Gene description for NUP54
Gene name nucleoporin 54kDa
Gene symbol NUP54
Other names/aliases -
Species Homo sapiens
 Database cross references - NUP54
ExoCarta ExoCarta_53371
Vesiclepedia VP_53371
Entrez Gene 53371
HGNC 17359
MIM 607607
UniProt Q7Z3B4  
 NUP54 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for NUP54
Molecular Function
    protein binding GO:0005515 IPI
    nucleocytoplasmic transporter activity GO:0005487 IEA
Biological Process
    mRNA transport GO:0051028 IEA
    transmembrane transport GO:0055085 TAS
    mitotic cell cycle GO:0000278 TAS
    small molecule metabolic process GO:0044281 TAS
    hexose transport GO:0008645 TAS
    cellular response to heat GO:0034605 TAS
    cytokine-mediated signaling pathway GO:0019221 TAS
    post-translational protein modification GO:0043687 TAS
    mitotic nuclear envelope disassembly GO:0007077 TAS
    cellular protein metabolic process GO:0044267 TAS
    nucleocytoplasmic transport GO:0006913 IEA
    carbohydrate metabolic process GO:0005975 TAS
    protein targeting GO:0006605 IEA
    protein sumoylation GO:0016925 TAS
    gene expression GO:0010467 TAS
    regulation of cellular response to heat GO:1900034 TAS
    viral transcription GO:0019083 TAS
    regulation of glucose transport GO:0010827 TAS
    viral life cycle GO:0019058 TAS
    viral process GO:0016032 TAS
    glucose transport GO:0015758 TAS
Subcellular Localization
    nuclear pore GO:0005643 IEA
    cytoplasm GO:0005737 TAS
    nuclear membrane GO:0031965 IEA
    nucleoplasm GO:0005654 TAS
    nuclear envelope GO:0005635 TAS
 Experiment description of studies that identified NUP54 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NUP54
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DIPA  
Two-hybrid Homo sapiens
2 NUP62 23636
Two-hybrid Homo sapiens
3 KPNB1 3837
Reconstituted Complex Homo sapiens
4 AP2B1 163
Two-hybrid Homo sapiens
5 NUTF2 10204
Reconstituted Complex Homo sapiens
6 KPNA1 3836
Reconstituted Complex Homo sapiens
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