Gene description for AKAP9
Gene name A kinase (PRKA) anchor protein 9
Gene symbol AKAP9
Other names/aliases AKAP-9
AKAP350
AKAP450
CG-NAP
HYPERION
LQT11
MU-RMS-40.16A
PPP1R45
PRKA9
YOTIAO
Species Homo sapiens
 Database cross references - AKAP9
ExoCarta ExoCarta_10142
Vesiclepedia VP_10142
Entrez Gene 10142
HGNC 379
MIM 604001
UniProt Q99996  
 AKAP9 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Malignant pleural effusions 14975938    
 Gene ontology annotations for AKAP9
Molecular Function
    protein complex scaffold GO:0032947 IDA
    protein binding GO:0005515 IPI
    ion channel binding GO:0044325 IPI
    potassium channel regulator activity GO:0015459 IMP
    receptor binding GO:0005102 TAS
Biological Process
    synaptic transmission GO:0007268 TAS
    signal transduction GO:0007165 IEA
    transport GO:0006810 TAS
    regulation of heart rate by cardiac conduction GO:0086091 IMP
    spermatogenesis GO:0007283 IEA
    regulation of ventricular cardiac muscle cell membrane repolarization GO:0060307 IMP
    regulation of membrane repolarization GO:0060306 IMP
    mitotic cell cycle GO:0000278 TAS
    Sertoli cell development GO:0060009 IEA
    positive regulation of potassium ion transmembrane transporter activity GO:1901018 IMP
    cellular response to cAMP GO:0071320 IMP
    organelle organization GO:0006996 TAS
    positive regulation of peptidyl-serine phosphorylation GO:0033138 IMP
    G2/M transition of mitotic cell cycle GO:0000086 TAS
Subcellular Localization
    intracellular membrane-bounded organelle GO:0043231 IDA
    ciliary basal body GO:0036064 IEA
    centrosome GO:0005813 IDA
    voltage-gated potassium channel complex GO:0008076 IDA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 TAS
    pericentriolar material GO:0000242 IEA
 Experiment description of studies that identified AKAP9 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 51
MISEV standards
✔
EM
EV Biophysical techniques
✔
HSP90|MHCII
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 14975938    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes isolated from human malignant pleural effusions.
Authors Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN
Journal name AJRCMB
Publication year 2004
Sample Malignant pleural effusions
Sample name Pleural Fluid - Breast cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
 Protein-protein interactions for AKAP9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TRIP10 9322
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
2 CALM2 805
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
3 PKN1 5585
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
4 PRKAR2A 5576
Protein-peptide Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
5 FNBP1 23048
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
6 CSNK1E 1454
Invitro Homo sapiens
Two-hybrid Homo sapiens
7 CSNK1D 1453
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
8 PPP2R1B 5519
Invitro Homo sapiens
9 CLIC1  
Affinity Capture-Western Homo sapiens
10 KCNQ1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
11 TUBGCP3 10426
Affinity Capture-Western Homo sapiens
12 TACC3  
Two-hybrid Homo sapiens
13 CALM1 801
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
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