Gene description for ARHGEF7
Gene name Rho guanine nucleotide exchange factor (GEF) 7
Gene symbol ARHGEF7
Other names/aliases BETA-PIX
COOL-1
COOL1
Nbla10314
P50
P50BP
P85
P85COOL1
P85SPR
PAK3
PIXB
Species Homo sapiens
 Database cross references - ARHGEF7
ExoCarta ExoCarta_8874
Vesiclepedia VP_8874
Entrez Gene 8874
HGNC 15607
MIM 605477
UniProt Q14155  
 ARHGEF7 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for ARHGEF7
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 EXP
    guanyl-nucleotide exchange factor activity GO:0005085 IBA
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IPI
    gamma-tubulin binding GO:0043015 IDA
Biological Process
    Golgi organization GO:0007030 IMP
    signal transduction GO:0007165 TAS
    Rho protein signal transduction GO:0007266 IMP
    nervous system development GO:0007399 IEA
    positive regulation of fibroblast migration GO:0010763 HDA
    lamellipodium assembly GO:0030032 IBA
    lamellipodium assembly GO:0030032 ISS
    positive regulation of apoptotic process GO:0043065 IMP
    positive regulation of GTPase activity GO:0043547 HMP
    positive regulation of GTPase activity GO:0043547 IDA
    ephrin receptor signaling pathway GO:0048013 TAS
    focal adhesion assembly GO:0048041 HDA
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 HMP
    negative regulation of microtubule nucleation GO:1905833 IMP
    positive regulation of lamellipodium morphogenesis GO:2000394 HMP
Subcellular Localization
    ruffle GO:0001726 IEA
    cytoplasm GO:0005737 IBA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 TAS
    focal adhesion GO:0005925 HDA
    cell cortex GO:0005938 IEA
    lamellipodium GO:0030027 IBA
    lamellipodium GO:0030027 ISS
    protein-containing complex GO:0032991 IDA
    neuron projection GO:0043005 IDA
    neuronal cell body GO:0043025 IDA
    mitotic spindle pole GO:0097431 IDA
    postsynapse GO:0098794 ISS
 Experiment description of studies that identified ARHGEF7 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for ARHGEF7
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 SLC9A3R2 9351
Affinity Capture-MS Homo sapiens
3 Mbp 17196
Protein-peptide Mus musculus
4 TPR 7175
Co-fractionation Homo sapiens
5 FBXW7  
Affinity Capture-MS Homo sapiens
6 PRRG4  
Reconstituted Complex Homo sapiens
7 MECP2 4204
Affinity Capture-MS Homo sapiens
8 PFDN1 5201
Affinity Capture-MS Homo sapiens
9 SNX27 81609
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
10 TRAPPC4 51399
Affinity Capture-MS Homo sapiens
11 CBLB 868
Co-crystal Structure Homo sapiens
12 ZNHIT1  
Affinity Capture-MS Homo sapiens
13 TPP2 7174
Affinity Capture-MS Homo sapiens
14 NCK1 4690
Affinity Capture-MS Homo sapiens
15 SHANK3 85358
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
16 TIMM13 26517
Affinity Capture-MS Homo sapiens
17 GRB2 2885
Affinity Capture-MS Homo sapiens
18 ICAM1 3383
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 KPNA1 3836
Affinity Capture-MS Homo sapiens
20 CDC42 998
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
21 MED4 29079
Affinity Capture-MS Homo sapiens
22 PPM1E  
Reconstituted Complex Homo sapiens
23 PAK1 5058
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 PAK3  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
25 KLHL1  
Affinity Capture-MS Homo sapiens
26 SHANK2 22941
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
27 ZYX 7791
Affinity Capture-MS Homo sapiens
28 GIT2 9815
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 PAK2 5062
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 SKP1 6500
Affinity Capture-MS Homo sapiens
31 TRIO 7204
Affinity Capture-MS Homo sapiens
32 SHANK1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
33 TUBB3 10381
Two-hybrid Homo sapiens
34 TRAPPC2L 51693
Affinity Capture-MS Homo sapiens
35 EGFR 1956
Two-hybrid Homo sapiens
PCA Homo sapiens
36 CALM1 801
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
37 PARK2  
Affinity Capture-MS Homo sapiens
38 C1orf106  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 ADAM15 8751
Reconstituted Complex Homo sapiens
40 DDX58 23586
Affinity Capture-RNA Homo sapiens
41 YWHAE 7531
Affinity Capture-MS Homo sapiens
42 VHL  
Two-hybrid Homo sapiens
43 LPXN 9404
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 NCK2 8440
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 CBL 867
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
46 NCKIPSD 51517
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 PYGM 5837
Affinity Capture-MS Homo sapiens
48 TMEM154  
Affinity Capture-MS Homo sapiens
49 C12orf49  
Negative Genetic Homo sapiens
50 CAMK4 814
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 TAX1BP3 30851
Affinity Capture-MS Homo sapiens
52 MYL6B 140465
Affinity Capture-MS Homo sapiens
53 GIT1 28964
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 REPS1 85021
Affinity Capture-MS Homo sapiens
55 LRRK2 120892
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
56 ZBTB33  
Affinity Capture-MS Homo sapiens
57 ACOX3 8310
Affinity Capture-MS Homo sapiens
58 NTRK1 4914
Affinity Capture-MS Homo sapiens
59 EXOC1 55763
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 PXN 5829
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
61 SYNC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 C20orf27 54976
Affinity Capture-MS Homo sapiens
63 ERLIN1 10613
Affinity Capture-MS Homo sapiens
64 Zbtb48  
Affinity Capture-MS Mus musculus
65 DNPEP 23549
Affinity Capture-MS Homo sapiens
66 KDM2A  
Co-fractionation Homo sapiens
67 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 MEOX2  
Two-hybrid Homo sapiens
69 CENPQ  
Affinity Capture-MS Homo sapiens
70 SCRIB 23513
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 ARHGEF6 9459
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 FBXO28  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 PSMB6 5694
Affinity Capture-MS Homo sapiens
74 RAC1 5879
Affinity Capture-MS Homo sapiens
75 HSPB1 3315
Two-hybrid Homo sapiens
76 SNRPF 6636
Affinity Capture-MS Homo sapiens
77 ATPIF1 93974
Affinity Capture-MS Homo sapiens
78 RFX1  
Affinity Capture-MS Homo sapiens
79 LATS1  
Affinity Capture-Western Homo sapiens
80 ITCH 83737
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
81 SLC33A1 9197
Affinity Capture-MS Homo sapiens
82 KLHL2 11275
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 POTEB3 102724631
Affinity Capture-MS Homo sapiens
84 PPM1F 9647
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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