Gene description for TNKS
Gene name tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase
Gene symbol TNKS
Other names/aliases ARTD5
PARP-5a
PARP5A
PARPL
TIN1
TINF1
TNKS1
pART5
Species Homo sapiens
 Database cross references - TNKS
ExoCarta ExoCarta_8658
Entrez Gene 8658
HGNC 11941
MIM 603303
UniProt O95271  
 TNKS identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for TNKS
Molecular Function
    NAD+ ADP-ribosyltransferase activity GO:0003950 TAS
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IDA
Biological Process
    protein polyubiquitination GO:0000209 IDA
    mitotic spindle organization GO:0007052 TAS
    mitotic nuclear division GO:0007067 IEA
    positive regulation of telomere maintenance via telomerase GO:0032212 IMP
    negative regulation of DNA binding GO:0043392 IDA
    spindle assembly GO:0051225 TAS
    Wnt signaling pathway GO:0016055 IEA
    protein localization to chromosome, telomeric region GO:0070198 IMP
    peptidyl-threonine phosphorylation GO:0018107 IDA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
    mRNA transport GO:0051028 IEA
    peptidyl-serine phosphorylation GO:0018105 IDA
    protein ADP-ribosylation GO:0006471 IDA
    cell division GO:0051301 IEA
    regulation of telomere maintenance via telomerase GO:0032210 IC
    protein poly-ADP-ribosylation GO:0070212 IDA
    protein auto-ADP-ribosylation GO:0070213 IDA
    protein transport GO:0015031 IEA
    positive regulation of canonical Wnt signaling pathway GO:0090263 TAS
Subcellular Localization
    chromosome, telomeric region GO:0000781 IDA
    nuclear membrane GO:0031965 TAS
    nuclear pore GO:0005643 TAS
    spindle pole GO:0000922 IEA
    cytosol GO:0005829 TAS
    pericentriolar material GO:0000242 TAS
    chromosome, centromeric region GO:0000775 IEA
    Golgi apparatus GO:0005794 IDA
    nuclear chromosome, telomeric region GO:0000784 IDA
    Golgi membrane GO:0000139 IEA
 Experiment description of studies that identified TNKS in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for TNKS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TNKS1BP1 85456
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
2 VPS33A 65082
Invivo Homo sapiens
3 MCL1 4170
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
4 FNBP1 23048
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
5 LNPEP 4012
Invivo Homo sapiens
Invitro Homo sapiens
6 TERF1 7013
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
7 NUMA1 4926
Reconstituted Complex Homo sapiens
8 POT1  
Affinity Capture-Western Homo sapiens
9 TNKS2 80351
Invivo Homo sapiens
10 IL1RN 3557
Invivo Homo sapiens
Invitro Homo sapiens
View the network image/svg+xml



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