Gene description for GTF2H1
Gene name general transcription factor IIH, polypeptide 1, 62kDa
Gene symbol GTF2H1
Other names/aliases BTF2
P62
TFB1
TFIIH
Species Homo sapiens
 Database cross references - GTF2H1
ExoCarta ExoCarta_2965
Vesiclepedia VP_2965
Entrez Gene 2965
HGNC 4655
MIM 189972
UniProt P32780  
 GTF2H1 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for GTF2H1
Molecular Function
    protein kinase activity GO:0004672 IDA
    DNA-dependent ATPase activity GO:0008094 IDA
    protein binding GO:0005515 IPI
    RNA polymerase II carboxy-terminal domain kinase activity GO:0008353 IDA
Biological Process
    gene expression GO:0010467 TAS
    regulation of gene expression, epigenetic GO:0040029 TAS
    termination of RNA polymerase I transcription GO:0006363 TAS
    positive regulation of viral transcription GO:0050434 TAS
    7-methylguanosine mRNA capping GO:0006370 TAS
    transcription from RNA polymerase I promoter GO:0006360 TAS
    regulation of cyclin-dependent protein serine/threonine kinase activity GO:0000079 TAS
    viral process GO:0016032 TAS
    transcription-coupled nucleotide-excision repair GO:0006283 TAS
    DNA repair GO:0006281 TAS
    transcription elongation from RNA polymerase II promoter GO:0006368 TAS
    protein phosphorylation GO:0006468 IDA
    nucleotide-excision repair GO:0006289 TAS
    transcription elongation from RNA polymerase I promoter GO:0006362 TAS
    transcription from RNA polymerase II promoter GO:0006366 TAS
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
    transcription initiation from RNA polymerase I promoter GO:0006361 TAS
    negative regulation of gene expression, epigenetic GO:0045814 TAS
    nucleotide-excision repair, DNA damage removal GO:0000718 TAS
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
Subcellular Localization
    holo TFIIH complex GO:0005675 IDA
    nucleoplasm GO:0005654 TAS
    core TFIIH complex GO:0000439 IEA
 Experiment description of studies that identified GTF2H1 in exosomes
1
Experiment ID 235
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for GTF2H1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GTF2E1 2960
Invitro Homo sapiens
2 ERCC5  
Invivo Homo sapiens
Invitro Homo sapiens
3 TCEA1 6917
Reconstituted Complex Homo sapiens
4 E2F1 1869
Invivo Homo sapiens
Invitro Homo sapiens
5 CCNH  
Invitro Homo sapiens
6 ERCC3  
Invivo Homo sapiens
Invitro Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
7 AR 367
Invivo Homo sapiens
Invitro Homo sapiens
8 CCSER2 54462
Two-hybrid Homo sapiens
9 PSMC2 5701
Invivo Homo sapiens
Invitro Homo sapiens
10 FUBP1 8880
Invivo Homo sapiens
Invitro Homo sapiens
11 CDK7 1022
Affinity Capture-Western Homo sapiens
12 HNRNPU 3192
Invivo Homo sapiens
13 TP53 7157
Reconstituted Complex Homo sapiens
14 ESR1  
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
15 ERCC2 2068
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
16 MCM2 4171
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which GTF2H1 is involved
PathwayEvidenceSource
Dual incision reaction in GG-NER TAS Reactome
Dual incision reaction in TC-NER TAS Reactome
Formation of HIV elongation complex in the absence of HIV Tat IEA Reactome
Formation of HIV-1 elongation complex containing HIV-1 Tat IEA Reactome
Formation of incision complex in GG-NER TAS Reactome
Formation of RNA Pol II elongation complex TAS Reactome
Formation of the Early Elongation Complex TAS Reactome
Formation of the HIV-1 Early Elongation Complex IEA Reactome
Formation of transcription-coupled NER (TC-NER) repair complex TAS Reactome
HIV Transcription Initiation IEA Reactome
mRNA Capping TAS Reactome
NoRC negatively regulates rRNA expression IEA Reactome
RNA Pol II CTD phosphorylation and interaction with CE TAS Reactome
RNA Pol II CTD phosphorylation and interaction with CE IEA Reactome
RNA Polymerase I Chain Elongation TAS Reactome
RNA Polymerase I Promoter Escape TAS Reactome
RNA Polymerase I Transcription Initiation TAS Reactome
RNA Polymerase I Transcription Termination TAS Reactome
RNA Polymerase II HIV Promoter Escape IEA Reactome
RNA Polymerase II Pre-transcription Events TAS Reactome
RNA Polymerase II Promoter Escape TAS Reactome
RNA Polymerase II Transcription Elongation TAS Reactome
RNA Polymerase II Transcription Initiation TAS Reactome
RNA Polymerase II Transcription Initiation And Promoter Clearance TAS Reactome
RNA Polymerase II Transcription Pre-Initiation And Promoter Opening TAS Reactome
Tat-mediated elongation of the HIV-1 transcript TAS Reactome
Transcription of the HIV genome IEA Reactome





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