Gene description for REPS1
Gene name RALBP1 associated Eps domain containing 1
Gene symbol REPS1
Other names/aliases RALBP1
Species Homo sapiens
 Database cross references - REPS1
ExoCarta ExoCarta_85021
Vesiclepedia VP_85021
Entrez Gene 85021
HGNC 15578
MIM 614825
UniProt Q96D71  
 REPS1 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for REPS1
Molecular Function
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 IPI
Biological Process
    endocytosis GO:0006897 IBA
    endosomal transport GO:0016197 IBA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    clathrin-coated pit GO:0005905 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified REPS1 in exosomes
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for REPS1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ARHGEF7 8874
Affinity Capture-MS Homo sapiens
2 AP2S1 1175
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 RALBP1 10928
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
4 GTSE1 51512
Affinity Capture-MS Homo sapiens
5 MED19  
Affinity Capture-MS Homo sapiens
6 AP2B1 163
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 WHAMMP3  
Affinity Capture-MS Homo sapiens
8 DAB2 1601
Affinity Capture-MS Homo sapiens
9 ITSN1 6453
Affinity Capture-Western Homo sapiens
10 CLINT1 9685
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 AAK1 22848
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 MARCKS 4082
Proximity Label-MS Homo sapiens
13 LDHB 3945
Affinity Capture-MS Homo sapiens
14 RUNDC3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 ATF4  
Two-hybrid Homo sapiens
16 LSR 51599
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 PIN1 5300
Reconstituted Complex Homo sapiens
18 WWC2  
Two-hybrid Homo sapiens
19 EZR 7430
Proximity Label-MS Homo sapiens
20 ABL1 25
Affinity Capture-MS Homo sapiens
21 RNF6  
Affinity Capture-MS Homo sapiens
22 PIP 5304
Affinity Capture-MS Homo sapiens
23 LIMS1 3987
Affinity Capture-MS Homo sapiens
24 LDHA 3939
Affinity Capture-MS Homo sapiens
25 C9orf78 51759
Affinity Capture-MS Homo sapiens
26 Numb  
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
27 ARHGAP18 93663
Affinity Capture-MS Homo sapiens
28 CAPZB 832
Affinity Capture-MS Homo sapiens
29 AKT1 207
Biochemical Activity Homo sapiens
30 HIP1R 9026
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 SLC25A41  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 SBDS 51119
Affinity Capture-MS Homo sapiens
33 MAPRE1 22919
Affinity Capture-MS Homo sapiens
34 AP2M1 1173
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 AMPH 273
Affinity Capture-Western Homo sapiens
36 MIF 4282
Affinity Capture-MS Homo sapiens
37 C10orf88  
Affinity Capture-MS Homo sapiens
38 REPS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 ITSN2 50618
Two-hybrid Homo sapiens
40 ARHGAP26 23092
Affinity Capture-MS Homo sapiens
41 RNPS1 10921
Affinity Capture-MS Homo sapiens
42 AP2A1 160
Affinity Capture-MS Homo sapiens
43 MYO6 4646
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 REPS1 85021
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
45 PNMA1 9240
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 C16orf72 29035
Affinity Capture-MS Homo sapiens
47 NTRK1 4914
Affinity Capture-MS Homo sapiens
48 RXRA 6256
Affinity Capture-MS Homo sapiens
49 TRIM11  
Affinity Capture-MS Homo sapiens
50 CLTA 1211
Affinity Capture-MS Homo sapiens
51 AP2A2 161
Affinity Capture-MS Homo sapiens
52 TRAPPC2L 51693
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
53 RAB11FIP2  
Affinity Capture-Western Homo sapiens
54 PNMA2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 AP1B1 162
Affinity Capture-MS Homo sapiens
56 HUWE1 10075
Affinity Capture-MS Homo sapiens
57 BMP2K 55589
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 COL8A1 1295
Affinity Capture-MS Homo sapiens
59 CCDC101  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 SGIP1  
Affinity Capture-Western Homo sapiens
61 TGIF2LY  
Affinity Capture-MS Homo sapiens
62 CLTC 1213
Affinity Capture-MS Homo sapiens
63 STAMBPL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 STAMBP 10617
Affinity Capture-MS Homo sapiens
65 ENTHD1  
Affinity Capture-MS Homo sapiens
66 MED14  
Affinity Capture-MS Homo sapiens
67 DNAJC6  
Affinity Capture-MS Homo sapiens
68 STX11 8676
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 MED9  
Affinity Capture-MS Homo sapiens
70 LRFN4  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here