Gene description for CDC20
Gene name cell division cycle 20
Gene symbol CDC20
Other names/aliases CDC20A
bA276H19.3
p55CDC
Species Homo sapiens
 Database cross references - CDC20
ExoCarta ExoCarta_991
Vesiclepedia VP_991
Entrez Gene 991
HGNC 1723
MIM 603618
UniProt Q12834  
 CDC20 identified in exosomes derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for CDC20
Molecular Function
    enzyme binding GO:0019899 IPI
    protein C-terminus binding GO:0008022 IPI
    protein binding GO:0005515 IPI
Biological Process
    cell cycle GO:0007049 TAS
    mitotic spindle assembly checkpoint GO:0007094 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
    regulation of meiotic nuclear division GO:0040020 IEA
    mitotic cell cycle GO:0000278 TAS
    protein ubiquitination GO:0016567 IEA
    anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031145 TAS
    negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051436 TAS
    mitotic nuclear division GO:0007067 IEA
    positive regulation of cell proliferation GO:0008284 IEA
    positive regulation of synaptic plasticity GO:0031915 ISS
    positive regulation of synapse maturation GO:0090129 ISS
    regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051439 TAS
    activation of anaphase-promoting complex activity GO:0051488 IDA
    regulation of dendrite development GO:0050773 IEA
    positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition GO:0051437 TAS
    cell division GO:0051301 IEA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    anaphase-promoting complex GO:0005680 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
    spindle pole GO:0000922 IEA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    centrosome GO:0005813 IEA
    spindle GO:0005819 TAS
 Experiment description of studies that identified CDC20 in exosomes
1
Experiment ID 363
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ EV Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
EV Enriched markers
✔
DCLK1
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M.
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CDC20
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ANAPC7 51434
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
2 KRTHA3A  
Affinity Capture-MS Homo sapiens
3 KRTAP19-5  
Affinity Capture-MS Homo sapiens
4 HDAC2 3066
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
5 MAD2L1 4085
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
6 CGI-04  
Affinity Capture-MS Homo sapiens
7 AURKA 6790
Affinity Capture-Western Homo sapiens
8 ANAPC5 51433
Reconstituted Complex Homo sapiens
9 APC  
Invivo Homo sapiens
Invitro Homo sapiens
10 KRTAP9-3  
Affinity Capture-MS Homo sapiens
11 BUB1B  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
12 PTTG1  
Invivo Homo sapiens
Invitro Homo sapiens
13 HDAC1 3065
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
14 CDC16 8881
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
15 CCNA2 890
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
16 SPATC1  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
17 KRTAP9-2  
Affinity Capture-MS Homo sapiens
18 HSPC128  
Affinity Capture-MS Homo sapiens
19 MAD2L2 10459
Reconstituted Complex Homo sapiens
20 KRTHA2  
Affinity Capture-MS Homo sapiens
21 KRT31 3881
Affinity Capture-MS Homo sapiens
22 CDC27 996
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
23 KRTHB5  
Affinity Capture-MS Homo sapiens
24 FBXO5  
Invivo Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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