Gene description for ARHGEF6
Gene name Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6
Gene symbol ARHGEF6
Other names/aliases COOL2
Cool-2
MRX46
PIXA
alpha-PIX
alphaPIX
Species Homo sapiens
 Database cross references - ARHGEF6
ExoCarta ExoCarta_9459
Entrez Gene 9459
HGNC 685
MIM 300267
UniProt Q15052  
 ARHGEF6 identified in exosomes derived from the following tissue/cell type
Thymus 23844026    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for ARHGEF6
Molecular Function
    protein binding GO:0005515 IPI
    Rho guanyl-nucleotide exchange factor activity GO:0005089 NAS
    GTPase activator activity GO:0005096 NAS
Biological Process
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    apoptotic process GO:0006915 NAS
    cell junction assembly GO:0034329 TAS
    positive regulation of apoptotic process GO:0043065 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
    JNK cascade GO:0007254 NAS
    lamellipodium assembly GO:0030032 ISS
    apoptotic signaling pathway GO:0097190 TAS
    positive regulation of GTPase activity GO:0043547 NAS
Subcellular Localization
    intracellular GO:0005622 IC
    cell-cell junction GO:0005911 IEA
    lamellipodium GO:0030027 ISS
    cytosol GO:0005829 TAS
 Experiment description of studies that identified ARHGEF6 in exosomes
1
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 193
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
PHB
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 194
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 195
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ARHGEF6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GIT1 28964
Affinity Capture-Western Homo sapiens
2 PAK1  
Invivo Homo sapiens
3 PAK3  
Invivo Homo sapiens
4 PKLR 5313
Affinity Capture-Western Homo sapiens
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