Gene description for TRIOBP
Gene name TRIO and F-actin binding protein
Gene symbol TRIOBP
Other names/aliases DFNB28
HRIHFB2122
TAP68
TARA
dJ37E16.4
Species Homo sapiens
 Database cross references - TRIOBP
ExoCarta ExoCarta_11078
Vesiclepedia VP_11078
Entrez Gene 11078
HGNC 17009
MIM 609761
UniProt Q9H2D6  
 TRIOBP identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for TRIOBP
Molecular Function
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 NAS
    ubiquitin protein ligase binding GO:0031625 IPI
    myosin II binding GO:0045159 NAS
    actin filament binding GO:0051015 IBA
    actin filament binding GO:0051015 IDA
Biological Process
    sensory perception of sound GO:0007605 IEA
    actin modification GO:0030047 NAS
    barbed-end actin filament capping GO:0051016 NAS
    cell division GO:0051301 IEA
    auditory receptor cell stereocilium organization GO:0060088 IEA
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 IBA
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 IDA
Subcellular Localization
    chromosome, telomeric region GO:0000781 IEA
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IEA
    centrosome GO:0005813 IEA
    focal adhesion GO:0005925 HDA
    actin cytoskeleton GO:0015629 IBA
    actin cytoskeleton GO:0015629 IDA
    actin cytoskeleton GO:0015629 NAS
    midbody GO:0030496 IEA
    stereocilium base GO:0120044 IEA
 Experiment description of studies that identified TRIOBP in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for TRIOBP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATG16L1 55054
Affinity Capture-MS Homo sapiens
2 PCM1 5108
Proximity Label-MS Homo sapiens
3 ANLN 54443
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 MCMBP 79892
Affinity Capture-MS Homo sapiens
5 MECP2 4204
Affinity Capture-MS Homo sapiens
6 KIF2A 3796
Affinity Capture-MS Homo sapiens
7 THAP8  
Affinity Capture-MS Homo sapiens
8 PTOV1  
Affinity Capture-MS Homo sapiens
9 EIF3K 27335
Affinity Capture-MS Homo sapiens
10 PPP1CB 5500
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
11 POLR2F  
Affinity Capture-MS Homo sapiens
12 KIF14 9928
Affinity Capture-MS Homo sapiens
13 OCLM  
Affinity Capture-MS Homo sapiens
14 Myh9 17886
Affinity Capture-MS Mus musculus
15 WDR77 79084
Affinity Capture-MS Homo sapiens
16 GSK3B 2932
Affinity Capture-Western Homo sapiens
17 VAPA 9218
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 JPH4 84502
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 RABEPK  
Affinity Capture-MS Homo sapiens
20 C15orf59  
Affinity Capture-MS Homo sapiens
21 VTN 7448
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 VIM 7431
Two-hybrid Homo sapiens
23 FXR1 8087
Affinity Capture-MS Homo sapiens
24 POLR1C 9533
Two-hybrid Homo sapiens
25 RLIM 51132
Affinity Capture-MS Homo sapiens
26 ALDH1A3 220
Affinity Capture-MS Homo sapiens
27 HAUS2  
Affinity Capture-MS Homo sapiens
28 EPB41L5 57669
Affinity Capture-MS Homo sapiens
29 MYO18A 399687
Affinity Capture-MS Homo sapiens
30 IKBKG 8517
Two-hybrid Homo sapiens
31 PACSIN2 11252
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 ERLEC1 27248
Affinity Capture-MS Homo sapiens
33 CAPZA2 830
Affinity Capture-MS Homo sapiens
34 MCC 4163
Affinity Capture-MS Homo sapiens
35 KIAA0368 23392
Affinity Capture-MS Homo sapiens
36 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
37 GAGE5  
Affinity Capture-MS Homo sapiens
38 RDH13  
Affinity Capture-MS Homo sapiens
39 PARK2  
Affinity Capture-MS Homo sapiens
40 PACSIN3 29763
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 Actb 11461
Affinity Capture-MS Mus musculus
42 RAI14 26064
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
43 CORO6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 RILP 83547
Affinity Capture-MS Homo sapiens
45 PACSIN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 MYO1C 4641
Affinity Capture-MS Homo sapiens
47 SKAP2 8935
Affinity Capture-MS Homo sapiens
48 BAG6 7917
Affinity Capture-MS Homo sapiens
49 KLHDC2  
Affinity Capture-MS Homo sapiens
50 HECTD3 79654
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
51 DNAJC5 80331
Proximity Label-MS Homo sapiens
52 PPP1R12A 4659
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
53 TTC19  
Affinity Capture-MS Homo sapiens
54 TMEM257  
Affinity Capture-MS Homo sapiens
55 TMEM132A 54972
Affinity Capture-MS Homo sapiens
56 YAP1 10413
Affinity Capture-MS Homo sapiens
57 MYH9 4627
Affinity Capture-MS Homo sapiens
58 DYRK1A 1859
Affinity Capture-MS Homo sapiens
59 VAPB 9217
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 KXD1 79036
Affinity Capture-MS Homo sapiens
61 SORT1 6272
Affinity Capture-MS Homo sapiens
62 WHAMMP3  
Affinity Capture-MS Homo sapiens
63 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
64 PLK1 5347
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
65 EPHA2 1969
Proximity Label-MS Homo sapiens
66 DYRK2 8445
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
67 IQGAP1 8826
Affinity Capture-MS Homo sapiens
68 NDEL1 81565
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
69 CIZ1  
Affinity Capture-MS Homo sapiens
70 ENTHD1  
Affinity Capture-MS Homo sapiens
71 FYTTD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 Pparg 19016
Affinity Capture-MS Mus musculus
73 SRXN1 140809
Affinity Capture-MS Homo sapiens
74 GTF2H1 2965
Two-hybrid Homo sapiens
75 MPRIP 23164
Affinity Capture-MS Homo sapiens
76 UACA 55075
Affinity Capture-MS Homo sapiens
77 L1TD1  
Affinity Capture-MS Homo sapiens
78 Ppp1cb 19046
Affinity Capture-MS Mus musculus
79 KIAA1328  
Affinity Capture-MS Homo sapiens
80 Plk1  
Affinity Capture-MS Mus musculus
81 C17orf59 54785
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 SOX2  
Affinity Capture-MS Homo sapiens
83 TNNT1  
Affinity Capture-MS Homo sapiens
84 PSMC3 5702
Affinity Capture-MS Homo sapiens
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