Gene description for GTF2H4
Gene name general transcription factor IIH, polypeptide 4, 52kDa
Gene symbol GTF2H4
Other names/aliases P52
TFB2
TFIIH
Species Homo sapiens
 Database cross references - GTF2H4
ExoCarta ExoCarta_2968
Vesiclepedia VP_2968
Entrez Gene 2968
HGNC 4658
MIM 601760
UniProt Q92759  
 GTF2H4 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for GTF2H4
Molecular Function
    protein kinase activity GO:0004672 IDA
    protein binding GO:0005515 IPI
    ATP-dependent DNA helicase activity GO:0004003 IEA
    RNA polymerase II carboxy-terminal domain kinase activity GO:0008353 IDA
    DNA-dependent ATPase activity GO:0008094 IDA
    sequence-specific DNA binding transcription factor activity GO:0003700 TAS
Biological Process
    DNA repair GO:0006281 TAS
    transcription initiation from RNA polymerase I promoter GO:0006361 TAS
    regulation of transcription, DNA-templated GO:0006355 IEA
    protein phosphorylation GO:0006468 IDA
    viral process GO:0016032 TAS
    transcription from RNA polymerase I promoter GO:0006360 TAS
    positive regulation of viral transcription GO:0050434 TAS
    DNA duplex unwinding GO:0032508 IEA
    7-methylguanosine mRNA capping GO:0006370 TAS
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    negative regulation of gene expression, epigenetic GO:0045814 TAS
    transcription elongation from RNA polymerase II promoter GO:0006368 TAS
    gene expression GO:0010467 TAS
    nucleotide-excision repair, DNA damage removal GO:0000718 TAS
    transcription from RNA polymerase II promoter GO:0006366 TAS
    transcription elongation from RNA polymerase I promoter GO:0006362 TAS
    regulation of gene expression, epigenetic GO:0040029 TAS
    termination of RNA polymerase I transcription GO:0006363 TAS
    transcription-coupled nucleotide-excision repair GO:0006283 TAS
    nucleotide-excision repair GO:0006289 TAS
Subcellular Localization
    core TFIIH complex GO:0000439 IEA
    holo TFIIH complex GO:0005675 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified GTF2H4 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GTF2H4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GTF2E1 2960
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
Invitro Homo sapiens
2 BTAF1 9044
Co-purification Homo sapiens
3 TBP  
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
4 GTF2F1 2962
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
5 ERCC3  
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
6 SURB7  
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
7 BRCA1 672
Co-purification Homo sapiens
8 GTF2H5  
Invitro Homo sapiens
9 POLR2A 5430
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
10 GTF2B 2959
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which GTF2H4 is involved
PathwayEvidenceSource
Dual incision reaction in GG-NER TAS Reactome
Dual incision reaction in TC-NER TAS Reactome
Formation of HIV elongation complex in the absence of HIV Tat IEA Reactome
Formation of HIV-1 elongation complex containing HIV-1 Tat IEA Reactome
Formation of incision complex in GG-NER TAS Reactome
Formation of RNA Pol II elongation complex TAS Reactome
Formation of the Early Elongation Complex TAS Reactome
Formation of the HIV-1 Early Elongation Complex IEA Reactome
Formation of transcription-coupled NER (TC-NER) repair complex TAS Reactome
HIV Transcription Initiation IEA Reactome
mRNA Capping TAS Reactome
NoRC negatively regulates rRNA expression IEA Reactome
RNA Pol II CTD phosphorylation and interaction with CE TAS Reactome
RNA Pol II CTD phosphorylation and interaction with CE IEA Reactome
RNA Polymerase I Chain Elongation TAS Reactome
RNA Polymerase I Promoter Escape TAS Reactome
RNA Polymerase I Transcription Initiation TAS Reactome
RNA Polymerase I Transcription Termination TAS Reactome
RNA Polymerase II HIV Promoter Escape IEA Reactome
RNA Polymerase II Pre-transcription Events TAS Reactome
RNA Polymerase II Promoter Escape TAS Reactome
RNA Polymerase II Transcription Elongation TAS Reactome
RNA Polymerase II Transcription Initiation TAS Reactome
RNA Polymerase II Transcription Initiation And Promoter Clearance TAS Reactome
RNA Polymerase II Transcription Pre-Initiation And Promoter Opening TAS Reactome
Tat-mediated elongation of the HIV-1 transcript TAS Reactome
Transcription of the HIV genome IEA Reactome





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