Gene description for SPTB
Gene name spectrin, beta, erythrocytic
Gene symbol SPTB
Other names/aliases EL3
HS2
HSPTB1
SPH2
Species Homo sapiens
 Database cross references - SPTB
ExoCarta ExoCarta_6710
Vesiclepedia VP_6710
Entrez Gene 6710
HGNC 11274
MIM 182870
UniProt P11277  
 SPTB identified in sEVs derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for SPTB
Molecular Function
    actin binding GO:0003779 TAS
    structural constituent of cytoskeleton GO:0005200 IEA
    protein binding GO:0005515 IPI
    ankyrin binding GO:0030506 IPI
    actin filament binding GO:0051015 IBA
    actin filament binding GO:0051015 IDA
Biological Process
    actin cytoskeleton organization GO:0030036 IBA
    actin filament capping GO:0051693 IEA
    modification of postsynaptic actin cytoskeleton GO:0098885 IDA
    modification of postsynaptic actin cytoskeleton GO:0098885 IMP
Subcellular Localization
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    spectrin GO:0008091 IEA
    cytoplasmic side of plasma membrane GO:0009898 TAS
    cell surface GO:0009986 IDA
    spectrin-associated cytoskeleton GO:0014731 IDA
    actin cytoskeleton GO:0015629 TAS
    cell junction GO:0030054 IBA
    cortical actin cytoskeleton GO:0030864 IBA
    protein-containing complex GO:0032991 IDA
    cell projection GO:0042995 IBA
    postsynapse GO:0098794 IEA
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
 Experiment description of studies that identified SPTB in sEVs
1
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SPTB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NCAM1 4684
Reconstituted Complex Homo sapiens
2 ANLN 54443
Affinity Capture-MS Homo sapiens
3 ABCF3 55324
Affinity Capture-MS Homo sapiens
4 OR13C5  
Affinity Capture-MS Homo sapiens
5 GAN 8139
Affinity Capture-MS Homo sapiens
6 YARS2  
Affinity Capture-MS Homo sapiens
7 SPTAN1 6709
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
8 C10orf53  
Affinity Capture-MS Homo sapiens
9 KIF14 9928
Affinity Capture-MS Homo sapiens
10 SLU7  
Affinity Capture-MS Homo sapiens
11 ACOT6  
Affinity Capture-MS Homo sapiens
12 CHTOP  
Affinity Capture-MS Homo sapiens
13 RAB40AL  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 EPB41 2035
Reconstituted Complex Homo sapiens
15 KIF20A 10112
Affinity Capture-MS Homo sapiens
16 ANK1 286
Reconstituted Complex Homo sapiens
17 ECT2 1894
Affinity Capture-MS Homo sapiens
18 BLOC1S5-TXNDC5  
Affinity Capture-MS Homo sapiens
19 GARS 2617
Affinity Capture-MS Homo sapiens
20 CHMP4C 92421
Affinity Capture-MS Homo sapiens
21 BTF3 689
Affinity Capture-MS Homo sapiens
22 ZBTB12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 RPRD2  
Affinity Capture-MS Homo sapiens
24 SPTA1  
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
25 CIT 11113
Affinity Capture-MS Homo sapiens
26 GLB1L2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 RASA3 22821
Co-fractionation Homo sapiens
28 BOLA1 51027
Affinity Capture-MS Homo sapiens
29 LSM5  
Co-fractionation Homo sapiens
30 PAPOLG  
Affinity Capture-MS Homo sapiens
31 SOX2  
Affinity Capture-MS Homo sapiens
32 NMNAT3 349565
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 IL13 3596
Affinity Capture-MS Homo sapiens
34 FAM107A  
Affinity Capture-MS Homo sapiens
35 CHMP4B 128866
Affinity Capture-MS Homo sapiens
36 RNF145  
Affinity Capture-MS Homo sapiens
37 CDK9 1025
Affinity Capture-MS Homo sapiens
38 MTURN  
Affinity Capture-MS Homo sapiens
39 CPLX4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 SERPINB4 6318
Affinity Capture-MS Homo sapiens
41 CRP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 DIDO1  
Affinity Capture-MS Homo sapiens
43 CLSTN2 64084
Affinity Capture-MS Homo sapiens
44 PRC1 9055
Affinity Capture-MS Homo sapiens
45 YWHAQ 10971
Reconstituted Complex Homo sapiens
46 RHOD 29984
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 ACTB 60
Reconstituted Complex Homo sapiens
48 CDKN2AIPNL  
Affinity Capture-MS Homo sapiens
49 TXNDC5 81567
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 ADD1 118
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
51 MTFR2  
Affinity Capture-MS Homo sapiens
52 MRPL38  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 CABP4  
Affinity Capture-MS Homo sapiens
54 FTSJ3 117246
Affinity Capture-MS Homo sapiens
55 LOC388882  
Affinity Capture-MS Homo sapiens
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