Gene ontology annotations for ARHGEF7
Experiment description of studies that identified ARHGEF7 in exosomes
1
Experiment ID
489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 6
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
2
Experiment ID
490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 7
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
3
Experiment ID
491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 8
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
4
Experiment ID
492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 9
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
5
Experiment ID
363
MISEV standards
✘
Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
6
Experiment ID
364
MISEV standards
✘
Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
7
Experiment ID
365
MISEV standards
✘
Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
8
Experiment ID
488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Homo sapiens
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Mesenchymal stem cells
Sample name
UCMSC
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
9
Experiment ID
211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
IGROV1
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
10
Experiment ID
212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
OVCAR-3
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
11
Experiment ID
834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35333565
Organism
Homo sapiens
Experiment description
LAMP2A regulates the loading of proteins into exosomes
Authors
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name
Sci Adv
Publication year
2022
Sample
Retinal pigment epithelial cells
Sample name
ARPE-19
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
12
Experiment ID
835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35333565
Organism
Homo sapiens
Experiment description
LAMP2A regulates the loading of proteins into exosomes
Authors
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name
Sci Adv
Publication year
2022
Sample
Retinal pigment epithelial cells
Sample name
ARPE-19
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
Protein-protein interactions for ARHGEF7
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
YWHAH
7533
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
2
SLC9A3R2
9351
Affinity Capture-MS
Homo sapiens
3
Mbp
17196
Protein-peptide
Mus musculus
4
TPR
7175
Co-fractionation
Homo sapiens
5
FBXW7
Affinity Capture-MS
Homo sapiens
6
PRRG4
Reconstituted Complex
Homo sapiens
7
MECP2
4204
Affinity Capture-MS
Homo sapiens
8
PFDN1
5201
Affinity Capture-MS
Homo sapiens
9
SNX27
81609
Affinity Capture-Western
Homo sapiens
Affinity Capture-MS
Homo sapiens
10
TRAPPC4
51399
Affinity Capture-MS
Homo sapiens
11
CBLB
868
Co-crystal Structure
Homo sapiens
12
ZNHIT1
Affinity Capture-MS
Homo sapiens
13
TPP2
7174
Affinity Capture-MS
Homo sapiens
14
NCK1
4690
Affinity Capture-MS
Homo sapiens
15
SHANK3
85358
Affinity Capture-Western
Homo sapiens
Two-hybrid
Homo sapiens
16
TIMM13
26517
Affinity Capture-MS
Homo sapiens
17
GRB2
2885
Affinity Capture-MS
Homo sapiens
18
ICAM1
3383
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
19
KPNA1
3836
Affinity Capture-MS
Homo sapiens
20
CDC42
998
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
21
MED4
29079
Affinity Capture-MS
Homo sapiens
22
PPM1E
Reconstituted Complex
Homo sapiens
23
PAK1
5058
Affinity Capture-Western
Homo sapiens
Two-hybrid
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-Luminescence
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-Luminescence
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
24
PAK3
Two-hybrid
Homo sapiens
Affinity Capture-MS
Homo sapiens
25
KLHL1
Affinity Capture-MS
Homo sapiens
26
SHANK2
22941
Affinity Capture-Western
Homo sapiens
Two-hybrid
Homo sapiens
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
27
ZYX
7791
Affinity Capture-MS
Homo sapiens
28
GIT2
9815
Affinity Capture-MS
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
29
PAK2
5062
Reconstituted Complex
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
30
SKP1
6500
Affinity Capture-MS
Homo sapiens
31
TRIO
7204
Affinity Capture-MS
Homo sapiens
32
SHANK1
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
Two-hybrid
Homo sapiens
33
TUBB3
10381
Two-hybrid
Homo sapiens
34
TRAPPC2L
51693
Affinity Capture-MS
Homo sapiens
35
EGFR
1956
Two-hybrid
Homo sapiens
PCA
Homo sapiens
36
CALM1
801
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
37
PARK2
Affinity Capture-MS
Homo sapiens
38
C1orf106
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
39
ADAM15
8751
Reconstituted Complex
Homo sapiens
40
DDX58
23586
Affinity Capture-RNA
Homo sapiens
41
YWHAE
7531
Affinity Capture-MS
Homo sapiens
42
VHL
Two-hybrid
Homo sapiens
43
LPXN
9404
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
44
NCK2
8440
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
45
CBL
867
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
Two-hybrid
Homo sapiens
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
46
NCKIPSD
51517
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
47
PYGM
5837
Affinity Capture-MS
Homo sapiens
48
TMEM154
Affinity Capture-MS
Homo sapiens
49
C12orf49
Negative Genetic
Homo sapiens
50
CAMK4
814
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
51
TAX1BP3
30851
Affinity Capture-MS
Homo sapiens
52
MYL6B
140465
Affinity Capture-MS
Homo sapiens
53
GIT1
28964
Two-hybrid
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
54
REPS1
85021
Affinity Capture-MS
Homo sapiens
55
LRRK2
120892
Biochemical Activity
Homo sapiens
Affinity Capture-Western
Homo sapiens
Co-localization
Homo sapiens
56
ZBTB33
Affinity Capture-MS
Homo sapiens
57
ACOX3
8310
Affinity Capture-MS
Homo sapiens
58
NTRK1
4914
Affinity Capture-MS
Homo sapiens
59
EXOC1
55763
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
60
PXN
5829
Proximity Label-MS
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
61
SYNC
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
62
C20orf27
54976
Affinity Capture-MS
Homo sapiens
63
ERLIN1
10613
Affinity Capture-MS
Homo sapiens
64
Zbtb48
Affinity Capture-MS
Mus musculus
65
DNPEP
23549
Affinity Capture-MS
Homo sapiens
66
KDM2A
Co-fractionation
Homo sapiens
67
YWHAG
7532
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
68
MEOX2
Two-hybrid
Homo sapiens
69
CENPQ
Affinity Capture-MS
Homo sapiens
70
SCRIB
23513
Affinity Capture-Western
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
71
ARHGEF6
9459
Affinity Capture-Western
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
72
FBXO28
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
73
PSMB6
5694
Affinity Capture-MS
Homo sapiens
74
RAC1
5879
Affinity Capture-MS
Homo sapiens
75
HSPB1
3315
Two-hybrid
Homo sapiens
76
SNRPF
6636
Affinity Capture-MS
Homo sapiens
77
ATPIF1
93974
Affinity Capture-MS
Homo sapiens
78
RFX1
Affinity Capture-MS
Homo sapiens
79
LATS1
Affinity Capture-Western
Homo sapiens
80
ITCH
83737
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
Co-crystal Structure
Homo sapiens
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
81
SLC33A1
9197
Affinity Capture-MS
Homo sapiens
82
KLHL2
11275
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
83
POTEB3
102724631
Affinity Capture-MS
Homo sapiens
84
PPM1F
9647
Reconstituted Complex
Homo sapiens
View the network
image/svg+xml
Pathways in which ARHGEF7 is involved