Gene description for UBE2B
Gene name ubiquitin-conjugating enzyme E2B
Gene symbol UBE2B
Other names/aliases E2-17kDa
Species Homo sapiens
 Database cross references - UBE2B
ExoCarta ExoCarta_7320
Entrez Gene 7320
HGNC 12473
MIM 179095
UniProt P63146  
 UBE2B identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for UBE2B
Molecular Function
    ubiquitin protein ligase activity GO:0061630 IBA
    ligase activity GO:0016874 IEA
    ubiquitin protein ligase binding GO:0031625 IPI
    ubiquitin-protein transferase activity GO:0004842 IMP
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
Biological Process
    maintenance of chromatin silencing GO:0006344 IEA
    cellular response to insulin stimulus GO:0032869 IEA
    sperm axoneme assembly GO:0007288 IEA
    negative regulation of apoptotic process GO:0043066 IEA
    response to UV GO:0009411 IGI
    protein stabilization GO:0050821 IMP
    ubiquitin-dependent protein catabolic process GO:0006511 NAS
    protein ubiquitination GO:0016567 IDA
    protein K63-linked ubiquitination GO:0070534 IDA
    response to drug GO:0042493 IDA
    spermatogenesis GO:0007283 TAS
    translesion synthesis GO:0019985 TAS
    positive regulation of reciprocal meiotic recombination GO:0010845 IEA
    response to hypoxia GO:0001666 IEA
    chiasma assembly GO:0051026 IEA
    protein polyubiquitination GO:0000209 IMP
    synaptonemal complex organization GO:0070193 IEA
    DNA repair GO:0006281 TAS
    protein K48-linked ubiquitination GO:0070936 IDA
    negative regulation of cAMP-mediated signaling GO:0043951 IDA
    in utero embryonic development GO:0001701 IEA
    protein monoubiquitination GO:0006513 IMP
    protein K11-linked ubiquitination GO:0070979 IDA
    negative regulation of histone phosphorylation GO:0033128 IEA
    cellular response to DNA damage stimulus GO:0006974 IDA
    postreplication repair GO:0006301 NAS
    histone lysine demethylation GO:0070076 IEA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IDA
    canonical Wnt signaling pathway GO:0060070 ISS
    protein autoubiquitination GO:0051865 IDA
    histone H2A ubiquitination GO:0033522 IMP
    DNA damage response, detection of DNA damage GO:0042769 TAS
Subcellular Localization
    HULC complex GO:0033503 IDA
    nucleoplasm GO:0005654 TAS
    nuclear chromatin GO:0000790 IBA
    plasma membrane GO:0005886 IEA
    replication fork GO:0005657 IDA
    cytoplasm GO:0005737 IDA
    XY body GO:0001741 IEA
    chromatin GO:0000785 ISS
    nucleus GO:0005634 IDA
 Experiment description of studies that identified UBE2B in exosomes
Experiment ID 235
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for UBE2B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBR2 23304
Affinity Capture-Western Homo sapiens
2 CRMP1 1400
Two-hybrid Homo sapiens
3 MED31  
Two-hybrid Homo sapiens
4 UNC119  
Two-hybrid Homo sapiens
5 UBR1  
Two-hybrid Homo sapiens
6 RAD18  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml

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