Gene description for STK25
Gene name serine/threonine kinase 25
Gene symbol STK25
Other names/aliases SOK1
YSK1
Species Homo sapiens
 Database cross references - STK25
ExoCarta ExoCarta_10494
Entrez Gene 10494
HGNC 11404
MIM 602255
UniProt O00506  
 STK25 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for STK25
Molecular Function
    ATP binding GO:0005524 IEA
    metal ion binding GO:0046872 IEA
    protein binding GO:0005515 IPI
    protein kinase activity GO:0004672 TAS
    protein homodimerization activity GO:0042803 IDA
    protein serine/threonine kinase activity GO:0004674 IBA
Biological Process
    protein phosphorylation GO:0006468 IDA
    positive regulation of axonogenesis GO:0050772 IEA
    positive regulation of stress-activated MAPK cascade GO:0032874 IDA
    stress-activated protein kinase signaling cascade GO:0031098 IBA
    activation of protein kinase activity GO:0032147 IBA
    protein autophosphorylation GO:0046777 IDA
    intrinsic apoptotic signaling pathway in response to hydrogen peroxide GO:0036481 IGI
    establishment or maintenance of cell polarity GO:0007163 IEA
    response to oxidative stress GO:0006979 TAS
    Golgi reassembly GO:0090168 IMP
    Golgi localization GO:0051645 IDA
    signal transduction by protein phosphorylation GO:0023014 IBA
    response to hydrogen peroxide GO:0042542 IDA
    establishment of Golgi localization GO:0051683 IMP
    signal transduction GO:0007165 TAS
    regulation of apoptotic process GO:0042981 IBA
    regulation of mitotic cell cycle GO:0007346 IBA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    extracellular exosome GO:0070062 IDA
    Golgi apparatus GO:0005794 IDA
 Experiment description of studies that identified STK25 in exosomes
1
Experiment ID 224
ISEV standards
EM|AFM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
CD63|CD81
EV Membrane markers
GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 196
ISEV standards
EM
EV Biophysical techniques
Alix|TSG101|HSP70
EV Cytosolic markers
CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 197
ISEV standards
EM
EV Biophysical techniques
Alix|TSG101|HSP70
EV Cytosolic markers
CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for STK25
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PPP2CA 5515
Affinity Capture-MS Homo sapiens
2 STRN4 29888
Affinity Capture-MS Homo sapiens
3 STRIP1 85369
Affinity Capture-MS Homo sapiens
4 SLMAP  
Affinity Capture-MS Homo sapiens
5 FLJ21168  
Affinity Capture-MS Homo sapiens
6 PDCD10 11235
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
7 TCP1 6950
Affinity Capture-MS Homo sapiens
8 FAM40B  
Affinity Capture-MS Homo sapiens
9 STK24 8428
Affinity Capture-MS Homo sapiens
10 STRN3 29966
Affinity Capture-MS Homo sapiens
11 STRN  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
12 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
13 FGFR1OP2  
Affinity Capture-MS Homo sapiens
14 PPP2R1B 5519
Affinity Capture-MS Homo sapiens
15 DKFZp547A023  
Affinity Capture-MS Homo sapiens
16 PREI3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which STK25 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here