Gene description for ARHGEF7
Gene name Rho guanine nucleotide exchange factor (GEF) 7
Gene symbol ARHGEF7
Other names/aliases BETA-PIX
COOL-1
COOL1
Nbla10314
P50
P50BP
P85
P85COOL1
P85SPR
PAK3
PIXB
Species Homo sapiens
 Database cross references - ARHGEF7
ExoCarta ExoCarta_8874
Entrez Gene 8874
HGNC 15607
MIM 605477
UniProt Q14155  
 ARHGEF7 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for ARHGEF7
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    Rho guanyl-nucleotide exchange factor activity GO:0005089 IEA
    protein kinase binding GO:0019901 IPI
    protein binding GO:0005515 IPI
Biological Process
    hematopoietic progenitor cell differentiation GO:0002244 IEA
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 IMP
    positive regulation of GTPase activity GO:0043547 IMP
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    positive regulation of apoptotic process GO:0043065 TAS
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    negative regulation of epidermal growth factor receptor signaling pathway GO:0042059 TAS
    apoptotic signaling pathway GO:0097190 TAS
    focal adhesion assembly GO:0048041 IDA
    signal transduction GO:0007165 TAS
    axon guidance GO:0007411 TAS
    ephrin receptor signaling pathway GO:0048013 TAS
    lamellipodium assembly GO:0030032 ISS
    positive regulation of lamellipodium morphogenesis GO:2000394 IMP
    positive regulation of fibroblast migration GO:0010763 IDA
Subcellular Localization
    neuronal cell body GO:0043025 IDA
    neuron projection GO:0043005 IDA
    focal adhesion GO:0005925 IDA
    protein complex GO:0043234 IDA
    ruffle GO:0001726 IEA
    cell cortex GO:0005938 IEA
    lamellipodium GO:0030027 ISS
    cytosol GO:0005829 TAS
 Experiment description of studies that identified ARHGEF7 in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ARHGEF7
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SHANK2 22941
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
2 CBL 867
Two-hybrid Homo sapiens
3 PAK1  
Invivo Homo sapiens
Invitro Homo sapiens
4 PAK2 5062
Invivo Homo sapiens
Invitro Homo sapiens
5 SHANK1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
6 TUBB3 10381
Two-hybrid Homo sapiens
7 GIT1 28964
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
8 SHANK3 85358
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
9 PPM1F  
Invivo Homo sapiens
10 PAK3  
Invivo Homo sapiens
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