Gene description for SSH3
Gene name slingshot protein phosphatase 3
Gene symbol SSH3
Other names/aliases SSH3L
Species Homo sapiens
 Database cross references - SSH3
ExoCarta ExoCarta_54961
Vesiclepedia VP_54961
Entrez Gene 54961
HGNC 30581
MIM 606780
UniProt Q8TE77  
 SSH3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for SSH3
Molecular Function
    actin binding GO:0003779 IBA
    phosphoprotein phosphatase activity GO:0004721 IBA
    non-membrane spanning protein tyrosine phosphatase activity GO:0004726 IEA
    protein binding GO:0005515 IPI
    myosin phosphatase activity GO:0017018 IEA
    protein tyrosine phosphatase activity, metal-dependent GO:0030946 IEA
    calmodulin-dependent protein phosphatase activity GO:0033192 IEA
    histone H2AXS140 phosphatase activity GO:0140791 IEA
    histone H2AXY142 phosphatase activity GO:0140793 IEA
    RNA polymerase II CTD heptapeptide repeat Y1 phosphatase activity GO:0180004 IEA
    RNA polymerase II CTD heptapeptide repeat T4 phosphatase activity GO:0180005 IEA
    RNA polymerase II CTD heptapeptide repeat S2 phosphatase activity GO:0180006 IEA
    RNA polymerase II CTD heptapeptide repeat S5 phosphatase activity GO:0180007 IEA
    RNA polymerase II CTD heptapeptide repeat S7 phosphatase activity GO:0180008 IEA
    MAP kinase serine/threonine phosphatase activity GO:1990439 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    actin cytoskeleton organization GO:0030036 IBA
    negative regulation of actin filament polymerization GO:0030837 IBA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IBA
    cytoskeleton GO:0005856 IEA
 Experiment description of studies that identified SSH3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SSH3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HERC1 8925
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 RELA 5970
Proximity Label-MS Homo sapiens
3 KIAA0232 9778
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 SPATA5L1 79029
Affinity Capture-MS Homo sapiens
5 SLC4A1AP  
Affinity Capture-MS Homo sapiens
6 CPT2 1376
Affinity Capture-MS Homo sapiens
7 JMJD4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 MKS1 54903
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 C7orf55  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 HSPA8 3312
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 PSME3 10197
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 PCMT1 5110
Affinity Capture-MS Homo sapiens
13 CALU 813
Affinity Capture-MS Homo sapiens
14 RPA2 6118
Co-fractionation Homo sapiens
15 TMEM248 55069
Affinity Capture-MS Homo sapiens
16 CDK5RAP2 55755
Proximity Label-MS Homo sapiens
17 RPA1 6117
Co-fractionation Homo sapiens
18 KEAP1 9817
Affinity Capture-MS Homo sapiens
19 RFXANK  
Affinity Capture-MS Homo sapiens
20 CAPZA2 830
Co-fractionation Homo sapiens
21 ZNF330  
Affinity Capture-MS Homo sapiens
22 OTUB1 55611
Affinity Capture-MS Homo sapiens
23 SHMT2 6472
Affinity Capture-RNA Homo sapiens
24 NBR1 4077
Proximity Label-MS Homo sapiens
25 APP 351
Reconstituted Complex Homo sapiens
26 SSH3 54961
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 POLA2  
Affinity Capture-MS Homo sapiens
28 APEX1 328
Affinity Capture-RNA Homo sapiens
29 CECR5 27440
Affinity Capture-MS Homo sapiens
30 FAM162A 26355
Affinity Capture-MS Homo sapiens
31 BIRC6 57448
Proximity Label-MS Homo sapiens
32 PJA1  
Affinity Capture-MS Homo sapiens
33 CDH1 999
Proximity Label-MS Homo sapiens
34 INF2 64423
Affinity Capture-MS Homo sapiens
35 EIF4B 1975
Co-fractionation Homo sapiens
36 UHRF1BP1 54887
Affinity Capture-MS Homo sapiens
37 SORT1 6272
Affinity Capture-MS Homo sapiens
38 TRIM35 23087
Affinity Capture-MS Homo sapiens
39 LIPA 3988
Co-fractionation Homo sapiens
40 METTL21B  
Affinity Capture-MS Homo sapiens
41 CCT8L1P  
Affinity Capture-MS Homo sapiens
42 CAMK2D 817
Affinity Capture-MS Homo sapiens
43 CRYBG3  
Proximity Label-MS Homo sapiens
44 C2orf47  
Affinity Capture-MS Homo sapiens
45 YBEY  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 ANGEL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 STIP1 10963
Affinity Capture-MS Homo sapiens
48 SF3B3 23450
Affinity Capture-MS Homo sapiens
49 KDM5C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 PFAS 5198
Proximity Label-MS Homo sapiens
51 PARN  
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which SSH3 is involved
No pathways found





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