Gene description for KCMF1
Gene name potassium channel modulatory factor 1
Gene symbol KCMF1
Other names/aliases DEBT91
FIGC
PCMF
ZZZ1
Species Homo sapiens
 Database cross references - KCMF1
ExoCarta ExoCarta_56888
Vesiclepedia VP_56888
Entrez Gene 56888
HGNC 20589
MIM 614719
UniProt Q9P0J7  
 KCMF1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for KCMF1
Molecular Function
    zinc ion binding GO:0008270 IEA
    ubiquitin protein ligase activity GO:0061630 IDA
Biological Process
    ubiquitin-dependent protein catabolic process GO:0006511 IDA
    response to oxidative stress GO:0006979 IDA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IDA
    protein K63-linked ubiquitination GO:0070534 IDA
    protein K48-linked ubiquitination GO:0070936 IDA
    synaptic signaling GO:0099536 IBA
    negative regulation of HRI-mediated signaling GO:0141191 IDA
Subcellular Localization
    extracellular region GO:0005576 TAS
    cytoplasm GO:0005737 IDA
    lysosome GO:0005764 IEA
    late endosome GO:0005770 IEA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IBA
    synapse GO:0045202 IEA
    ficolin-1-rich granule lumen GO:1904813 TAS
 Experiment description of studies that identified KCMF1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for KCMF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 A1BG 1
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 UBE2A  
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
4 QARS 5859
Affinity Capture-MS Homo sapiens
5 FSTL4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 NUP188 23511
Affinity Capture-MS Homo sapiens
7 ACOT9 23597
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 PSMD11 5717
Co-fractionation Homo sapiens
9 PTX3 5806
Affinity Capture-MS Homo sapiens
10 CFC1  
Affinity Capture-MS Homo sapiens
11 EMID1  
Affinity Capture-MS Homo sapiens
12 BYSL 705
Co-fractionation Homo sapiens
13 UBC 7316
Affinity Capture-MS Homo sapiens
14 VWA5A 4013
Affinity Capture-MS Homo sapiens
15 WDR26 80232
Co-fractionation Homo sapiens
16 STX11 8676
Affinity Capture-MS Homo sapiens
17 EMILIN1 11117
Affinity Capture-MS Homo sapiens
18 TRMU  
Affinity Capture-MS Homo sapiens
19 TGFB1 7040
Affinity Capture-MS Homo sapiens
20 SARS2 54938
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 CPSF7 79869
Affinity Capture-MS Homo sapiens
22 PSMD12 5718
Co-fractionation Homo sapiens
23 POU5F1  
Affinity Capture-MS Homo sapiens
24 NUP93 9688
Affinity Capture-MS Homo sapiens
25 ELMO3 79767
Affinity Capture-MS Homo sapiens
26 MRS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 TESPA1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 UCHL5 51377
Co-fractionation Homo sapiens
29 FBXW5  
Affinity Capture-MS Homo sapiens
30 RGS4  
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
31 KLF16  
Affinity Capture-MS Homo sapiens
32 UBR4 23352
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
33 C2orf27B  
Affinity Capture-MS Homo sapiens
34 MCC 4163
Affinity Capture-MS Homo sapiens
35 CHIA  
Affinity Capture-MS Homo sapiens
36 PSMC1 5700
Co-fractionation Homo sapiens
37 GOLGA2 2801
Affinity Capture-MS Homo sapiens
38 PNO1 56902
Co-fractionation Homo sapiens
39 TBC1D32 221322
Proximity Label-MS Homo sapiens
40 ACACA 31
Positive Genetic Homo sapiens
41 MIOX  
Affinity Capture-MS Homo sapiens
42 RASSF9  
Affinity Capture-MS Homo sapiens
43 VCP 7415
Affinity Capture-MS Homo sapiens
44 ARRDC2  
Affinity Capture-MS Homo sapiens
45 OGT 8473
Affinity Capture-MS Homo sapiens
46 FIGF 2277
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 C1orf115  
Affinity Capture-MS Homo sapiens
48 NIPSNAP1 8508
Affinity Capture-MS Homo sapiens
49 FAM73B  
Affinity Capture-MS Homo sapiens
50 UPP1 7378
Affinity Capture-MS Homo sapiens
51 CST9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 LYPD6B 130576
Affinity Capture-MS Homo sapiens
53 GPIHBP1  
Affinity Capture-MS Homo sapiens
54 OR52B2  
Affinity Capture-MS Homo sapiens
55 PPM1M  
Affinity Capture-MS Homo sapiens
56 XPO1 7514
Affinity Capture-MS Homo sapiens
57 IL25  
Affinity Capture-MS Homo sapiens
58 PSMD8 5714
Co-fractionation Homo sapiens
59 KCMF1 56888
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
60 PRCP 5547
Affinity Capture-MS Homo sapiens
61 CUL3 8452
Affinity Capture-MS Homo sapiens
62 EGFL7 51162
Affinity Capture-MS Homo sapiens
63 TFPT  
Affinity Capture-MS Homo sapiens
64 SERINC5 256987
Affinity Capture-MS Homo sapiens
65 PSMD7 5713
Co-fractionation Homo sapiens
66 ELP2 55250
Affinity Capture-MS Homo sapiens
67 UBE2D2 7322
Reconstituted Complex Homo sapiens
68 TJP2 9414
Co-fractionation Homo sapiens
69 RPS13 6207
Co-fractionation Homo sapiens
70 RASSF10  
Affinity Capture-MS Homo sapiens
71 ALDH3B2  
Affinity Capture-MS Homo sapiens
72 TXNL1 9352
Co-fractionation Homo sapiens
73 PSMC5 5705
Co-fractionation Homo sapiens
74 NUP214 8021
Affinity Capture-MS Homo sapiens
75 HDLBP 3069
Co-fractionation Homo sapiens
76 CEP85  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 CD96  
Affinity Capture-MS Homo sapiens
78 POGLUT1 56983
Affinity Capture-MS Homo sapiens
79 FOXK1 221937
Affinity Capture-MS Homo sapiens
80 WIBG 84305
Co-fractionation Homo sapiens
81 RAPSN  
Affinity Capture-MS Homo sapiens
82 ZRANB1 54764
Affinity Capture-MS Homo sapiens
83 HERC1 8925
Co-fractionation Homo sapiens
84 NIPSNAP3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 GRK5 2869
Affinity Capture-MS Homo sapiens
86 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
87 FASN 2194
Negative Genetic Homo sapiens
88 TFDP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 PARP1 142
Proximity Label-MS Homo sapiens
90 WDR77 79084
Affinity Capture-MS Homo sapiens
91 C15orf27  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 MDK 4192
Affinity Capture-MS Homo sapiens
93 ABHD10 55347
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 CLEC2D  
Affinity Capture-MS Homo sapiens
95 IKBKG 8517
Co-fractionation Homo sapiens
96 HSPB1 3315
Two-hybrid Homo sapiens
97 ELFN2 114794
Affinity Capture-MS Homo sapiens
98 LY6G5C  
Affinity Capture-MS Homo sapiens
99 SSBP1 6742
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 IDH2 3418
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
101 EDA  
Affinity Capture-MS Homo sapiens
102 UBE2B 7320
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 FLNC 2318
Co-fractionation Homo sapiens
104 KRAS 3845
Negative Genetic Homo sapiens
105 CAPN10  
Affinity Capture-MS Homo sapiens
106 STK32C  
Affinity Capture-MS Homo sapiens
107 PSMD3 5709
Co-fractionation Homo sapiens
108 EIF3L 51386
Co-fractionation Homo sapiens
109 PSMD13 5719
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which KCMF1 is involved
PathwayEvidenceSource
Immune System TAS Reactome
Innate Immune System TAS Reactome
Neutrophil degranulation TAS Reactome





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