Gene description for CDK5RAP2
Gene name CDK5 regulatory subunit associated protein 2
Gene symbol CDK5RAP2
Other names/aliases C48
Cep215
MCPH3
Species Homo sapiens
 Database cross references - CDK5RAP2
ExoCarta ExoCarta_55755
Vesiclepedia VP_55755
Entrez Gene 55755
HGNC 18672
MIM 608201
UniProt Q96SN8  
 CDK5RAP2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Urine 19056867    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for CDK5RAP2
Molecular Function
    transcription cis-regulatory region binding GO:0000976 IDA
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IDA
    microtubule binding GO:0008017 IBA
    microtubule binding GO:0008017 IDA
    tubulin binding GO:0015631 IPI
    protein kinase binding GO:0019901 IPI
    gamma-tubulin binding GO:0043015 IBA
    gamma-tubulin binding GO:0043015 IDA
    protein-containing complex binding GO:0044877 IEA
Biological Process
    establishment of mitotic spindle orientation GO:0000132 IBA
    establishment of mitotic spindle orientation GO:0000132 ISS
    microtubule cytoskeleton organization GO:0000226 IDA
    microtubule bundle formation GO:0001578 IBA
    microtubule bundle formation GO:0001578 IDA
    chromosome segregation GO:0007059 IBA
    chromosome segregation GO:0007059 IMP
    centrosome cycle GO:0007098 IMP
    centriole replication GO:0007099 IBA
    centriole replication GO:0007099 IMP
    brain development GO:0007420 ISS
    brain development GO:0007420 NAS
    neurogenesis GO:0022008 ISS
    microtubule organizing center organization GO:0031023 IMP
    positive regulation of microtubule polymerization GO:0031116 IMP
    regulation of neuron differentiation GO:0045664 NAS
    negative regulation of neuron differentiation GO:0045665 IEA
    positive regulation of DNA-templated transcription GO:0045893 IDA
    negative regulation of centriole replication GO:0046600 IBA
    negative regulation of centriole replication GO:0046600 ISS
    regulation of mitotic cell cycle spindle assembly checkpoint GO:0090266 IBA
    regulation of mitotic cell cycle spindle assembly checkpoint GO:0090266 IDA
Subcellular Localization
    pericentriolar material GO:0000242 IBA
    pericentriolar material GO:0000242 IDA
    spindle pole GO:0000922 IDA
    gamma-tubulin ring complex GO:0000931 IDA
    cytoplasm GO:0005737 IDA
    Golgi apparatus GO:0005794 IDA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 NAS
    microtubule GO:0005874 IDA
    cell junction GO:0030054 IDA
    microtubule plus-end GO:0035371 IBA
    microtubule plus-end GO:0035371 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    extracellular exosome GO:0070062 HDA
    mitotic spindle pole GO:0097431 IBA
 Experiment description of studies that identified CDK5RAP2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
7
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CDK5RAP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KRT37 8688
Affinity Capture-MS Homo sapiens
2 PRELID2  
Affinity Capture-MS Homo sapiens
3 BIRC6 57448
Proximity Label-MS Homo sapiens
4 LINC01587  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 USP43  
Affinity Capture-MS Homo sapiens
6 EEF1G 1937
Two-hybrid Homo sapiens
7 BUB1  
Proximity Label-MS Homo sapiens
8 ALMS1  
Proximity Label-MS Homo sapiens
9 S100A6 6277
Affinity Capture-MS Homo sapiens
10 TPTE2  
Proximity Label-MS Homo sapiens
11 FBXO25  
Two-hybrid Homo sapiens
12 KIF20A 10112
Affinity Capture-MS Homo sapiens
13 NOBOX  
Affinity Capture-MS Homo sapiens
14 CALM1 801
Affinity Capture-MS Homo sapiens
15 STK36  
Two-hybrid Homo sapiens
16 ZAR1L  
Affinity Capture-MS Homo sapiens
17 HDAC1 3065
Affinity Capture-MS Homo sapiens
18 NEDD1 121441
Proximity Label-MS Homo sapiens
19 RBM48  
Two-hybrid Homo sapiens
20 C1orf87  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 PCNT  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
22 NME7 29922
Affinity Capture-MS Homo sapiens
23 TSNAX 7257
Two-hybrid Homo sapiens
24 MAGEH1  
Two-hybrid Homo sapiens
25 CCDC14  
Proximity Label-MS Homo sapiens
26 PCBD1 5092
Two-hybrid Homo sapiens
27 CEP112  
Proximity Label-MS Homo sapiens
28 PRC1 9055
Affinity Capture-MS Homo sapiens
29 KRT40  
Affinity Capture-MS Homo sapiens
30 Fgfr1op  
Affinity Capture-MS Mus musculus
31 PRPS1 5631
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 ABCA2 20
Two-hybrid Homo sapiens
33 LRRC61 65999
Proximity Label-MS Homo sapiens
34 DYNLL1 8655
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 CRMP1 1400
Two-hybrid Homo sapiens
36 TUBGCP6 85378
Affinity Capture-MS Homo sapiens
37 CCDC18  
Proximity Label-MS Homo sapiens
38 DNAJC7 7266
Proximity Label-MS Homo sapiens
39 HAP1  
Two-hybrid Homo sapiens
40 POLR2C 5432
Proximity Label-MS Homo sapiens
41 LPXN 9404
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 MUS81  
Synthetic Growth Defect Homo sapiens
43 KNSTRN  
Affinity Capture-MS Homo sapiens
44 CEP131 22994
Proximity Label-MS Homo sapiens
45 Spag5  
Affinity Capture-MS Mus musculus
46 TEX9  
Proximity Label-MS Homo sapiens
47 KIF14 9928
Affinity Capture-MS Homo sapiens
48 C15orf59  
Affinity Capture-MS Homo sapiens
49 KRT8 3856
Proximity Label-MS Homo sapiens
50 CEP350 9857
Proximity Label-MS Homo sapiens
51 SENP2 59343
Two-hybrid Homo sapiens
52 MAGED2 10916
Proximity Label-MS Homo sapiens
53 IKBIP 121457
Affinity Capture-MS Homo sapiens
54 SOCS6  
Affinity Capture-MS Homo sapiens
55 PIBF1  
Proximity Label-MS Homo sapiens
56 CDK5 1020
Reconstituted Complex Homo sapiens
57 CEP192 55125
Proximity Label-MS Homo sapiens
58 FAM9C  
Affinity Capture-MS Homo sapiens
59 MAPRE1 22919
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
60 PRPS2 5634
Affinity Capture-MS Homo sapiens
61 SSX2  
Affinity Capture-MS Homo sapiens
62 OFD1 8481
Proximity Label-MS Homo sapiens
63 DNAJB8  
Proximity Label-MS Homo sapiens
64 TRIM37  
Two-hybrid Homo sapiens
65 DTNBP1  
Affinity Capture-MS Homo sapiens
66 CEP152  
Proximity Label-MS Homo sapiens
67 CHMP4B 128866
Affinity Capture-MS Homo sapiens
68 FYN 2534
Affinity Capture-Western Homo sapiens
69 CARD9 64170
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 CDK5R1  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
71 LUC7L2 51631
Two-hybrid Homo sapiens
72 DHFRL1  
Proximity Label-MS Homo sapiens
73 STIL  
Proximity Label-MS Homo sapiens
74 DYNLL2 140735
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 AGPS 8540
Proximity Label-MS Homo sapiens
76 DNAJB5  
Proximity Label-MS Homo sapiens
77 PRKACB 5567
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 FGFR1OP  
Affinity Capture-MS Homo sapiens
79 PIPSL 266971
Affinity Capture-MS Homo sapiens
80 SSX2IP  
Proximity Label-MS Homo sapiens
81 ZPR1 8882
Two-hybrid Homo sapiens
82 NCKAP5L  
Proximity Label-MS Homo sapiens
83 PTN  
Two-hybrid Homo sapiens
84 CEP57  
Affinity Capture-MS Homo sapiens
85 WHAMMP3  
Affinity Capture-MS Homo sapiens
86 TUBGCP5 114791
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
87 PXN 5829
Proximity Label-MS Homo sapiens
88 C14orf1 11161
Two-hybrid Homo sapiens
89 AKAP9 10142
Proximity Label-MS Homo sapiens
90 PRKAR2A 5576
Proximity Label-MS Homo sapiens
91 C11orf49  
Proximity Label-MS Homo sapiens
92 MZT2B 80097
Affinity Capture-MS Homo sapiens
93 NR3C1 2908
Proximity Label-MS Homo sapiens
94 CCP110  
Proximity Label-MS Homo sapiens
95 RPGRIP1L 23322
Proximity Label-MS Homo sapiens
96 CTAG2  
Affinity Capture-MS Homo sapiens
97 DUSP16  
Affinity Capture-MS Homo sapiens
98 SUZ12  
Affinity Capture-MS Homo sapiens
99 SASS6 163786
Proximity Label-MS Homo sapiens
100 SSH3 54961
Proximity Label-MS Homo sapiens
101 AURKB 9212
Affinity Capture-MS Homo sapiens
102 MLF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 MAPRE3  
Proximity Label-MS Homo sapiens
104 PRKACA 5566
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
105 Uso1 56041
Affinity Capture-MS Mus musculus
106 TXNL1 9352
Proximity Label-MS Homo sapiens
107 KAT5  
Two-hybrid Homo sapiens
108 NDC80 10403
Proximity Label-MS Homo sapiens
109 DNAJB13  
Proximity Label-MS Homo sapiens
110 PCM1 5108
Proximity Label-MS Homo sapiens
111 HAX1  
Proximity Label-MS Homo sapiens
112 KRT19 3880
Proximity Label-MS Homo sapiens
113 Prkar2a 19087
Affinity Capture-MS Mus musculus
114 MZT2A  
Affinity Capture-MS Homo sapiens
115 Prkacb 18749
Affinity Capture-MS Mus musculus
116 ACBD5 91452
Proximity Label-MS Homo sapiens
117 TNNT1  
Affinity Capture-MS Homo sapiens
118 RALBP1 10928
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
119 TUBGCP2 10844
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
120 STARD9 57519
Two-hybrid Homo sapiens
121 CEP68 23177
Affinity Capture-Western Homo sapiens
122 TRIM52  
Affinity Capture-MS Homo sapiens
123 TUBG1 7283
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
124 TUBGCP3 10426
Affinity Capture-MS Homo sapiens
125 SEPT10 151011
Proximity Label-MS Homo sapiens
126 PTTG1  
Synthetic Growth Defect Homo sapiens
127 DCTN2 10540
Proximity Label-MS Homo sapiens
128 CDK5R1  
Two-hybrid Bos taurus
129 DDX58 23586
Affinity Capture-RNA Homo sapiens
130 PRKAR2B 5577
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
131 LATS1  
Proximity Label-MS Homo sapiens
132 FGL2 10875
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
133 ANAPC2 29882
Proximity Label-MS Homo sapiens
134 MIF 4282
Affinity Capture-MS Homo sapiens
135 C6orf141  
Affinity Capture-MS Homo sapiens
136 FAM81B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
137 STXBP4 252983
Proximity Label-MS Homo sapiens
138 Mapre1 13589
Affinity Capture-MS Mus musculus
139 KRT38 8687
Affinity Capture-MS Homo sapiens
140 TRIM36  
Proximity Label-MS Homo sapiens
141 Prkar2b 19088
Affinity Capture-MS Mus musculus
142 CEP135  
Proximity Label-MS Homo sapiens
143 PRDX1 5052
Cross-Linking-MS (XL-MS) Homo sapiens
144 CIT 11113
Affinity Capture-MS Homo sapiens
145 PDE4DIP 9659
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
146 CCDC96  
Affinity Capture-MS Homo sapiens
147 LRRC49  
Proximity Label-MS Homo sapiens
148 RPA2 6118
Proximity Label-MS Homo sapiens
149 LURAP1  
Affinity Capture-MS Homo sapiens
150 C17orf59 54785
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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