Gene description for PPP1R9B
Gene name protein phosphatase 1, regulatory subunit 9B
Gene symbol PPP1R9B
Other names/aliases PPP1R6
PPP1R9
SPINO
Spn
Species Homo sapiens
 Database cross references - PPP1R9B
ExoCarta ExoCarta_84687
Vesiclepedia VP_84687
Entrez Gene 84687
HGNC 9298
MIM 603325
UniProt Q96SB3  
 PPP1R9B identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for PPP1R9B
Molecular Function
    protein kinase activity GO:0004672 IEA
    protein phosphatase inhibitor activity GO:0004864 NAS
    protein binding GO:0005515 IPI
    protein phosphatase 1 binding GO:0008157 NAS
    kinase binding GO:0019900 IEA
    D2 dopamine receptor binding GO:0031749 IEA
    transmembrane transporter binding GO:0044325 IEA
    actin filament binding GO:0051015 IBA
Biological Process
    regulation of cell growth by extracellular stimulus GO:0001560 TAS
    response to amphetamine GO:0001975 IEA
    developmental process involved in reproduction GO:0003006 IEA
    actin filament organization GO:0007015 IBA
    regulation of exit from mitosis GO:0007096 NAS
    learning GO:0007612 IEA
    RNA splicing GO:0008380 NAS
    dendrite development GO:0016358 IEA
    cell migration GO:0016477 IMP
    calcium-mediated signaling GO:0019722 IBA
    hippocampus development GO:0021766 IEA
    cerebral cortex development GO:0021987 IEA
    actin filament depolymerization GO:0030042 IEA
    negative regulation of cell growth GO:0030308 IDA
    neuron projection development GO:0031175 IBA
    response to prostaglandin E GO:0034695 IEA
    response to nicotine GO:0035094 IEA
    response to immobilization stress GO:0035902 IEA
    regulation of cell population proliferation GO:0042127 NAS
    filopodium assembly GO:0046847 IMP
    response to steroid hormone GO:0048545 IEA
    regulation of cell cycle GO:0051726 TAS
    male mating behavior GO:0060179 IEA
    reproductive system development GO:0061458 IEA
    cellular response to morphine GO:0071315 ISS
    cellular response to epidermal growth factor stimulus GO:0071364 IEA
    cellular response to estradiol stimulus GO:0071392 IEA
    cellular response to xenobiotic stimulus GO:0071466 IEA
    regulation of postsynapse assembly GO:0150052 IEA
    cellular response to peptide GO:1901653 IEA
    positive regulation of protein localization to plasma membrane GO:1903078 IEA
    protein localization to actin cytoskeleton GO:1903119 IEA
    positive regulation of protein localization to actin cortical patch GO:1904372 IEA
    response to kainic acid GO:1904373 IEA
    response to L-phenylalanine derivative GO:1904386 IEA
    protein localization to cell periphery GO:1990778 IEA
    regulation of opioid receptor signaling pathway GO:2000474 ISS
Subcellular Localization
    protein phosphatase type 1 complex GO:0000164 TAS
    nucleoplasm GO:0005654 IMP
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    plasma membrane GO:0005886 IDA
    adherens junction GO:0005912 IEA
    postsynaptic density GO:0014069 IBA
    actin cytoskeleton GO:0015629 IBA
    lamellipodium GO:0030027 IDA
    filopodium GO:0030175 IDA
    dendrite GO:0030425 IBA
    growth cone GO:0030426 IEA
    cortical actin cytoskeleton GO:0030864 IEA
    ruffle membrane GO:0032587 IDA
    neuronal cell body GO:0043025 IEA
    dendritic spine neck GO:0044326 IEA
    dendritic spine head GO:0044327 IEA
    spine apparatus GO:0097444 IEA
    extrinsic component of postsynaptic membrane GO:0098890 IEA
    glutamatergic synapse GO:0098978 IEA
    cytoplasmic side of dendritic spine plasma membrane GO:1990780 IEA
 Experiment description of studies that identified PPP1R9B in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PPP1R9B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ARHGEF25 115557
Affinity Capture-MS Homo sapiens
2 ECT2L  
Affinity Capture-MS Homo sapiens
3 H1FNT  
Affinity Capture-MS Homo sapiens
4 PPP1CB 5500
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
5 PPP1R3A  
Affinity Capture-MS Homo sapiens
6 ADRA2A  
Reconstituted Complex Homo sapiens
7 SHC1 6464
Affinity Capture-MS Homo sapiens
8 SNX33 257364
Affinity Capture-MS Homo sapiens
9 NFATC1 4772
Affinity Capture-MS Homo sapiens
10 Calml3  
Affinity Capture-MS Mus musculus
11 DBN1 1627
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 BRCA1 672
Affinity Capture-MS Homo sapiens
13 CAT 847
Cross-Linking-MS (XL-MS) Homo sapiens
14 KIF20A 10112
Affinity Capture-MS Homo sapiens
15 SYDE2  
Affinity Capture-MS Homo sapiens
16 Pik3r2  
Affinity Capture-MS Mus musculus
17 ANKRD28 23243
Proximity Label-MS Homo sapiens
18 PPP1CC 5501
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
19 CAPZB 832
Affinity Capture-MS Homo sapiens
20 WDR48 57599
Affinity Capture-MS Homo sapiens
21 RDX 5962
Proximity Label-MS Homo sapiens
22 KIF23 9493
Affinity Capture-MS Homo sapiens
23 IQGAP1 8826
Affinity Capture-MS Homo sapiens
24 CALD1 800
Affinity Capture-MS Homo sapiens
25 CALM3 808
Affinity Capture-MS Homo sapiens
26 TIAM1  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
27 DNAJB1 3337
Affinity Capture-MS Homo sapiens
28 Flot2 14252
Affinity Capture-MS Mus musculus
29 RASGRF1  
Affinity Capture-Western Homo sapiens
30 ATG16L1 55054
Affinity Capture-MS Homo sapiens
31 CCDC130  
Affinity Capture-MS Homo sapiens
32 SNX27 81609
Affinity Capture-MS Homo sapiens
33 ARHGAP9 64333
Affinity Capture-MS Homo sapiens
34 KIF14 9928
Affinity Capture-MS Homo sapiens
35 ARHGEF11 9826
Affinity Capture-MS Homo sapiens
36 CACNB3  
Affinity Capture-MS Homo sapiens
37 RB1 5925
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
38 KRT8 3856
Proximity Label-MS Homo sapiens
39 PPP1CA 5499
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
40 VAV1 7409
Affinity Capture-MS Homo sapiens
41 MAPRE1 22919
Affinity Capture-MS Homo sapiens
42 MAPK8IP1 9479
Affinity Capture-MS Homo sapiens
43 CHMP4B 128866
Affinity Capture-MS Homo sapiens
44 MYH9 4627
Affinity Capture-MS Homo sapiens
45 CGREF1 10669
Affinity Capture-MS Homo sapiens
46 ACTN4 81
Affinity Capture-MS Homo sapiens
47 MRPL51 51258
Affinity Capture-MS Homo sapiens
48 ACTB 60
Proximity Label-MS Homo sapiens
49 ARHGEF6 9459
Affinity Capture-MS Homo sapiens
50 COPS3 8533
Affinity Capture-MS Homo sapiens
51 Coro1c 23790
Affinity Capture-MS Mus musculus
52 ADRA2B  
Reconstituted Complex Homo sapiens
53 CDIPT 10423
Affinity Capture-MS Homo sapiens
54 RBL1  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
55 ANLN 54443
Affinity Capture-MS Homo sapiens
56 RING1 6015
Cross-Linking-MS (XL-MS) Homo sapiens
57 LIMA1 51474
Affinity Capture-MS Homo sapiens
58 BRF1  
Affinity Capture-MS Homo sapiens
59 CCM2 83605
Affinity Capture-MS Homo sapiens
60 Myh9 17886
Affinity Capture-MS Mus musculus
61 ECT2 1894
Affinity Capture-MS Homo sapiens
62 RLIM 51132
Affinity Capture-MS Homo sapiens
63 C11orf49  
Affinity Capture-MS Homo sapiens
64 CUL3 8452
Affinity Capture-MS Homo sapiens
65 YWHAZ 7534
Cross-Linking-MS (XL-MS) Homo sapiens
66 CTTN 2017
Affinity Capture-MS Homo sapiens
67 MYO19  
Affinity Capture-MS Homo sapiens
68 SUZ12  
Affinity Capture-MS Homo sapiens
69 Ect2  
Affinity Capture-MS Mus musculus
70 ZC3H10  
Affinity Capture-MS Homo sapiens
71 CDC42 998
Affinity Capture-MS Homo sapiens
72 CYP51A1 1595
Affinity Capture-MS Homo sapiens
73 ARHGAP40  
Affinity Capture-MS Homo sapiens
74 SYNPO 11346
Affinity Capture-MS Homo sapiens
75 MYC  
Affinity Capture-MS Homo sapiens
76 FDPS 2224
Affinity Capture-MS Homo sapiens
77 RBL2 5934
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
78 RPS14 6208
Affinity Capture-MS Homo sapiens
79 GOLGA1  
Proximity Label-MS Homo sapiens
80 DCX  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
81 RNF2  
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
82 PCM1 5108
Proximity Label-MS Homo sapiens
83 ZC2HC1C  
Affinity Capture-MS Homo sapiens
84 DRD2  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
85 CCDC68  
Affinity Capture-MS Homo sapiens
86 CCND2  
Affinity Capture-MS Homo sapiens
87 CSNK1A1L 122011
Affinity Capture-MS Homo sapiens
88 USP46 64854
Affinity Capture-MS Homo sapiens
89 UBA5 79876
Affinity Capture-MS Homo sapiens
90 NMI  
Affinity Capture-MS Homo sapiens
91 ADRA2C  
Reconstituted Complex Homo sapiens
92 EZR 7430
Proximity Label-MS Homo sapiens
93 Myo1c 17913
Affinity Capture-MS Mus musculus
94 PDHA1 5160
Affinity Capture-MS Homo sapiens
95 POP4 10775
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 CAPZA2 830
Affinity Capture-MS Homo sapiens
97 CYTH4  
Affinity Capture-MS Homo sapiens
98 SALL1 6299
Affinity Capture-MS Homo sapiens
99 DDX58 23586
Affinity Capture-RNA Homo sapiens
100 ACTA1 58
Reconstituted Complex Homo sapiens
101 PPP1R2 5504
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
102 ARHGAP25  
Affinity Capture-MS Homo sapiens
103 KXD1 79036
Affinity Capture-MS Homo sapiens
104 FLNA 2316
Affinity Capture-MS Homo sapiens
105 RPL8 6132
Affinity Capture-MS Homo sapiens
106 SOS2 6655
Affinity Capture-MS Homo sapiens
107 CDKN2A 1029
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
108 CASQ2 845
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PPP1R9B is involved
No pathways found





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