Gene description for AAK1
Gene name AP2 associated kinase 1
Gene symbol AAK1
Other names/aliases -
Species Homo sapiens
 Database cross references - AAK1
ExoCarta ExoCarta_22848
Vesiclepedia VP_22848
Entrez Gene 22848
HGNC 19679
UniProt Q2M2I8  
 AAK1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for AAK1
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 ISS
    protein serine/threonine kinase activity GO:0004674 TAS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    Notch binding GO:0005112 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    AP-2 adaptor complex binding GO:0035612 IBA
    AP-2 adaptor complex binding GO:0035612 IDA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    regulation of protein localization GO:0032880 IDA
    positive regulation of Notch signaling pathway GO:0045747 IDA
    protein stabilization GO:0050821 IDA
    membrane organization GO:0061024 TAS
    presynaptic endocytosis GO:0140238 IEA
    regulation of clathrin-dependent endocytosis GO:2000369 IBA
    regulation of clathrin-dependent endocytosis GO:2000369 IDA
    regulation of clathrin-dependent endocytosis GO:2000369 IMP
Subcellular Localization
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    clathrin-coated pit GO:0005905 ISS
    clathrin-coated vesicle GO:0030136 ISS
    cell leading edge GO:0031252 ISS
    terminal bouton GO:0043195 ISS
    intracellular membrane-bounded organelle GO:0043231 IDA
    presynapse GO:0098793 IBA
 Experiment description of studies that identified AAK1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for AAK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEC24B 10427
Affinity Capture-MS Homo sapiens
2 ANLN 54443
Affinity Capture-MS Homo sapiens
3 RALBP1 10928
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 GTSE1 51512
Affinity Capture-MS Homo sapiens
5 HSPA5 3309
Affinity Capture-MS Homo sapiens
6 AP2B1 163
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 IMPDH2 3615
Proximity Label-MS Homo sapiens
8 Myh9 17886
Affinity Capture-MS Mus musculus
9 Lima1  
Affinity Capture-MS Mus musculus
10 FBXL6  
Proximity Label-MS Homo sapiens
11 PLEC 5339
Affinity Capture-MS Homo sapiens
12 AP2S1 1175
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 PRNP 5621
Affinity Capture-MS Homo sapiens
14 XPO1 7514
Affinity Capture-MS Homo sapiens
15 BAG2 9532
Affinity Capture-MS Homo sapiens
16 URGCP 55665
Affinity Capture-MS Homo sapiens
17 PFN1 5216
Proximity Label-MS Homo sapiens
18 ATP13A2  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
19 Myo1c 17913
Affinity Capture-MS Mus musculus
20 GAK 2580
Affinity Capture-MS Homo sapiens
21 NUP88 4927
Affinity Capture-MS Homo sapiens
22 WNK1 65125
Affinity Capture-MS Homo sapiens
23 PRPH 5630
Proximity Label-MS Homo sapiens
24 HOOK3 84376
Proximity Label-MS Homo sapiens
25 RBM12 10137
Affinity Capture-MS Homo sapiens
26 DVL3 1857
Affinity Capture-MS Homo sapiens
27 Actb 11461
Affinity Capture-MS Mus musculus
28 CLTB 1212
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
29 APP 351
Reconstituted Complex Homo sapiens
30 Sec24c  
Affinity Capture-MS Mus musculus
31 AP2M1 1173
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 LUZP1 7798
Affinity Capture-MS Homo sapiens
33 NECAP2 55707
Affinity Capture-MS Homo sapiens
34 UBE2D2 7322
Affinity Capture-MS Homo sapiens
35 C10orf88  
Affinity Capture-MS Homo sapiens
36 CDH1 999
Proximity Label-MS Homo sapiens
37 EYA4 2070
Affinity Capture-MS Homo sapiens
38 REPS1 85021
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 PPIP5K2 23262
Affinity Capture-MS Homo sapiens
40 NTRK1 4914
Affinity Capture-MS Homo sapiens
41 CLTA 1211
Proximity Label-MS Homo sapiens
42 AP2A1 160
Affinity Capture-MS Homo sapiens
43 DIEXF  
Affinity Capture-MS Homo sapiens
44 AP1B1 162
Affinity Capture-MS Homo sapiens
45 BICD2 23299
Proximity Label-MS Homo sapiens
46 BICD1 636
Proximity Label-MS Homo sapiens
47 RUNDC3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 CLTC 1213
Affinity Capture-MS Homo sapiens
49 CCDC88A 55704
Affinity Capture-MS Homo sapiens
50 Ppp1cb 19046
Affinity Capture-MS Mus musculus
51 RPA3 6119
Proximity Label-MS Homo sapiens
52 QSER1  
Affinity Capture-MS Homo sapiens
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