Gene description for SKAP2
Gene name src kinase associated phosphoprotein 2
Gene symbol SKAP2
Other names/aliases PRAP
RA70
SAPS
SCAP2
SKAP-HOM
SKAP55R
Species Homo sapiens
 Database cross references - SKAP2
ExoCarta ExoCarta_8935
Entrez Gene 8935
HGNC 15687
MIM 605215
UniProt O75563  
 SKAP2 identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for SKAP2
Molecular Function
    SH3/SH2 adaptor activity GO:0005070 TAS
    protein binding GO:0005515 IPI
Biological Process
    protein complex assembly GO:0006461 TAS
    positive regulation of signal transduction GO:0009967 TAS
    signal transduction GO:0007165 TAS
    B cell activation GO:0042113 IEA
    negative regulation of cell proliferation GO:0008285 IEA
Subcellular Localization
    plasma membrane GO:0005886 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    nucleoplasm GO:0005654 IDA
 Experiment description of studies that identified SKAP2 in exosomes
1
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SKAP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HCK 3055
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
2 LYN 4067
Affinity Capture-Western Homo sapiens
3 FYN 2534
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
4 PRAM-1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which SKAP2 is involved
PathwayEvidenceSource
Signal regulatory protein (SIRP) family interactions TAS Reactome





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