Gene description for SPTB
Gene name spectrin, beta, erythrocytic
Gene symbol SPTB
Other names/aliases EL3
HS2
HSPTB1
SPH2
Species Homo sapiens
 Database cross references - SPTB
ExoCarta ExoCarta_6710
Vesiclepedia VP_6710
Entrez Gene 6710
HGNC 11274
MIM 182870
UniProt P11277  
 SPTB identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for SPTB
Molecular Function
    protein binding GO:0005515 IPI
    protein heterodimerization activity GO:0046982 IEA
    structural constituent of cytoskeleton GO:0005200 IEA
    ankyrin binding GO:0030506 IPI
    actin filament binding GO:0051015 IDA
    actin binding GO:0003779 TAS
Biological Process
    hemopoiesis GO:0030097 IEA
    axon guidance GO:0007411 TAS
    actin filament capping GO:0051693 IEA
    porphyrin-containing compound biosynthetic process GO:0006779 IEA
    plasma membrane organization GO:0007009 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
    actin cytoskeleton GO:0015629 TAS
    cytoplasm GO:0005737 IDA
    protein complex GO:0043234 IDA
    spectrin GO:0008091 IEA
    intrinsic component of the cytoplasmic side of the plasma membrane GO:0031235 TAS
    spectrin-associated cytoskeleton GO:0014731 IDA
    cell surface GO:0009986 IDA
 Experiment description of studies that identified SPTB in exosomes
1
Experiment ID 224
MISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101|CD63|CD81
EV Enriched markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 231
MISEV standards
✘ EV Biophysical techniques
✔
Alix|CD63|CD9
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 232
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 233
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SPTB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AP1G1 164
Two-hybrid Homo sapiens
2 ANK1 286
Reconstituted Complex Homo sapiens
3 ADD1 118
Invitro Homo sapiens
4 SPTAN1 6709
Invivo Homo sapiens
5 MAPT  
Invivo Homo sapiens
6 SPTA1  
Invitro Homo sapiens
7 EPB41 2035
Invivo Homo sapiens
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which SPTB is involved
PathwayEvidenceSource
Interaction between L1 and Ankyrins TAS Reactome
NCAM signaling for neurite out-growth TAS Reactome





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