309
Affinity Capture-MS Homo sapiens
55 GEMIN5 25929
Affinity Capture-MS Homo sapiens
56 XRCC6 2547
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
57 FLII 2314
Affinity Capture-MS Homo sapiens
58 TIA1 7072
Affinity Capture-MS Homo sapiens
59 CD44 960
Affinity Capture-MS Homo sapiens
60 Tnks2  
Affinity Capture-MS Mus musculus
61 NPM1 4869
Affinity Capture-MS Homo sapiens
62 RAC1 5879
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
63 Prkaa1  
Affinity Capture-MS Mus musculus
64 GAS7  
Reconstituted Complex Homo sapiens
65 HMG20A  
Two-hybrid Homo sapiens
66 CORO1C 23603
Affinity Capture-MS Homo sapiens
67 SUPT16H 11198
Affinity Capture-MS Homo sapiens
68 APP 351
Reconstituted Complex Homo sapiens
69 ATP5B 506
Affinity Capture-MS Homo sapiens
70 XRCC5 7520
Affinity Capture-MS Homo sapiens
71 TAP2 6891
Affinity Capture-MS Homo sapiens
72 SIPA1L3  
Affinity Capture-MS Homo sapiens
73 VDAC1 7416
Affinity Capture-MS Homo sapiens
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