Gene description for NT5C
Gene name 5', 3'-nucleotidase, cytosolic
Gene symbol NT5C
Other names/aliases DNT
DNT1
HEL74
P5N2
PN-I
PN-II
UMPH2
cdN
dNT-1
Species Homo sapiens
 Database cross references - NT5C
ExoCarta ExoCarta_30833
Vesiclepedia VP_30833
Entrez Gene 30833
HGNC 17144
MIM 191720
UniProt Q8TCD5  
 NT5C identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Urine 19056867    
 Gene ontology annotations for NT5C
Molecular Function
    nucleotidase activity GO:0008252 IDA
    5'-nucleotidase activity GO:0008253 IBA
    pyrimidine nucleotide binding GO:0019103 IDA
    identical protein binding GO:0042802 IPI
    metal ion binding GO:0046872 IEA
    IMP 5'-nucleotidase activity GO:0050483 IEA
Biological Process
    allantoin metabolic process GO:0000255 IEA
    IMP catabolic process GO:0006204 IEA
    dCMP catabolic process GO:0006249 IEA
    pyrimidine deoxyribonucleotide catabolic process GO:0009223 IBA
    pyrimidine deoxyribonucleotide catabolic process GO:0009223 IDA
    dephosphorylation GO:0016311 IDA
    amide catabolic process GO:0043605 IEA
    UMP catabolic process GO:0046050 IEA
    dGMP catabolic process GO:0046055 IEA
    dTMP catabolic process GO:0046074 IEA
    dUMP catabolic process GO:0046079 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 HTP
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified NT5C in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for NT5C
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NEUROG1  
Affinity Capture-MS Homo sapiens
2 SHKBP1  
Affinity Capture-MS Homo sapiens
3 APAF1 317
Affinity Capture-MS Homo sapiens
4 ASCC3 10973
Affinity Capture-MS Homo sapiens
5 RAP1GDS1 5910
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 RGL3  
Affinity Capture-MS Homo sapiens
7 IFT140 9742
Affinity Capture-MS Homo sapiens
8 PLEKHH3  
Affinity Capture-MS Homo sapiens
9 PPIP5K1 9677
Affinity Capture-MS Homo sapiens
10 ARPC1B 10095
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 NME7 29922
Affinity Capture-MS Homo sapiens
12 SMG8 55181
Affinity Capture-MS Homo sapiens
13 APEX1 328
Affinity Capture-RNA Homo sapiens
14 C1orf86  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 FBXO7 25793
Affinity Capture-MS Homo sapiens
16 AAAS 8086
Affinity Capture-MS Homo sapiens
17 C11orf73 51501
Affinity Capture-MS Homo sapiens
18 TBC1D2B 23102
Affinity Capture-MS Homo sapiens
19 ANKS1A 23294
Affinity Capture-MS Homo sapiens
20 DDX58 23586
Affinity Capture-RNA Homo sapiens
21 NCDN 23154
Affinity Capture-MS Homo sapiens
22 VBP1 7411
Affinity Capture-MS Homo sapiens
23 VPS33A 65082
Affinity Capture-MS Homo sapiens
24 ARHGEF1 9138
Affinity Capture-MS Homo sapiens
25 ACTR8 93973
Affinity Capture-MS Homo sapiens
26 NCAPD2 9918
Affinity Capture-MS Homo sapiens
27 SIRPD  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 NT5C 30833
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
29 RIN1 9610
Affinity Capture-MS Homo sapiens
30 FLNC 2318
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 GTF2H4 2968
Affinity Capture-MS Homo sapiens
32 ZNF581  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 ARAP3 64411
Affinity Capture-MS Homo sapiens
34 VCPIP1 80124
Affinity Capture-MS Homo sapiens
35 RAB23 51715
Affinity Capture-MS Homo sapiens
36 GPAT2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NT5C is involved
PathwayEvidenceSource
Metabolism TAS Reactome
Metabolism of nucleotides TAS Reactome
Nucleotide catabolism TAS Reactome
Purine catabolism TAS Reactome
Pyrimidine catabolism TAS Reactome





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