Gene description for UPP1
Gene name uridine phosphorylase 1
Gene symbol UPP1
Other names/aliases UDRPASE
UP
UPASE
UPP
Species Homo sapiens
 Database cross references - UPP1
ExoCarta ExoCarta_7378
Vesiclepedia VP_7378
Entrez Gene 7378
HGNC 12576
MIM 191730
UniProt Q16831  
 UPP1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for UPP1
Molecular Function
    uridine phosphorylase activity GO:0004850 IBA
    uridine phosphorylase activity GO:0004850 IDA
    thymidine phosphorylase activity GO:0009032 IEA
    identical protein binding GO:0042802 IPI
    deoxyuridine phosphorylase activity GO:0047847 IEA
Biological Process
    nucleobase-containing compound metabolic process GO:0006139 TAS
    uridine catabolic process GO:0006218 IBA
    uridine catabolic process GO:0006218 IDA
    CMP catabolic process GO:0006248 IEA
    dCMP catabolic process GO:0006249 IEA
    cellular response to glucose starvation GO:0042149 IEA
    UMP salvage GO:0044206 IEA
    UMP catabolic process GO:0046050 IEA
    dTMP catabolic process GO:0046074 IEA
    dUMP catabolic process GO:0046079 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified UPP1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
EM
Biophysical techniques
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 191
MISEV standards
Biophysical techniques
Alix|CD81|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for UPP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ANAPC7 51434
Affinity Capture-MS Homo sapiens
2 MPST 4357
Co-fractionation Homo sapiens
3 HERC1 8925
Affinity Capture-MS Homo sapiens
4 KIAA0100  
Affinity Capture-MS Homo sapiens
5 UCK2 7371
Co-fractionation Homo sapiens
6 PRR14L 253143
Affinity Capture-MS Homo sapiens
7 FHL1 2273
Affinity Capture-MS Homo sapiens
8 RIC8B 55188
Affinity Capture-MS Homo sapiens
9 WBP2 23558
Co-fractionation Homo sapiens
10 ZNFX1 57169
Affinity Capture-MS Homo sapiens
11 PPP2R2B 5521
Affinity Capture-MS Homo sapiens
12 GTF3C5 9328
Affinity Capture-MS Homo sapiens
13 CUTA 51596
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 USP34 9736
Affinity Capture-MS Homo sapiens
15 A2ML1 144568
Affinity Capture-MS Homo sapiens
16 TBC1D4  
Affinity Capture-MS Homo sapiens
17 ALMS1  
Affinity Capture-MS Homo sapiens
18 CTPS2 56474
Affinity Capture-MS Homo sapiens
19 PPIP5K1 9677
Affinity Capture-MS Homo sapiens
20 UBR4 23352
Affinity Capture-MS Homo sapiens
21 KCMF1 56888
Affinity Capture-MS Homo sapiens
22 EBNA-LP  
Affinity Capture-MS
23 RPAP1  
Affinity Capture-MS Homo sapiens
24 TUBB8 347688
Affinity Capture-MS Homo sapiens
25 WDR45B 56270
Co-fractionation Homo sapiens
26 RICTOR 253260
Affinity Capture-MS Homo sapiens
27 MARCH7  
Affinity Capture-MS Homo sapiens
28 SETX  
Affinity Capture-MS Homo sapiens
29 IRAK1  
Affinity Capture-MS Homo sapiens
30 GPD1L 23171
Co-fractionation Homo sapiens
31 IGHG2 3501
Affinity Capture-MS Homo sapiens
32 TRIM32 22954
Affinity Capture-MS Homo sapiens
33 FNDC5  
Affinity Capture-MS Homo sapiens
34 TUBA1A 7846
Affinity Capture-MS Homo sapiens
35 FDFT1 2222
Affinity Capture-MS Homo sapiens
36 ARMCX5  
Affinity Capture-MS Homo sapiens
37 RPE  
Co-fractionation Homo sapiens
38 ZNF195  
Affinity Capture-MS Homo sapiens
39 BRCA2 675
Affinity Capture-MS Homo sapiens
40 MTUS1  
Affinity Capture-MS Homo sapiens
41 TANGO6 79613
Affinity Capture-MS Homo sapiens
42 PPOX 5498
Affinity Capture-MS Homo sapiens
43 IGHG1 3500
Affinity Capture-MS Homo sapiens
44 MCM2 4171
Affinity Capture-MS Homo sapiens
45 IGHG4 3503
Affinity Capture-MS Homo sapiens
46 WDR59 79726
Affinity Capture-MS Homo sapiens
47 RNF219  
Affinity Capture-MS Homo sapiens
48 SSUH2  
Affinity Capture-MS Homo sapiens
49 BBS2 583
Affinity Capture-MS Homo sapiens
50 CUL7 9820
Affinity Capture-MS Homo sapiens
51 LRRC45  
Affinity Capture-MS Homo sapiens
52 OR2A4 79541
Affinity Capture-MS Homo sapiens
53 RECQL4  
Affinity Capture-MS Homo sapiens
54 VIM 7431
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
55 CCR1  
Affinity Capture-MS Homo sapiens
56 HAUS6  
Affinity Capture-MS Homo sapiens
57 GPAT2  
Affinity Capture-MS Homo sapiens
58 VPS18 57617
Affinity Capture-MS Homo sapiens
59 IGKC 3514
Affinity Capture-MS Homo sapiens
60 PASK  
Affinity Capture-MS Homo sapiens
61 YRDC 79693
Co-fractionation Homo sapiens
62 Stmn1 16765
Affinity Capture-MS Mus musculus
63 FUK 197258
Affinity Capture-MS Homo sapiens
64 UBE2D1 7321
Co-fractionation Homo sapiens
View the network image/svg+xml



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