Experiment description of studies that identified IGHG4 in sEVs

1 Experiment ID 497 MISEV standards ✘ Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

2 Experiment ID 498 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35931686     Organism Homo sapiens Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD." Journal name Cell Death Dis Publication year 2022 Sample Chondrocytes Sample name T/C-28a2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

3 Experiment ID 21 MISEV standards ✔ EM|IEM Biophysical techniques ✔ Alix|TSG101|HSP70|CD63 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry    PubMed ID 19837982     Organism Homo sapiens Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215. Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson" Journal name MCP Publication year 2009 Sample Colorectal cancer cells Sample name LIM1215 Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.10-1.12 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [Orbitrap] Western blotting

4 Experiment ID 126 MISEV standards ✘ Biophysical techniques ✔ GAPDH Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [LTQ-FT Ultra] PubMed ID Unpublished / Not applicable Organism Homo sapiens Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim" Journal name Mesenchymal Stem Cell Therapy Publication year 2011 Sample Mesenchymal stem cells Sample name huES9.E1 Isolation/purification methods HPLC Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Antobody array Mass spectrometry

5 Experiment ID 231 MISEV standards ✘ Biophysical techniques ✔ Alix|CD63|CD9 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Lipids Methods used in the study Western blotting Mass spectrometry

6 Experiment ID 232 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

7 Experiment ID 233 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for IGHG4

Protein Interactor ExoCarta ID Identification method PubMed Species 1 HYPK 25764 Affinity Capture-MS Homo sapiens 2 CCBL1 883 Affinity Capture-MS Homo sapiens 3 CIR1   Affinity Capture-MS Homo sapiens 4 SNX27 81609 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 5 TMPO 7112 Affinity Capture-MS Homo sapiens 6 PPP2R2B 5521 Affinity Capture-MS Homo sapiens 7 SLC25A6 293 Affinity Capture-MS Homo sapiens 8 C18orf21   Affinity Capture-MS Homo sapiens 9 FANCD2   Affinity Capture-MS Homo sapiens 10 AIFM1 9131 Affinity Capture-MS Homo sapiens 11 H2AFB1   Affinity Capture-MS Homo sapiens 12 AGPAT1 10554 Affinity Capture-MS Homo sapiens 13 SCGB1D1   Affinity Capture-MS Homo sapiens 14 ATF6   Affinity Capture-MS Homo sapiens 15 NRSN1   Affinity Capture-MS Homo sapiens 16 RLIM 51132 Affinity Capture-MS Homo sapiens 17 ALDH2 217 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 18 EMC4 51234 Affinity Capture-MS Homo sapiens 19 CCR1   Affinity Capture-MS Homo sapiens 20 RAD51AP1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 21 RPS2P32   Affinity Capture-MS Homo sapiens 22 EDC3 80153 Affinity Capture-MS Homo sapiens 23 ASPDH   Affinity Capture-MS Homo sapiens 24 MORF4L1   Affinity Capture-MS Homo sapiens 25 C1orf35   Affinity Capture-MS Homo sapiens 26 CERS3   Affinity Capture-MS Homo sapiens 27 TF 7018 Affinity Capture-MS Homo sapiens 28 RHBDD1 84236 Affinity Capture-MS Homo sapiens 29 RNF115   Affinity Capture-MS Homo sapiens 30 CHST10   Affinity Capture-MS Homo sapiens 31 PDLIM5 10611 Affinity Capture-MS Homo sapiens 32 MYC   Affinity Capture-MS Homo sapiens 33 BAG1 573 Affinity Capture-MS Homo sapiens 34 IGHG1 3500 Affinity Capture-MS Homo sapiens 35 SARAF   Affinity Capture-MS Homo sapiens 36 MCM2 4171 Affinity Capture-MS Homo sapiens 37 PIGT 51604 Affinity Capture-MS Homo sapiens 38 PINK1   Affinity Capture-MS Homo sapiens 39 GOT1 2805 Affinity Capture-MS Homo sapiens 40 IGHV4-31 28396 Affinity Capture-MS Homo sapiens 41 ARMC10 83787 Affinity Capture-MS Homo sapiens 42 P2RX6   Affinity Capture-MS Homo sapiens 43 UBE2U   Affinity Capture-MS Homo sapiens 44 UPP1 7378 Affinity Capture-MS Homo sapiens 45 PHF11 51131 Affinity Capture-MS Homo sapiens View the network

Pathways in which IGHG4 is involved

No pathways found