Gene description for ARHGAP17
Gene name Rho GTPase activating protein 17
Gene symbol ARHGAP17
Other names/aliases MST066
MST110
MSTP038
MSTP066
MSTP110
NADRIN
PP367
PP4534
RICH-1
RICH1
WBP15
Species Homo sapiens
 Database cross references - ARHGAP17
ExoCarta ExoCarta_55114
Vesiclepedia VP_55114
Entrez Gene 55114
HGNC 18239
MIM 608293
UniProt Q68EM7  
 ARHGAP17 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for ARHGAP17
Molecular Function
    GTPase activator activity GO:0005096 IBA
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 IEA
Biological Process
    actin filament organization GO:0007015 IEA
    signal transduction GO:0007165 IEA
    calcium-ion regulated exocytosis GO:0017156 IEA
    regulation of actin cytoskeleton organization GO:0032956 IBA
    regulation of Rac protein signal transduction GO:0035020 IBA
    negative regulation of small GTPase mediated signal transduction GO:0051058 IBA
Subcellular Localization
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    bicellular tight junction GO:0005923 IEA
 Experiment description of studies that identified ARHGAP17 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
10
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ARHGAP17
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PARD3 56288
Affinity Capture-Western Homo sapiens
2 DDX6 1656
Co-fractionation Homo sapiens
3 WDR49 151790
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 GLS 2744
Co-fractionation Homo sapiens
5 ARRB2 409
Affinity Capture-MS Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 FMNL1 752
Affinity Capture-MS Homo sapiens
8 ALDOC 230
Co-fractionation Homo sapiens
9 COMMD4 54939
Co-fractionation Homo sapiens
10 PACSIN1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
11 LAMTOR1 55004
Proximity Label-MS Homo sapiens
12 INADL 10207
Affinity Capture-MS Homo sapiens
13 DLG3 1741
Affinity Capture-MS Homo sapiens
14 EIF3K 27335
Affinity Capture-MS Homo sapiens
15 BAG2 9532
Affinity Capture-MS Homo sapiens
16 PFN1 5216
Proximity Label-MS Homo sapiens
17 STXBP1 6812
Co-fractionation Homo sapiens
18 CRK 1398
Reconstituted Complex Homo sapiens
19 PRKCI 5584
Affinity Capture-Western Homo sapiens
20 CTTN 2017
Reconstituted Complex Homo sapiens
21 S100A2 6273
Affinity Capture-MS Homo sapiens
22 CHMP7 91782
Co-fractionation Homo sapiens
23 LCK 3932
Reconstituted Complex Homo sapiens
24 COMMD2 51122
Co-fractionation Homo sapiens
25 SLC9A3R2 9351
Affinity Capture-MS Homo sapiens
26 GALNT16 57452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 OXSR1 9943
Co-fractionation Homo sapiens
28 CAPZA1 829
Affinity Capture-MS Homo sapiens
29 TRIM68 55128
Affinity Capture-MS Homo sapiens
30 EIF4EBP2 1979
Co-fractionation Homo sapiens
31 DNMBP 23268
Two-hybrid Homo sapiens
32 PIK3R1 5295
Reconstituted Complex Homo sapiens
33 MAGEA9  
Affinity Capture-MS Homo sapiens
34 NLRP3  
Co-fractionation Homo sapiens
35 APP 351
Reconstituted Complex Homo sapiens
36 SH3BP1 23616
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 MAPRE1 22919
Proximity Label-MS Homo sapiens
38 NXF1 10482
Affinity Capture-RNA Homo sapiens
39 CDC42 998
Biochemical Activity Homo sapiens
40 SH3GLB1 51100
Reconstituted Complex Homo sapiens
41 CAPZB 832
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
42 ABL1 25
Reconstituted Complex Homo sapiens
43 NIPSNAP1 8508
Affinity Capture-MS Homo sapiens
44 ARHGAP44  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 NRAS 4893
Co-fractionation Homo sapiens
46 KLC1 3831
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 VCL 7414
Affinity Capture-MS Homo sapiens
48 RPA3 6119
Proximity Label-MS Homo sapiens
49 SEC31A 22872
Co-fractionation Homo sapiens
50 VCP 7415
Affinity Capture-MS Homo sapiens
51 NTRK1 4914
Affinity Capture-MS Homo sapiens
52 PAPSS1 9061
Co-fractionation Homo sapiens
53 MPP5 64398
Affinity Capture-MS Homo sapiens
54 ACTR3 10096
Co-fractionation Homo sapiens
55 SLC9A3R1 9368
Affinity Capture-MS Homo sapiens
56 Trp53bp1  
Affinity Capture-MS Mus musculus
57 ARPC2 10109
Co-fractionation Homo sapiens
58 TRIP10 9322
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
59 OPA1 4976
Co-fractionation Homo sapiens
60 AMOT 154796
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
61 ACTB 60
Proximity Label-MS Homo sapiens
62 SRC 6714
Reconstituted Complex Homo sapiens
63 FNBP1 23048
Reconstituted Complex Homo sapiens
64 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 BTK 695
Reconstituted Complex Homo sapiens
66 RAC1 5879
Biochemical Activity Homo sapiens
67 PDZK1 5174
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 WARS 7453
Co-fractionation Homo sapiens
69 PARD6A  
Affinity Capture-Western Homo sapiens
70 CKB 1152
Affinity Capture-MS Homo sapiens
71 RHOB 388
Proximity Label-MS Homo sapiens
72 GRB2 2885
Reconstituted Complex Homo sapiens
73 COMT 1312
Co-fractionation Homo sapiens
74 CD2AP 23607
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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