Gene description for ANO1
Gene name anoctamin 1, calcium activated chloride channel
Gene symbol ANO1
Other names/aliases DOG1
ORAOV2
TAOS2
TMEM16A
Species Homo sapiens
 Database cross references - ANO1
ExoCarta ExoCarta_55107
Entrez Gene 55107
HGNC 21625
MIM 610108
UniProt Q5XXA6  
 ANO1 identified in exosomes derived from the following tissue/cell type
Saliva 19199708    
 Gene ontology annotations for ANO1
Molecular Function
    protein binding GO:0005515 IPI
    protein homodimerization activity GO:0042803 IEA
    intracellular calcium activated chloride channel activity GO:0005229 ISS
    calcium activated cation channel activity GO:0005227 IDA
    protein heterodimerization activity GO:0046982 IEA
Biological Process
    transmembrane transport GO:0055085 TAS
    ion transmembrane transport GO:0034220 TAS
    cation transport GO:0006812 IDA
    chloride transmembrane transport GO:1902476 IEA
    regulation of membrane potential GO:0042391 IEA
    cation transmembrane transport GO:0098655 IDA
    multicellular organismal development GO:0007275 IEA
    chloride transport GO:0006821 ISS
Subcellular Localization
    cytoplasm GO:0005737 IEA
    apical plasma membrane GO:0016324 IEA
    extracellular exosome GO:0070062 IDA
    plasma membrane GO:0005886 TAS
    external side of plasma membrane GO:0009897 IEA
    chloride channel complex GO:0034707 IEA
 Experiment description of studies that identified ANO1 in exosomes
1
Experiment ID 66
ISEV standards
IEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD63|CD81
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for ANO1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which ANO1 is involved
PathwayEvidenceSource
Stimuli-sensing channels TAS Reactome





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