Experiment description of studies that identified UBASH3B in exosomes

1 Experiment ID 226 MISEV standards ✔ EM Biophysical techniques ✔ GAPDH|CD9|FLOT1 Enriched markers ✔ LMNA|H2AFX|ATP5A1|TOMM20 Negative markers ✘ Particle analysis Identified molecule mRNA Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa) PubMed ID 26027894     Organism Homo sapiens Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting" Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar" Journal name Journal of Extracellular Vesicles Publication year 2015 Sample Endothelial cells Sample name HMEC-1 Isolation/purification methods Differential ultracentrifugation Sucrose density gradient Flotation density 1.10 g/mL Molecules identified in the study miRNA Protein snoRNA lncRNA yRNA snRNA mRNA ncRNA mtRNA vtRNA scaRNA lincRNA Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa) Western blotting

2 Experiment ID 237 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocytes Sample name MIHA Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RNA Sequencing

3 Experiment ID 488 MISEV standards ✔ EM Biophysical techniques ✔ CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 36408942     Organism Homo sapiens Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ." Journal name Proteomics Publication year 2023 Sample Mesenchymal stem cells Sample name UCMSC Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

4 Experiment ID 231 MISEV standards ✘ Biophysical techniques ✔ Alix|CD63|CD9 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 1 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Lipids Methods used in the study Western blotting Mass spectrometry

5 Experiment ID 232 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 2 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

6 Experiment ID 233 MISEV standards ✘ Biophysical techniques ✘ Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25332113     Organism Homo sapiens Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G." Journal name Transfusion Publication year 2015 Sample Platelets Sample name PL-Exs - Rep 3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient Flotation density 1.12-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

7 Experiment ID 217 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|CD81|CD9|CD63 Enriched markers ✘ Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23844026     Organism Homo sapiens Experiment description Characterization of human thymic exosomes. Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O." Journal name PLoS One Publication year 2013 Sample Thymus Sample name Normal-Thymus Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry

Protein-protein interactions for UBASH3B

Protein Interactor ExoCarta ID Identification method PubMed Species 1 UBE2H 7328 Biochemical Activity Homo sapiens 2 HNRNPH1 3187 Affinity Capture-MS Homo sapiens 3 KRT4 3851 Affinity Capture-MS Homo sapiens 4 KRT73 319101 Affinity Capture-MS Homo sapiens 5 PRR35   Two-hybrid Homo sapiens 6 CCT3 7203 Affinity Capture-MS Homo sapiens 7 KRT6B 3854 Affinity Capture-MS Homo sapiens 8 KRT1 3848 Affinity Capture-MS Homo sapiens 9 SHC1 6464 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 10 CSK 1445 Affinity Capture-MS Homo sapiens 11 KRT6A 3853 Affinity Capture-MS Homo sapiens 12 KRT17 3872 Affinity Capture-MS Homo sapiens 13 CRK 1398 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 14 ABL1 25 Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens 15 GFAP 2670 Affinity Capture-MS Homo sapiens 16 HSPA1L 3305 Affinity Capture-MS Homo sapiens 17 KIF20A 10112 Affinity Capture-Western Homo sapiens Co-localization Homo sapiens 18 HSP90AB1 3326 Affinity Capture-MS Homo sapiens 19 FAM168A   Two-hybrid Homo sapiens Two-hybrid Homo sapiens 20 CCT6B 10693 Affinity Capture-MS Homo sapiens 21 SAP30 8819 Affinity Capture-MS Homo sapiens 22 HDAC1 3065 Affinity Capture-MS Homo sapiens 23 LGALS3BP 3959 Affinity Capture-MS Homo sapiens 24 LMO2   Two-hybrid Homo sapiens 25 DTX3   Two-hybrid Homo sapiens 26 DMRT3   Two-hybrid Homo sapiens 27 IQGAP1 8826 Affinity Capture-MS Homo sapiens 28 KHDRBS1 10657 Protein-peptide Homo sapiens 29 SYK 6850 Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Biochemical Activity Homo sapiens Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens 30 EWSR1 2130 Affinity Capture-MS Homo sapiens 31 KRT5 3852 Affinity Capture-MS Homo sapiens 32 HSPA7 3311 Affinity Capture-MS Homo sapiens 33 KPRP 448834 Two-hybrid Homo sapiens 34 WASL 8976 Two-hybrid Homo sapiens 35 DDX6 1656 Affinity Capture-MS Homo sapiens 36 CBLB 868 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 37 NCK1 4690 Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 38 DLGAP2   Two-hybrid Homo sapiens 39 HSPA8 3312 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 40 TMEM255A   Affinity Capture-MS Homo sapiens 41 SLC25A48   Two-hybrid Homo sapiens 42 KRT8 3856 Affinity Capture-MS Homo sapiens 43 RNF41   Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens 44 SPTA1   Affinity Capture-MS Homo sapiens 45 PARK2   Reconstituted Complex Homo sapiens 46 CCDC33 80125 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 47 CBL 867 Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens 48 DAZAP2   Two-hybrid Homo sapiens Two-hybrid Homo sapiens 49 ACACA 31 Affinity Capture-MS Homo sapiens 50 UBASH3B 84959 Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens 51 ESRRB   Affinity Capture-MS Homo sapiens 52 TUBA1B 10376 Affinity Capture-MS Homo sapiens 53 C19orf26 255057 Affinity Capture-MS Homo sapiens 54 MYH9 4627 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 55 ACTN4 81 Affinity Capture-MS Homo sapiens 56 REL 5966 Two-hybrid Homo sapiens 57 KRT9 3857 Affinity Capture-MS Homo sapiens 58 HIST1H1E 3008 Affinity Capture-MS Homo sapiens 59 S100A8 6279 Affinity Capture-MS Homo sapiens 60 DNAJA1 3301 Affinity Capture-MS Homo sapiens 61 KRT14 3861 Affinity Capture-MS Homo sapiens 62 HIST1H1C 3006 Affinity Capture-MS Homo sapiens 63 ACTB 60 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 64 MYH10 4628 Affinity Capture-MS Homo sapiens 65 KRT2 3849 Affinity Capture-MS Homo sapiens 66 UBE2D3 7323 Biochemical Activity Homo sapiens 67 CCDC74B   Two-hybrid Homo sapiens 68 KRT79 338785 Affinity Capture-MS Homo sapiens 69 TCP1 6950 Affinity Capture-MS Homo sapiens 70 VPS37B 79720 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 71 BBC3   Two-hybrid Homo sapiens 72 TXK   Affinity Capture-MS Homo sapiens 73 DISC1 27185 Two-hybrid Homo sapiens 74 FAM189B   Affinity Capture-MS Homo sapiens 75 HSPA5 3309 Affinity Capture-MS Homo sapiens 76 TUBB 203068 Affinity Capture-MS Homo sapiens 77 MCCC2 64087 Affinity Capture-MS Homo sapiens 78 TNKS 8658 Two-hybrid Homo sapiens 79 CCT6A 908 Affinity Capture-MS Homo sapiens 80 HOXB2   Two-hybrid Homo sapiens 81 UBC 7316 Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-MS Homo sapiens 82 CUL3 8452 Reconstituted Complex Homo sapiens 83 EFS   Two-hybrid Homo sapiens 84 UBE2E1   Biochemical Activity Homo sapiens 85 PRR27 401137 Affinity Capture-MS Homo sapiens 86 CORO1C 23603 Affinity Capture-MS Homo sapiens 87 SPTBN1 6711 Affinity Capture-MS Homo sapiens 88 MAP3K1 4214 Biochemical Activity Homo sapiens 89 AURKB 9212 Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens Co-localization Homo sapiens 90 FAM209A 200232 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 91 UBE2D2 7322 Biochemical Activity Homo sapiens 92 MYO1D 4642 Affinity Capture-MS Homo sapiens 93 UBASH3A 53347 Affinity Capture-MS Homo sapiens 94 PKP4 8502 Two-hybrid Homo sapiens 95 EGFR 1956 Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens 96 HNRNPH2 3188 Affinity Capture-MS Homo sapiens 97 KAT5   Two-hybrid Homo sapiens 98 HIST1H1A 3024 Affinity Capture-MS Homo sapiens 99 INPP5D 3635 Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 100 HIST1H1T 3010 Affinity Capture-MS Homo sapiens 101 ENTHD2   Two-hybrid Homo sapiens 102 KRT16 3868 Affinity Capture-MS Homo sapiens 103 DOC2A   Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens 104 ARAP1 116985 Affinity Capture-MS Homo sapiens 105 UBE2D1 7321 Biochemical Activity Homo sapiens 106 RPS27A 6233 Affinity Capture-MS Homo sapiens 107 TP53BP2   Two-hybrid Homo sapiens 108 SPTAN1 6709 Affinity Capture-MS Homo sapiens 109 CCT8 10694 Affinity Capture-MS Homo sapiens 110 SPRY2 10253 Two-hybrid Homo sapiens 111 GRB2 2885 Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens 112 INPPL1 3636 Affinity Capture-MS Homo sapiens 113 DSC1 1823 Affinity Capture-MS Homo sapiens 114 KRT10 3858 Affinity Capture-MS Homo sapiens 115 INCA1   Two-hybrid Homo sapiens 116 HNRNPA2B1 3181 Affinity Capture-MS Homo sapiens 117 LZTS2 84445 Two-hybrid Homo sapiens 118 UBE2C 11065 Biochemical Activity Homo sapiens 119 ELAVL1 1994 Affinity Capture-RNA Homo sapiens 120 RCHY1   Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens 121 CHD4 1108 Affinity Capture-MS Homo sapiens 122 HSP90AA1 3320 Affinity Capture-MS Homo sapiens 123 CCT7 10574 Affinity Capture-MS Homo sapiens 124 DCD 117159 Affinity Capture-MS Homo sapiens 125 ACTG1 71 Affinity Capture-MS Homo sapiens 126 TCF4   Two-hybrid Homo sapiens 127 EEF1A1P5 158078 Affinity Capture-MS Homo sapiens 128 ERRFI1 54206 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 129 HNRNPF 3185 Affinity Capture-MS Homo sapiens 130 HNRNPU 3192 Affinity Capture-MS Homo sapiens 131 BCR 613 Affinity Capture-MS Homo sapiens 132 HIST1H1D 3007 Affinity Capture-MS Homo sapiens 133 HSPA1B 3304 Affinity Capture-MS Homo sapiens 134 MSRA 4482 Affinity Capture-MS Homo sapiens 135 NPTX2   Affinity Capture-MS Homo sapiens View the network

Pathways in which UBASH3B is involved

No pathways found