Gene description for WDR59
Gene name WD repeat domain 59
Gene symbol WDR59
Other names/aliases FP977
Species Homo sapiens
 Database cross references - WDR59
ExoCarta ExoCarta_79726
Vesiclepedia VP_79726
Entrez Gene 79726
HGNC 25706
UniProt Q6PJI9  
 WDR59 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for WDR59
Molecular Function
    protein binding GO:0005515 IPI
    signaling adaptor activity GO:0035591 IBA
    metal ion binding GO:0046872 IEA
Biological Process
    cellular response to nutrient levels GO:0031669 IDA
    cellular response to amino acid starvation GO:0034198 IBA
    cellular response to amino acid starvation GO:0034198 IMP
    negative regulation of TORC1 signaling GO:1904262 NAS
    positive regulation of TORC1 signaling GO:1904263 IBA
    positive regulation of TORC1 signaling GO:1904263 IDA
    positive regulation of TORC1 signaling GO:1904263 NAS
Subcellular Localization
    lysosomal membrane GO:0005765 IDA
    lysosomal membrane GO:0005765 TAS
    vacuolar membrane GO:0005774 IBA
    cytosol GO:0005829 IDA
    Seh1-associated complex GO:0035859 IBA
    intracellular membrane-bounded organelle GO:0043231 IDA
    GATOR2 complex GO:0061700 IDA
    GATOR2 complex GO:0061700 NAS
 Experiment description of studies that identified WDR59 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for WDR59
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEC22B 9554
Affinity Capture-MS Homo sapiens
2 NPRL2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 GATSL2  
Affinity Capture-Western Homo sapiens
4 FAM167A  
Affinity Capture-MS Homo sapiens
5 PAEP  
Affinity Capture-MS Homo sapiens
6 Sesn2  
Affinity Capture-MS Mus musculus
7 CRYL1 51084
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 HDHD1  
Affinity Capture-MS Homo sapiens
9 LAMP2 3920
Proximity Label-MS Homo sapiens
Co-localization Homo sapiens
10 GRB10 2887
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 LOXL4 84171
Affinity Capture-MS Homo sapiens
12 HDAC3 8841
Affinity Capture-MS Homo sapiens
13 TRMU  
Affinity Capture-MS Homo sapiens
14 TBL1XR1 79718
Affinity Capture-MS Homo sapiens
15 D2HGDH 728294
Affinity Capture-MS Homo sapiens
16 DUSP14 11072
Affinity Capture-MS Homo sapiens
17 THTPA  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 CREB1  
Affinity Capture-MS Homo sapiens
19 GUSBP5  
Affinity Capture-MS Homo sapiens
20 FAF1 11124
Affinity Capture-MS Homo sapiens
21 SEH1L 81929
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
22 TUBA1A 7846
Affinity Capture-MS Homo sapiens
23 KPTN  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
24 NPAS1  
Affinity Capture-MS Homo sapiens
25 WDR5 11091
Affinity Capture-Western Homo sapiens
26 FBXW5  
Affinity Capture-MS Homo sapiens
27 DHDH  
Affinity Capture-MS Homo sapiens
28 KIF14 9928
Affinity Capture-MS Homo sapiens
29 ARHGEF11 9826
Affinity Capture-MS Homo sapiens
30 ITFG2  
Affinity Capture-Western Homo sapiens
31 CCT2 10576
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 SULT1C4  
Affinity Capture-MS Homo sapiens
33 ECT2 1894
Affinity Capture-MS Homo sapiens
34 BRMS1 25855
Affinity Capture-MS Homo sapiens
35 NCOR2  
Affinity Capture-MS Homo sapiens
36 SZT2 23334
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
37 GATSL3 652968
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
38 TRMT61A 115708
Affinity Capture-MS Homo sapiens
39 KIAA1683  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 NTRK1 4914
Affinity Capture-MS Homo sapiens
41 SESN3  
Reconstituted Complex Homo sapiens
42 CLEC11A 6320
Affinity Capture-MS Homo sapiens
43 ANKRD13A 88455
Affinity Capture-MS Homo sapiens
44 NRF1 4899
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 UEVLD 55293
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 NPRL3  
Affinity Capture-Western Homo sapiens
47 SEC13 6396
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 PITHD1 57095
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 LAMP1 3916
Affinity Capture-Western Homo sapiens
50 PTH2R  
Affinity Capture-MS Homo sapiens
51 SKP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 MAP2K2 5605
Affinity Capture-MS Homo sapiens
53 WDR24  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
54 RPA3 6119
Proximity Label-MS Homo sapiens
55 SOHLH1  
Affinity Capture-MS Homo sapiens
56 RAB9A 9367
Proximity Label-MS Homo sapiens
57 TBL1X 6907
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 HDLBP 3069
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 SF3B3 23450
Affinity Capture-MS Homo sapiens
60 TXNDC5 81567
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 RBBP5 5929
Affinity Capture-MS Homo sapiens
62 CBWD1 55871
Affinity Capture-MS Homo sapiens
63 RAE1 8480
Affinity Capture-MS Homo sapiens
64 CRNN 49860
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 NUP54 53371
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 FASN 2194
Positive Genetic Homo sapiens
67 LAMTOR1 55004
Proximity Label-MS Homo sapiens
68 AVIL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 SIN3A  
Affinity Capture-MS Homo sapiens
70 DOCK5 80005
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 TSPYL6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 BLOC1S5-TXNDC5  
Affinity Capture-MS Homo sapiens
73 DNAJA2 10294
Affinity Capture-MS Homo sapiens
74 CTAG1B  
Affinity Capture-MS Homo sapiens
75 DEPDC5  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
76 EPB41L2 2037
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 OLFM4 10562
Affinity Capture-MS Homo sapiens
78 DDX58 23586
Affinity Capture-RNA Homo sapiens
79 CCT7 10574
Affinity Capture-MS Homo sapiens
80 ANO1 55107
Affinity Capture-MS Homo sapiens
81 MIOS 54468
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
82 GLUL 2752
Affinity Capture-MS Homo sapiens
83 INF2 64423
Affinity Capture-MS Homo sapiens
84 C12orf66  
Affinity Capture-Western Homo sapiens
85 C12orf74  
Affinity Capture-MS Homo sapiens
86 HSF1  
Affinity Capture-MS Homo sapiens
87 RAB7A 7879
Proximity Label-MS Homo sapiens
88 RANGRF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 HSD17B7P2  
Affinity Capture-MS Homo sapiens
90 UPP1 7378
Affinity Capture-MS Homo sapiens
91 VIM 7431
Two-hybrid Homo sapiens
92 SESN2 83667
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
93 EP300 2033
Two-hybrid Homo sapiens
View the network image/svg+xml



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