Gene description for PPOX
Gene name protoporphyrinogen oxidase
Gene symbol PPOX
Other names/aliases PPO
V290M
VP
Species Homo sapiens
 Database cross references - PPOX
ExoCarta ExoCarta_5498
Vesiclepedia VP_5498
Entrez Gene 5498
HGNC 9280
MIM 600923
UniProt P50336  
 PPOX identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PPOX
Molecular Function
    oxygen-dependent protoporphyrinogen oxidase activity GO:0004729 IBA
    oxygen-dependent protoporphyrinogen oxidase activity GO:0004729 IDA
    flavin adenine dinucleotide binding GO:0050660 TAS
    menaquinone-dependent protoporphyrinogen oxidase activity GO:0070819 IEA
Biological Process
    porphyrin-containing compound biosynthetic process GO:0006779 IDA
    protoporphyrinogen IX biosynthetic process GO:0006782 IEA
    heme biosynthetic process GO:0006783 IBA
    heme biosynthetic process GO:0006783 IDA
    heme A biosynthetic process GO:0006784 IEA
    heme B biosynthetic process GO:0006785 IEA
    heme O biosynthetic process GO:0048034 IEA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrial inner membrane GO:0005743 IBA
    mitochondrial inner membrane GO:0005743 ISS
    mitochondrial intermembrane space GO:0005758 TAS
    mitochondrial membrane GO:0031966 IMP
 Experiment description of studies that identified PPOX in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PPOX
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HAP1  
Two-hybrid Homo sapiens
2 NIPSNAP3A  
Affinity Capture-MS Homo sapiens
3 ARSA 410
Affinity Capture-MS Homo sapiens
4 IMMT 10989
Two-hybrid Homo sapiens
5 HNRNPD 3184
Two-hybrid Homo sapiens
6 UQCRFS1 7386
Affinity Capture-MS Homo sapiens
7 TRMU  
Affinity Capture-MS Homo sapiens
8 CA6 765
Affinity Capture-MS Homo sapiens
9 RAMP2  
Affinity Capture-MS Homo sapiens
10 LDHAL6B 92483
Affinity Capture-MS Homo sapiens
11 GPR45  
Affinity Capture-MS Homo sapiens
12 AK4 205
Affinity Capture-MS Homo sapiens
13 NAA38  
Affinity Capture-MS Homo sapiens
14 MRPS2 51116
Affinity Capture-MS Homo sapiens
15 TCTN1  
Affinity Capture-MS Homo sapiens
16 KIF20A 10112
Affinity Capture-MS Homo sapiens
17 TAZ  
Affinity Capture-MS Homo sapiens
18 MRPS30 10884
Affinity Capture-MS Homo sapiens
19 UPP1 7378
Affinity Capture-MS Homo sapiens
20 PLAUR 5329
Affinity Capture-MS Homo sapiens
21 METTL20  
Affinity Capture-MS Homo sapiens
22 ACAA2 10449
Affinity Capture-MS Homo sapiens
23 MTG2  
Affinity Capture-MS Homo sapiens
24 ACSM5 54988
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 CTAG1B  
Affinity Capture-MS Homo sapiens
26 NDUFS7 374291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 BPNT1 10380
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 FAM83F 113828
Affinity Capture-MS Homo sapiens
29 GAA 2548
Affinity Capture-MS Homo sapiens
30 TRIM43  
Affinity Capture-MS Homo sapiens
31 SNX21  
Affinity Capture-MS Homo sapiens
32 FCGR3B  
Affinity Capture-MS Homo sapiens
33 KLRD1  
Affinity Capture-MS Homo sapiens
34 AMACR 23600
Affinity Capture-MS Homo sapiens
35 ADAM7  
Affinity Capture-MS Homo sapiens
36 FMOD 2331
Affinity Capture-MS Homo sapiens
37 ANTXR1 84168
Affinity Capture-MS Homo sapiens
38 APEX1 328
Affinity Capture-RNA Homo sapiens
39 KIAA1429 25962
Affinity Capture-MS Homo sapiens
40 PARK2  
Affinity Capture-MS Homo sapiens
41 CRYBA4  
Affinity Capture-MS Homo sapiens
42 IL12RB1  
Affinity Capture-MS Homo sapiens
43 NAAA  
Affinity Capture-MS Homo sapiens
44 EGFR 1956
Negative Genetic Homo sapiens
45 COQ9  
Affinity Capture-MS Homo sapiens
46 SCGB2A2  
Affinity Capture-MS Homo sapiens
47 GPR182  
Affinity Capture-MS Homo sapiens
48 DHDH  
Affinity Capture-MS Homo sapiens
49 THBS3 7059
Affinity Capture-MS Homo sapiens
50 COPS5 10987
Two-hybrid Homo sapiens
51 PCDHA12  
Affinity Capture-MS Homo sapiens
52 YARS2  
Affinity Capture-MS Homo sapiens
53 MRPS24 64951
Affinity Capture-MS Homo sapiens
54 QRSL1  
Affinity Capture-MS Homo sapiens
55 DNASE1L1 1774
Affinity Capture-MS Homo sapiens
56 NDUFA10 4705
Affinity Capture-MS Homo sapiens
57 ISLR 3671
Affinity Capture-MS Homo sapiens
58 SLC1A1 6505
Affinity Capture-MS Homo sapiens
59 NANOG  
Affinity Capture-MS Homo sapiens
60 SDF4 51150
Affinity Capture-MS Homo sapiens
61 CBWD1 55871
Affinity Capture-MS Homo sapiens
62 CLEC4E  
Affinity Capture-MS Homo sapiens
63 POU5F1  
Affinity Capture-MS Homo sapiens
64 HYPM  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PPOX is involved
PathwayEvidenceSource
Heme biosynthesis TAS Reactome
Metabolism TAS Reactome
Metabolism of porphyrins TAS Reactome





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