Gene description for ARAP3
Gene name ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3
Gene symbol ARAP3
Other names/aliases CENTD3
DRAG1
Species Homo sapiens
 Database cross references - ARAP3
ExoCarta ExoCarta_64411
Entrez Gene 64411
HGNC 24097
MIM 606647
UniProt Q8WWN8  
 ARAP3 identified in exosomes derived from the following tissue/cell type
Squamous carcinoma cells 20124223    
 Gene ontology annotations for ARAP3
Molecular Function
    phosphatidylinositol-3,4-bisphosphate binding GO:0043325 IDA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    GTPase activator activity GO:0005096 ISS
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 IDA
Biological Process
    negative regulation of cell migration GO:0030336 ISS
    vesicle-mediated transport GO:0016192 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    positive regulation of GTPase activity GO:0043547 IEA
    negative regulation of Rho protein signal transduction GO:0035024 ISS
    negative regulation of Rac protein signal transduction GO:0035021 IEA
    regulation of cell shape GO:0008360 ISS
    cytoskeleton organization GO:0007010 TAS
Subcellular Localization
    lamellipodium GO:0030027 ISS
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IEA
    ruffle GO:0001726 ISS
    plasma membrane GO:0005886 IEA
    cytoplasm GO:0005737 ISS
 Experiment description of studies that identified ARAP3 in exosomes
1
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ARAP3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which ARAP3 is involved
PathwayEvidenceSource
Rho GTPase cycle TAS Reactome





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