Gene description for Cct2
Gene name chaperonin containing Tcp1, subunit 2 (beta)
Gene symbol Cct2
Other names/aliases Cctb
Species Mus musculus
 Database cross references - Cct2
ExoCarta ExoCarta_12461
Vesiclepedia VP_12461
Entrez Gene 12461
UniProt P80314  
 Cct2 identified in sEVs derived from the following tissue/cell type
Brain cancer cells 19109410    
Colon cancer cells 37309723    
Fibroblasts 23260141    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cct2
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    protein folding chaperone GO:0044183 ISO
    unfolded protein binding GO:0051082 IBA
    unfolded protein binding GO:0051082 IEA
    ATP-dependent protein folding chaperone GO:0140662 IEA
Biological Process
    protein folding GO:0006457 IBA
    protein folding GO:0006457 ISO
    binding of sperm to zona pellucida GO:0007339 IDA
    positive regulation of telomere maintenance via telomerase GO:0032212 IEA
    positive regulation of telomere maintenance via telomerase GO:0032212 ISO
    protein stabilization GO:0050821 IEA
    protein stabilization GO:0050821 ISO
    chaperone mediated protein folding independent of cofactor GO:0051086 IEA
    chaperone mediated protein folding independent of cofactor GO:0051086 ISO
    chaperone-mediated protein complex assembly GO:0051131 ISO
    chaperone-mediated protein folding GO:0061077 ISO
    scaRNA localization to Cajal body GO:0090666 IEA
    scaRNA localization to Cajal body GO:0090666 ISO
    positive regulation of establishment of protein localization to telomere GO:1904851 ISO
    positive regulation of telomerase RNA localization to Cajal body GO:1904874 IEA
    positive regulation of telomerase RNA localization to Cajal body GO:1904874 ISO
Subcellular Localization
    zona pellucida receptor complex GO:0002199 IDA
    cytosol GO:0005829 ISO
    chaperonin-containing T-complex GO:0005832 IBA
    chaperonin-containing T-complex GO:0005832 IDA
    chaperonin-containing T-complex GO:0005832 ISO
    microtubule GO:0005874 IEA
    microtubule GO:0005874 ISO
    myelin sheath GO:0043209 HDA
    cell body GO:0044297 IDA
 Experiment description of studies that identified Cct2 in sEVs
1
Experiment ID 26
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|GAPDH|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19109410    
Organism Mus musculus
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors "Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD"
Journal name FASEB
Publication year 2008
Sample Brain cancer cells
Sample name SMA560vIII
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
2
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
8
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cct2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IGBP1 3476
Affinity Capture-MS Homo sapiens
2 MLST8 64223
Affinity Capture-MS Homo sapiens
3 Elf5  
Affinity Capture-MS Mus musculus
4 Cct6a 12466
Co-fractionation Mus musculus
5 TUBB 203068
Affinity Capture-MS Homo sapiens
6 CDC20 991
Affinity Capture-MS Homo sapiens
7 Strn3  
Co-fractionation Mus musculus
8 CCT3 7203
Affinity Capture-MS Homo sapiens
9 PDCL3 79031
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 CSTF1 1477
Affinity Capture-MS Homo sapiens
11 KIF21A 55605
Affinity Capture-MS Homo sapiens
12 Pou5f1  
Affinity Capture-MS Mus musculus
13 Cct3 12462
Co-fractionation Mus musculus
14 TNKS 8658
Affinity Capture-MS Homo sapiens
15 Kcnma1  
Affinity Capture-MS Mus musculus
16 PDCL 5082
Affinity Capture-MS Homo sapiens
17 CCT6A 908
Affinity Capture-MS Homo sapiens
18 WDR92 116143
Affinity Capture-MS Homo sapiens
19 Atxn1  
Proximity Label-MS Mus musculus
20 TCP1 6950
Affinity Capture-MS Homo sapiens
21 Kctd13  
Affinity Capture-MS Mus musculus
22 RPTOR 57521
Affinity Capture-MS Homo sapiens
23 EIF3I 8668
Affinity Capture-MS Homo sapiens
24 Tfe3  
Affinity Capture-MS Mus musculus
25 MTNR1A  
Co-purification Homo sapiens
26 CCT4 10575
Affinity Capture-MS Homo sapiens
27 HGH1 51236
Affinity Capture-MS Homo sapiens
28 PDPK1 5170
Affinity Capture-MS Homo sapiens
29 CCT7 10574
Affinity Capture-MS Homo sapiens
30 TXNDC9 10190
Affinity Capture-MS Homo sapiens
31 CCT5 22948
Affinity Capture-MS Homo sapiens
32 Tmem173  
Proximity Label-MS Mus musculus
33 ACTR2 10097
Affinity Capture-MS Homo sapiens
34 PDCD5 9141
Affinity Capture-MS Homo sapiens
35 Atg5  
Affinity Capture-RNA Mus musculus
36 GNB2 2783
Affinity Capture-MS Homo sapiens
37 ACTL8  
Affinity Capture-MS Homo sapiens
38 OSBPL9 114883
Affinity Capture-MS Homo sapiens
39 DCAF7 10238
Affinity Capture-MS Homo sapiens
40 CCT8 10694
Affinity Capture-MS Homo sapiens
41 SSSCA1 10534
Affinity Capture-MS Homo sapiens
42 Lnx1  
Affinity Capture-MS Mus musculus
43 DNAJC19 131118
Affinity Capture-MS Homo sapiens
44 ACTR1A 10121
Affinity Capture-MS Homo sapiens
45 BUB3 9184
Affinity Capture-MS Homo sapiens
46 Eed  
Affinity Capture-MS Mus musculus
47 Psmd2 21762
Co-fractionation Mus musculus
48 Mapt  
Affinity Capture-MS Mus musculus
49 Set 56086
Affinity Capture-MS Mus musculus
Reconstituted Complex Mus musculus
Affinity Capture-Western Mus musculus
50 ERI3 79033
Affinity Capture-MS Homo sapiens
51 RAE1 8480
Affinity Capture-MS Homo sapiens
52 Fancd2  
Affinity Capture-MS Mus musculus
53 ESCO2  
Affinity Capture-MS Homo sapiens
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