Gene description for GPD1L
Gene name glycerol-3-phosphate dehydrogenase 1-like
Gene symbol GPD1L
Other names/aliases GPD1-L
Species Homo sapiens
 Database cross references - GPD1L
ExoCarta ExoCarta_23171
Vesiclepedia VP_23171
Entrez Gene 23171
HGNC 28956
MIM 611778
UniProt Q8N335  
 GPD1L identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for GPD1L
Molecular Function
    protein binding GO:0005515 IPI
    sodium channel regulator activity GO:0017080 IMP
    protein homodimerization activity GO:0042803 IEA
    transmembrane transporter binding GO:0044325 IPI
    glycerol-3-phosphate dehydrogenase [NAD(P)+] activity GO:0047952 IBA
    NAD binding GO:0051287 IEA
    glycerol-3-phosphate dehydrogenase (NAD+) activity GO:0141152 IEA
Biological Process
    regulation of heart rate GO:0002027 IMP
    carbohydrate metabolic process GO:0005975 IEA
    NADH oxidation GO:0006116 IBA
    positive regulation of sodium ion transport GO:0010765 IMP
    NAD metabolic process GO:0019674 IMP
    glycerol-3-phosphate catabolic process GO:0046168 IEA
    regulation of ventricular cardiac muscle cell membrane depolarization GO:0060373 IMP
    ventricular cardiac muscle cell action potential GO:0086005 IMP
    positive regulation of protein localization to cell surface GO:2000010 IMP
Subcellular Localization
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified GPD1L in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GPD1L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TGOLN2 10618
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 SPANXN5  
Affinity Capture-MS Homo sapiens
3 GNPNAT1 64841
Co-fractionation Homo sapiens
4 MYH7B  
Co-fractionation Homo sapiens
5 PALM2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 ART3  
Affinity Capture-MS Homo sapiens
8 TMEM9 252839
Affinity Capture-MS Homo sapiens
9 AIFM1 9131
Affinity Capture-MS Homo sapiens
10 GPR17 2840
Affinity Capture-MS Homo sapiens
11 SSR2  
Affinity Capture-MS Homo sapiens
12 FAS 355
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 HIST1H2BC 8347
Cross-Linking-MS (XL-MS) Homo sapiens
14 HCST  
Affinity Capture-MS Homo sapiens
15 BSG 682
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 RPA2 6118
Proximity Label-MS Homo sapiens
17 CST8  
Affinity Capture-MS Homo sapiens
18 HIST1H1E 3008
Affinity Capture-MS Homo sapiens
19 KLRC4  
Affinity Capture-MS Homo sapiens
20 ALDOA 226
Co-fractionation Homo sapiens
21 GPD1 2819
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 LAMP2 3920
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 GPT2  
Co-fractionation Homo sapiens
24 CCL5 6352
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
26 FCER1A  
Affinity Capture-MS Homo sapiens
27 NDUFA4 4697
Affinity Capture-MS Homo sapiens
28 ARL6IP1 23204
Cross-Linking-MS (XL-MS) Homo sapiens
29 GOLGA8G  
Affinity Capture-MS Homo sapiens
30 CD80 941
Affinity Capture-MS Homo sapiens
31 S100A4 6275
Co-fractionation Homo sapiens
32 MICB  
Affinity Capture-MS Homo sapiens
33 GAPDH 2597
Co-fractionation Homo sapiens
34 RPA3 6119
Proximity Label-MS Homo sapiens
35 SLC3A2 6520
Affinity Capture-MS Homo sapiens
36 ALDOC 230
Co-fractionation Homo sapiens
37 GPR45  
Affinity Capture-MS Homo sapiens
38 CHODL 140578
Affinity Capture-MS Homo sapiens
39 TPI1 7167
Co-fractionation Homo sapiens
40 CKMT1A 548596
Co-fractionation Homo sapiens
41 CCL16  
Affinity Capture-MS Homo sapiens
42 ACO2 50
Co-fractionation Homo sapiens
43 FAM174A 345757
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 PYCRL 65263
Co-fractionation Homo sapiens
45 SERBP1 26135
Affinity Capture-MS Homo sapiens
46 FH 2271
Co-fractionation Homo sapiens
47 GLMP 112770
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 PGM1 5236
Co-fractionation Homo sapiens
49 CYCS 54205
Co-fractionation Homo sapiens
50 KLRC1  
Affinity Capture-MS Homo sapiens
51 ISLR 3671
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 HIST1H2BK 85236
Cross-Linking-MS (XL-MS) Homo sapiens
53 SLC22A4 6583
Affinity Capture-MS Homo sapiens
54 SLAMF8  
Affinity Capture-MS Homo sapiens
55 DKKL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 CUL4A 8451
Affinity Capture-MS Homo sapiens
57 TACSTD2 4070
Affinity Capture-MS Homo sapiens
58 PHGDH 26227
Co-fractionation Homo sapiens
59 UPP1 7378
Co-fractionation Homo sapiens
60 CKMT1B 1159
Co-fractionation Homo sapiens
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