Gene description for UBE2C
Gene name ubiquitin-conjugating enzyme E2C
Gene symbol UBE2C
Other names/aliases UBCH10
dJ447F3.2
Species Homo sapiens
 Database cross references - UBE2C
ExoCarta ExoCarta_11065
Vesiclepedia VP_11065
Entrez Gene 11065
HGNC 15937
MIM 605574
UniProt O00762  
 UBE2C identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for UBE2C
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IDA
    ubiquitin-protein transferase activity GO:0004842 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ubiquitin-like protein ligase binding GO:0044389 IPI
    ubiquitin protein ligase activity GO:0061630 TAS
    ubiquitin conjugating enzyme activity GO:0061631 IBA
    ubiquitin conjugating enzyme activity GO:0061631 IDA
Biological Process
    protein polyubiquitination GO:0000209 IBA
    ubiquitin-dependent protein catabolic process GO:0006511 IDA
    exit from mitosis GO:0010458 IMP
    free ubiquitin chain polymerization GO:0010994 IDA
    protein ubiquitination GO:0016567 IDA
    regulation of mitotic metaphase/anaphase transition GO:0030071 IBA
    anaphase-promoting complex-dependent catabolic process GO:0031145 IBA
    anaphase-promoting complex-dependent catabolic process GO:0031145 IDA
    anaphase-promoting complex-dependent catabolic process GO:0031145 TAS
    positive regulation of exit from mitosis GO:0031536 IMP
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IDA
    positive regulation of mitotic metaphase/anaphase transition GO:0045842 IDA
    cell division GO:0051301 IEA
    protein K48-linked ubiquitination GO:0070936 IDA
    protein K11-linked ubiquitination GO:0070979 IDA
    positive regulation of ubiquitin protein ligase activity GO:1904668 TAS
Subcellular Localization
    ubiquitin ligase complex GO:0000151 IDA
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 TAS
    anaphase-promoting complex GO:0005680 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified UBE2C in exosomes
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for UBE2C
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BAG3 9531
Affinity Capture-MS Homo sapiens
2 ATP6V1B2 526
Co-fractionation Homo sapiens
3 RELA 5970
Biochemical Activity Homo sapiens
4 TRIP6 7205
Co-fractionation Homo sapiens
5 UBE2S  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
6 UBQLN4 56893
Co-fractionation Homo sapiens
7 KIAA0101  
Affinity Capture-Western Homo sapiens
8 RNF7  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
9 CDC20 991
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
10 POLM  
Biochemical Activity Homo sapiens
11 Ube2c  
Affinity Capture-MS Mus musculus
12 MAD2L1 4085
Co-fractionation Homo sapiens
13 ATP6V1F 9296
Co-fractionation Homo sapiens
14 ZRANB2 9406
Co-fractionation Homo sapiens
15 USP34 9736
Co-fractionation Homo sapiens
16 RNF167 26001
Reconstituted Complex Homo sapiens
17 AHCYL1 10768
Co-fractionation Homo sapiens
18 TYMS 7298
Co-fractionation Homo sapiens
19 UBE2V1 7335
Co-fractionation Homo sapiens
20 UNK  
Co-fractionation Homo sapiens
21 UBXN7 26043
Co-fractionation Homo sapiens
22 RNF31 55072
Reconstituted Complex Homo sapiens
23 AURKA 6790
Biochemical Activity Homo sapiens
24 PSMC2 5701
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
25 Anapc11  
Reconstituted Complex Mus musculus
26 UBA1 7317
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
27 UBC 7316
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
28 BUB1B  
Co-fractionation Homo sapiens
29 USP9X 8239
Co-fractionation Homo sapiens
30 CKAP2  
Biochemical Activity Homo sapiens
31 UBE2C 11065
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
32 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
Affinity Capture-RNA Homo sapiens
33 PSMD4 5710
Reconstituted Complex Homo sapiens
34 PCMT1 5110
Co-fractionation Homo sapiens
35 RNF216  
Affinity Capture-Western Homo sapiens
36 ZFYVE19 84936
Co-fractionation Homo sapiens
37 SEC24A 10802
Co-fractionation Homo sapiens
38 MAPK7  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
39 ANAPC2 29882
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
40 YAP1 10413
Co-fractionation Homo sapiens
41 TNNC1  
Two-hybrid Homo sapiens
42 GFER  
Co-fractionation Homo sapiens
43 UBASH3B 84959
Biochemical Activity Homo sapiens
44 RAG1  
Reconstituted Complex Homo sapiens
45 TRIM28 10155
Reconstituted Complex Homo sapiens
46 CDH1 999
Co-crystal Structure Homo sapiens
47 UBE4B 10277
Co-fractionation Homo sapiens
48 PSMA5 5686
Co-fractionation Homo sapiens
49 UBE2B 7320
Co-fractionation Homo sapiens
50 TRIM21 6737
Reconstituted Complex Homo sapiens
51 SAR1B 51128
Affinity Capture-MS Homo sapiens
52 TRIM13  
Reconstituted Complex Homo sapiens
53 SSSCA1 10534
Co-fractionation Homo sapiens
54 PSMB5 5693
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
55 FZR1  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
56 CARKD 55739
Co-fractionation Homo sapiens
57 ENO2 2026
Two-hybrid Homo sapiens
58 TTC9C 283237
Co-fractionation Homo sapiens
59 PVR 5817
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 TAGLN2 8407
Co-fractionation Homo sapiens
61 Rag1  
Reconstituted Complex Mus musculus
62 UBE2D3 7323
Co-fractionation Homo sapiens
63 PSMC6 5706
Co-fractionation Homo sapiens
64 TRIM29 23650
Reconstituted Complex Homo sapiens
65 DTX1  
Two-hybrid Homo sapiens
66 RNF6  
Reconstituted Complex Homo sapiens
67 UBQLN1 29979
Co-fractionation Homo sapiens
68 ITCH 83737
Reconstituted Complex Homo sapiens
69 ANAPC11  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
70 PPM1G 5496
Co-fractionation Homo sapiens
71 CXCR1  
Two-hybrid Homo sapiens
72 UBXN1 51035
Co-fractionation Homo sapiens
73 KLHL2 11275
Two-hybrid Homo sapiens
74 UBE3D  
Reconstituted Complex Homo sapiens
75 TTLL12 23170
Co-fractionation Homo sapiens
76 PCGF3  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which UBE2C is involved
PathwayEvidenceSource
Aberrant regulation of mitotic cell cycle due to RB1 defects TAS Reactome
Aberrant regulation of mitotic exit in cancer due to RB1 defects TAS Reactome
Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins TAS Reactome
Adaptive Immune System TAS Reactome
Antigen processing: Ubiquitination & Proteasome degradation TAS Reactome
APC-Cdc20 mediated degradation of Nek2A TAS Reactome
APC/C-mediated degradation of cell cycle proteins TAS Reactome
APC/C:Cdc20 mediated degradation of Cyclin B TAS Reactome
APC/C:Cdc20 mediated degradation of mitotic proteins TAS Reactome
APC/C:Cdc20 mediated degradation of Securin TAS Reactome
APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 TAS Reactome
APC:Cdc20 mediated degradation of cell cycle proteins prior to satisfation of the cell cycle checkpoint TAS Reactome
Assembly of the pre-replicative complex TAS Reactome
Autodegradation of Cdh1 by Cdh1:APC/C TAS Reactome
Cdc20:Phospho-APC/C mediated degradation of Cyclin A TAS Reactome
CDK-mediated phosphorylation and removal of Cdc6 TAS Reactome
Cell Cycle TAS Reactome
Cell Cycle Checkpoints TAS Reactome
Cell Cycle, Mitotic TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Cellular Senescence TAS Reactome
Class I MHC mediated antigen processing & presentation TAS Reactome
Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase TAS Reactome
Disease TAS Reactome
Diseases of mitotic cell cycle TAS Reactome
DNA Replication TAS Reactome
DNA Replication Pre-Initiation TAS Reactome
Gene expression (Transcription) TAS Reactome
Generic Transcription Pathway TAS Reactome
Immune System TAS Reactome
Inactivation of APC/C via direct inhibition of the APC/C complex TAS Reactome
Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components TAS Reactome
M Phase TAS Reactome
Metabolism of proteins TAS Reactome
Mitotic Anaphase TAS Reactome
Mitotic Metaphase and Anaphase TAS Reactome
Mitotic Spindle Checkpoint TAS Reactome
Phosphorylation of the APC/C TAS Reactome
Post-translational protein modification TAS Reactome
Protein ubiquitination TAS Reactome
Regulation of APC/C activators between G1/S and early anaphase TAS Reactome
Regulation of mitotic cell cycle TAS Reactome
RNA Polymerase II Transcription TAS Reactome
S Phase TAS Reactome
Senescence-Associated Secretory Phenotype (SASP) TAS Reactome
Separation of Sister Chromatids TAS Reactome
Switching of origins to a post-replicative state TAS Reactome
Synthesis of active ubiquitin: roles of E1 and E2 enzymes TAS Reactome
Synthesis of DNA TAS Reactome
Transcriptional Regulation by VENTX TAS Reactome





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