Gene description for SKAP2
Gene name src kinase associated phosphoprotein 2
Gene symbol SKAP2
Other names/aliases PRAP
RA70
SAPS
SCAP2
SKAP-HOM
SKAP55R
Species Homo sapiens
 Database cross references - SKAP2
ExoCarta ExoCarta_8935
Vesiclepedia VP_8935
Entrez Gene 8935
HGNC 15687
MIM 605215
UniProt O75563  
 SKAP2 identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for SKAP2
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    signal transduction GO:0007165 TAS
    negative regulation of cell population proliferation GO:0008285 IEA
    B cell activation GO:0042113 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified SKAP2 in exosomes
1
Experiment ID 231
MISEV standards
Biophysical techniques
Alix|CD63|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SKAP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LYN 4067
Affinity Capture-Western Homo sapiens
2 RAP1GAP 5909
Affinity Capture-MS Homo sapiens
3 ZNF174  
Affinity Capture-MS Homo sapiens
4 ATP13A3 79572
Affinity Capture-MS Homo sapiens
5 GRB2 2885
Affinity Capture-MS Homo sapiens
6 FBXO7 25793
Affinity Capture-MS Homo sapiens
7 GBA2  
Affinity Capture-MS Homo sapiens
8 SNRPB 6628
Affinity Capture-MS Homo sapiens
9 LINC01588  
Affinity Capture-MS Homo sapiens
10 C1orf168  
Affinity Capture-MS Homo sapiens
11 IRS4 8471
Affinity Capture-MS Homo sapiens
12 FASLG 356
Protein-peptide Homo sapiens
13 HIST1H2BN 8341
Cross-Linking-MS (XL-MS) Homo sapiens
14 ERCC4  
Affinity Capture-MS Homo sapiens
15 LOC102724334 102724334
Cross-Linking-MS (XL-MS) Homo sapiens
16 HCK 3055
Affinity Capture-Western Homo sapiens
17 APP 351
Reconstituted Complex Homo sapiens
18 PRAM1  
Affinity Capture-Western Homo sapiens
19 HIST1H2BO 8348
Cross-Linking-MS (XL-MS) Homo sapiens
20 FYB 2533
Affinity Capture-MS Homo sapiens
21 PRPS2 5634
Affinity Capture-MS Homo sapiens
22 SKAP1 8631
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 SPATA5L1 79029
Affinity Capture-MS Homo sapiens
24 FRMD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 RGS3 5998
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 FYN 2534
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
27 PTK2B 2185
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
28 TRIOBP 11078
Affinity Capture-MS Homo sapiens
29 SNRPN 6638
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 SRC 6714
Biochemical Activity Homo sapiens
31 C9orf72  
Affinity Capture-MS Homo sapiens
32 KHDRBS1 10657
Protein-peptide Homo sapiens
33 SNTB2 6645
Affinity Capture-MS Homo sapiens
34 ABHD5 51099
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 TMPRSS13  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SKAP2 is involved
PathwayEvidenceSource
Cell-Cell communication TAS Reactome
Signal regulatory protein family interactions TAS Reactome





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