Gene description for ARHGEF11
Gene name Rho guanine nucleotide exchange factor (GEF) 11
Gene symbol ARHGEF11
Other names/aliases GTRAP48
PDZ-RHOGEF
Species Homo sapiens
 Database cross references - ARHGEF11
ExoCarta ExoCarta_9826
Vesiclepedia VP_9826
Entrez Gene 9826
HGNC 14580
MIM 605708
UniProt O15085  
 ARHGEF11 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for ARHGEF11
Molecular Function
    G protein-coupled receptor binding GO:0001664 IBA
    G protein-coupled receptor binding GO:0001664 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 IBA
    guanyl-nucleotide exchange factor activity GO:0005085 TAS
    GTPase activator activity GO:0005096 IEA
    protein binding GO:0005515 IPI
Biological Process
    regulation of cell growth GO:0001558 NAS
    striated muscle contraction GO:0006941 NAS
    G protein-coupled receptor signaling pathway GO:0007186 IBA
    G protein-coupled receptor signaling pathway GO:0007186 IDA
    G protein-coupled receptor signaling pathway GO:0007186 TAS
    Rho protein signal transduction GO:0007266 IDA
    establishment of cell polarity GO:0030010 NAS
    actin cytoskeleton organization GO:0030036 NAS
    positive regulation of DNA-templated transcription GO:0045893 IDA
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified ARHGEF11 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ARHGEF11
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PTPRF 5792
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
2 MYO5A 4644
Affinity Capture-MS Homo sapiens
3 ARRB2 409
Affinity Capture-MS Homo sapiens
4 PLXNB2 23654
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 GNA12 2768
Affinity Capture-Western Homo sapiens
Phenotypic Enhancement Homo sapiens
6 MPRIP 23164
Affinity Capture-MS Homo sapiens
7 GSK3B 2932
Affinity Capture-Western Homo sapiens
8 RHOC 389
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 TFB1M  
Affinity Capture-MS Homo sapiens
10 PFN1 5216
Proximity Label-MS Homo sapiens
11 ISCA2 122961
Affinity Capture-MS Homo sapiens
12 KRT19 3880
Proximity Label-MS Homo sapiens
13 S100A2 6273
Affinity Capture-MS Homo sapiens
14 PLXNB3 5365
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
15 NOA1  
Affinity Capture-MS Homo sapiens
16 POTEE 445582
Affinity Capture-MS Homo sapiens
17 PPP1R9B 84687
Affinity Capture-MS Homo sapiens
18 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
19 PLXNB1 5364
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
20 HOOK3 84376
Proximity Label-MS Homo sapiens
21 PLEKHG4B 153478
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 FAM124B  
Affinity Capture-MS Homo sapiens
23 S100A13 6284
Affinity Capture-MS Homo sapiens
24 TMOD1  
Affinity Capture-MS Homo sapiens
25 SSBP1 6742
Affinity Capture-MS Homo sapiens
26 CDC42 998
Two-hybrid Homo sapiens
27 GSK3A 2931
Affinity Capture-Western Homo sapiens
28 TIGD3  
Affinity Capture-MS Homo sapiens
29 MTX2 10651
Affinity Capture-MS Homo sapiens
30 TRMT61A 115708
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 LONP2 83752
Affinity Capture-MS Homo sapiens
32 INA 9118
Affinity Capture-MS Homo sapiens
33 PAK4 10298
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 NTRK1 4914
Affinity Capture-MS Homo sapiens
35 TMOD2 29767
Affinity Capture-MS Homo sapiens
36 PPIE 10450
Affinity Capture-MS Homo sapiens
37 COMMD9 29099
Affinity Capture-MS Homo sapiens
38 DTX2 113878
Proximity Label-MS Homo sapiens
39 WDR59 79726
Affinity Capture-MS Homo sapiens
40 CGN  
Affinity Capture-MS Homo sapiens
41 GNA13 10672
Affinity Capture-Western Homo sapiens
Phenotypic Enhancement Homo sapiens
42 RHOB 388
Proximity Label-MS Homo sapiens
43 Nedd1  
Affinity Capture-MS Mus musculus
44 ARHGEF12 23365
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 PAK7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 RHOA 387
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
FRET Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
47 Sept9  
Affinity Capture-MS Mus musculus
48 PDF  
Affinity Capture-MS Homo sapiens
49 ARHGEF40 55701
Affinity Capture-MS Homo sapiens
50 ARAP2  
Co-fractionation Homo sapiens
51 RPRD1A  
Affinity Capture-MS Homo sapiens
52 MZT2B 80097
Affinity Capture-MS Homo sapiens
53 LRCH3 84859
Affinity Capture-MS Homo sapiens
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