Gene description for ABCF3
Gene name ATP-binding cassette, sub-family F (GCN20), member 3
Gene symbol ABCF3
Other names/aliases EST201864
Species Homo sapiens
 Database cross references - ABCF3
ExoCarta ExoCarta_55324
Vesiclepedia VP_55324
Entrez Gene 55324
HGNC 72
UniProt Q9NUQ8  
 ABCF3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Mesenchymal stem cells 36408942    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for ABCF3
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IBA
    ATP hydrolysis activity GO:0016887 IEA
    cadherin binding GO:0045296 HDA
Biological Process
    defense response to virus GO:0051607 IEA
Subcellular Localization
    membrane GO:0016020 HDA
 Experiment description of studies that identified ABCF3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
11
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ABCF3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATP6V1B2 526
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
2 KLK15  
Affinity Capture-MS Homo sapiens
3 CIB2  
Affinity Capture-MS Homo sapiens
4 S100A6 6277
Affinity Capture-MS Homo sapiens
5 Skp1a 21402
Affinity Capture-MS Mus musculus
6 MID1IP1  
Affinity Capture-MS Homo sapiens
7 RFXANK  
Affinity Capture-MS Homo sapiens
8 PACSIN2 11252
Affinity Capture-MS Homo sapiens
9 TRIM27  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
10 DCTN3 11258
Affinity Capture-MS Homo sapiens
11 LAMTOR1 55004
Two-hybrid Homo sapiens
12 RPS20 6224
Proximity Label-MS Homo sapiens
13 BTLA  
Affinity Capture-MS Homo sapiens
14 D2HGDH 728294
Affinity Capture-MS Homo sapiens
15 FAM57A  
Affinity Capture-MS Homo sapiens
16 SRRM2 23524
Affinity Capture-MS Homo sapiens
17 DDRGK1 65992
Affinity Capture-MS Homo sapiens
18 ELSPBP1  
Affinity Capture-MS Homo sapiens
19 NPAS1  
Affinity Capture-MS Homo sapiens
20 YPEL3  
Affinity Capture-MS Homo sapiens
21 KIF14 9928
Affinity Capture-MS Homo sapiens
22 RPS6KL1  
Affinity Capture-MS Homo sapiens
23 FAM96A  
Affinity Capture-MS Homo sapiens
24 SPTB 6710
Affinity Capture-MS Homo sapiens
25 PLBD1 79887
Affinity Capture-MS Homo sapiens
26 PARK2  
Affinity Capture-MS Homo sapiens
27 CCDC33 80125
Two-hybrid Homo sapiens
28 ACIN1 22985
Two-hybrid Homo sapiens
29 ABCF2 10061
Co-fractionation Homo sapiens
30 CDH1 999
Proximity Label-MS Homo sapiens
31 NTRK1 4914
Affinity Capture-MS Homo sapiens
32 PPARD  
Affinity Capture-MS Homo sapiens
33 TEAD2  
Affinity Capture-MS Homo sapiens
34 DCTN1 1639
Proximity Label-MS Homo sapiens
35 TPP2 7174
Affinity Capture-MS Homo sapiens
36 MTMR4  
Affinity Capture-MS Homo sapiens
37 HSPB8 26353
Affinity Capture-MS Homo sapiens
38 UBXN6 80700
Affinity Capture-MS Homo sapiens
39 USP32 84669
Affinity Capture-MS Homo sapiens
40 PSG11  
Affinity Capture-MS Homo sapiens
41 ZBED6CL  
Affinity Capture-MS Homo sapiens
42 DLEU1  
Two-hybrid Homo sapiens
43 HSPA5 3309
Affinity Capture-MS Homo sapiens
44 AKAP9 10142
Affinity Capture-MS Homo sapiens
45 CTNNB1 1499
Affinity Capture-MS Homo sapiens
46 ASB6 140459
Affinity Capture-MS Homo sapiens
47 PDE4D  
Affinity Capture-MS Homo sapiens
48 TRIM35 23087
Affinity Capture-MS Homo sapiens
49 FBXO6 26270
Affinity Capture-MS Homo sapiens
50 FOXL1  
Affinity Capture-MS Homo sapiens
51 METTL21B  
Affinity Capture-MS Homo sapiens
52 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
53 NXF1 10482
Affinity Capture-RNA Homo sapiens
54 C12orf74  
Affinity Capture-MS Homo sapiens
55 PPP2R1A 5518
Affinity Capture-MS Homo sapiens
56 DNAI2  
Affinity Capture-MS Homo sapiens
57 ARL4D  
Affinity Capture-MS Homo sapiens
58 NLRP9  
Co-fractionation Homo sapiens
59 FCHSD2  
Two-hybrid Homo sapiens
60 CLASRP  
Affinity Capture-MS Homo sapiens
61 SIRT6  
Affinity Capture-MS Homo sapiens
62 LGI1  
Affinity Capture-MS Homo sapiens
63 RNF2  
Affinity Capture-MS Homo sapiens
64 CDKN1B 1027
Co-fractionation Homo sapiens
65 FSCN1 6624
Affinity Capture-MS Homo sapiens
66 PIP5KL1  
Affinity Capture-MS Homo sapiens
67 NES 10763
Affinity Capture-MS Homo sapiens
68 TARSL2 123283
Affinity Capture-MS Homo sapiens
69 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
70 DNALI1  
Two-hybrid Homo sapiens
71 TOE1 114034
Two-hybrid Homo sapiens
72 PRKY  
Affinity Capture-MS Homo sapiens
73 MTMR3  
Affinity Capture-MS Homo sapiens
74 HDAC6 10013
Affinity Capture-MS Homo sapiens
75 KCTD14 65987
Affinity Capture-MS Homo sapiens
76 SAP18 10284
Affinity Capture-MS Homo sapiens
77 INTS2  
Co-fractionation Homo sapiens
78 FYCO1 79443
Affinity Capture-MS Homo sapiens
79 CNOT3 4849
Affinity Capture-MS Homo sapiens
80 GSTM3 2947
Affinity Capture-MS Homo sapiens
81 DHFR 1719
Affinity Capture-MS Homo sapiens
82 RIOK2 55781
Affinity Capture-MS Homo sapiens
83 MOV10 4343
Affinity Capture-RNA Homo sapiens
84 UPF1 5976
Co-fractionation Homo sapiens
85 SSSCA1 10534
Affinity Capture-MS Homo sapiens
86 HNRNPF 3185
Co-fractionation Homo sapiens
87 SNW1 22938
Affinity Capture-MS Homo sapiens
88 MAP1A 4130
Affinity Capture-MS Homo sapiens
89 CCT8L2  
Affinity Capture-MS Homo sapiens
90 RPA3 6119
Proximity Label-MS Homo sapiens
91 EP300 2033
Affinity Capture-MS Homo sapiens
92 CDC5L 988
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ABCF3 is involved
No pathways found





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