Gene description for TRIM35
Gene name tripartite motif containing 35
Gene symbol TRIM35
Other names/aliases HLS5
MAIR
Species Homo sapiens
 Database cross references - TRIM35
ExoCarta ExoCarta_23087
Vesiclepedia VP_23087
Entrez Gene 23087
HGNC 16285
UniProt Q9UPQ4  
 TRIM35 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for TRIM35
Molecular Function
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
    ubiquitin protein ligase activity GO:0061630 IBA
Biological Process
    apoptotic process GO:0006915 IEA
    protein ubiquitination GO:0016567 IEA
    positive regulation of apoptotic process GO:0043065 IBA
    positive regulation of apoptotic process GO:0043065 IDA
    suppression of viral release by host GO:0044790 IDA
    innate immune response GO:0045087 IBA
    innate immune response GO:0045087 IDA
    negative regulation of mitotic cell cycle GO:0045930 IDA
Subcellular Localization
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISS
 Experiment description of studies that identified TRIM35 in exosomes
1
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for TRIM35
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CHMP2A 27243
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 ABCF3 55324
Affinity Capture-MS Homo sapiens
3 ENKD1  
Two-hybrid Homo sapiens
4 VPS41 27072
Affinity Capture-MS Homo sapiens
5 GNMT 27232
Two-hybrid Homo sapiens
6 CDC42BPB 9578
Affinity Capture-MS Homo sapiens
7 QARS 5859
Two-hybrid Homo sapiens
8 UBE2K 3093
Two-hybrid Homo sapiens
9 PKM 5315
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
10 MYO1F 4542
Affinity Capture-MS Homo sapiens
11 MGAT5B  
Two-hybrid Homo sapiens
12 TSG101 7251
Two-hybrid Homo sapiens
13 TCF7L2  
Affinity Capture-MS Homo sapiens
14 OTUD5 55593
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 SAMD1  
Affinity Capture-MS Homo sapiens
16 TRIM55  
Two-hybrid Homo sapiens
17 CABP2  
Two-hybrid Homo sapiens
18 PPP2R1B 5519
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 UBE2D4  
Two-hybrid Homo sapiens
20 APAF1 317
Affinity Capture-MS Homo sapiens
21 WDR20 91833
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 PRKAB2 5565
Two-hybrid Homo sapiens
23 COL8A1 1295
Two-hybrid Homo sapiens
24 IGFN1  
Two-hybrid Homo sapiens
25 VWA5B2  
Affinity Capture-MS Homo sapiens
26 UBE2W  
Two-hybrid Homo sapiens
27 USP2  
Two-hybrid Homo sapiens
28 AKTIP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 USP7 7874
Affinity Capture-MS Homo sapiens
30 PRR20C  
Two-hybrid Homo sapiens
31 MTA2 9219
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 PRR20A  
Two-hybrid Homo sapiens
33 BANP  
Two-hybrid Homo sapiens
34 ANTXR1 84168
Two-hybrid Homo sapiens
35 SAE1 10055
Affinity Capture-MS Homo sapiens
36 PDXDC1 23042
Affinity Capture-MS Homo sapiens
37 PAN2  
Two-hybrid Homo sapiens
38 PDCD5 9141
Two-hybrid Homo sapiens
39 TSSK3  
Two-hybrid Homo sapiens
40 FOXK2  
Affinity Capture-MS Homo sapiens
41 TRAPPC2L 51693
Affinity Capture-MS Homo sapiens
42 PPP2CA 5515
Two-hybrid Homo sapiens
43 TRIM11  
Two-hybrid Homo sapiens
44 UBE2D3 7323
Two-hybrid Homo sapiens
45 ARAP3 64411
Affinity Capture-MS Homo sapiens
46 TRIM63  
Two-hybrid Homo sapiens
47 NRF1 4899
Two-hybrid Homo sapiens
48 TRIM10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
49 HSPA14 51182
Affinity Capture-MS Homo sapiens
50 PRR20D  
Two-hybrid Homo sapiens
51 SSH3 54961
Affinity Capture-MS Homo sapiens
52 UBE2D2 7322
Two-hybrid Homo sapiens
53 PRR20B  
Two-hybrid Homo sapiens
54 PIAS1 8554
Affinity Capture-MS Homo sapiens
55 RGPD5  
Affinity Capture-MS Homo sapiens
56 DIS3 22894
Affinity Capture-MS Homo sapiens
57 ZNF410  
Two-hybrid Homo sapiens
58 TIGAR 57103
Affinity Capture-Western Homo sapiens
59 SHC3  
Two-hybrid Homo sapiens
60 TCEANC  
Two-hybrid Homo sapiens
61 ZMYM2  
Affinity Capture-MS Homo sapiens
62 UBE2U  
Two-hybrid Homo sapiens
63 KIAA1045 23349
Two-hybrid Homo sapiens
64 UBE2D1 7321
Two-hybrid Homo sapiens
65 RGL3  
Affinity Capture-MS Homo sapiens
66 TRIM5 85363
Reconstituted Complex Homo sapiens
67 KIF1A 547
Affinity Capture-MS Homo sapiens
68 USP49 25862
Two-hybrid Homo sapiens
69 RAD17  
Affinity Capture-MS Homo sapiens
70 USP34 9736
Affinity Capture-MS Homo sapiens
71 MKS1 54903
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 RNF31 55072
Affinity Capture-MS Homo sapiens
73 CHTF18 63922
Affinity Capture-MS Homo sapiens
74 HNRNPA2B1 3181
Two-hybrid Homo sapiens
75 LCK 3932
Two-hybrid Homo sapiens
76 DNAAF5 54919
Affinity Capture-MS Homo sapiens
77 OXSR1 9943
Affinity Capture-MS Homo sapiens
78 KLHL11  
Affinity Capture-MS Homo sapiens
79 VPS33A 65082
Affinity Capture-MS Homo sapiens
80 ADIPOQ 9370
Two-hybrid Homo sapiens
81 PRR20E  
Two-hybrid Homo sapiens
82 PARD6B 84612
Two-hybrid Homo sapiens
83 NAB2 4665
Affinity Capture-MS Homo sapiens
84 KANK2 25959
Two-hybrid Homo sapiens
85 EIF2AK2 5610
Affinity Capture-MS Homo sapiens
86 LIMD1 8994
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 LMO1  
Two-hybrid Homo sapiens
88 PPP2R2A 5520
Affinity Capture-MS Homo sapiens
89 LIMK2 3985
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 PPP2R2D 55844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 CDK11A 728642
Affinity Capture-MS Homo sapiens
92 KDM5C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TRIM35 is involved
PathwayEvidenceSource
Cytokine Signaling in Immune system TAS Reactome
Immune System TAS Reactome
Interferon gamma signaling TAS Reactome
Interferon Signaling TAS Reactome





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