Gene description for MAD2L1
Gene name MAD2 mitotic arrest deficient-like 1 (yeast)
Gene symbol MAD2L1
Other names/aliases HSMAD2
MAD2
Species Homo sapiens
 Database cross references - MAD2L1
ExoCarta ExoCarta_4085
Vesiclepedia VP_4085
Entrez Gene 4085
HGNC 6763
MIM 601467
UniProt Q13257  
 MAD2L1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for MAD2L1
Molecular Function
    identical protein binding GO:0042802 IPI
    protein binding GO:0005515 IPI
    protein homodimerization activity GO:0042803 IPI
Biological Process
    negative regulation of mitotic cell cycle GO:0045930 IMP
    small GTPase mediated signal transduction GO:0007264 TAS
    negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051436 TAS
    negative regulation of APC-Cdc20 complex activity GO:0060564 IDA
    anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031145 TAS
    mitotic cell cycle checkpoint GO:0007093 IGI
    cell division GO:0051301 IEA
    negative regulation of apoptotic process GO:0043066 IMP
    mitotic cell cycle GO:0000278 TAS
    regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051439 TAS
    positive regulation of mitotic cell cycle spindle assembly checkpoint GO:0090267 IMP
    negative regulation of protein catabolic process GO:0042177 IDA
    mitotic spindle assembly checkpoint GO:0007094 TAS
    mitotic sister chromatid segregation GO:0000070 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    spindle pole GO:0000922 IEA
    cytosol GO:0005829 TAS
    condensed chromosome kinetochore GO:0000777 IEA
    perinuclear region of cytoplasm GO:0048471 IDA
    kinetochore GO:0000776 IDA
    nuclear pore GO:0005643 IDA
    mitotic spindle GO:0072686 IDA
 Experiment description of studies that identified MAD2L1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MAD2L1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAD1L1  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
2 NDC80 10403
Affinity Capture-MS Homo sapiens
3 DSTYK 25778
Affinity Capture-MS Homo sapiens
4 UBD  
Invitro Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
5 APC  
Invitro Homo sapiens
Invivo Homo sapiens
6 CSF2RB  
Invivo Homo sapiens
Invitro Homo sapiens
7 CDC16 8881
Invitro Homo sapiens
Invivo Homo sapiens
8 ADAM17 6868
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
9 ESR2  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
10 CDC20 991
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
11 MAD2L1BP  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
12 MAD2L1 4085
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 TSC22D4 81628
Two-hybrid Homo sapiens
14 BUB1B  
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
15 REV3L  
Invitro Homo sapiens
Two-hybrid Homo sapiens
16 CDC27 996
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here