Gene description for RAP1GAP
Gene name RAP1 GTPase activating protein
Gene symbol RAP1GAP
Other names/aliases RAP1GA1
RAP1GAP1
RAP1GAPII
RAPGAP
Species Homo sapiens
 Database cross references - RAP1GAP
ExoCarta ExoCarta_5909
Entrez Gene 5909
HGNC 9858
MIM 600278
UniProt P47736  
 RAP1GAP identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for RAP1GAP
Molecular Function
    GTPase activity GO:0003924 NAS
    protein homodimerization activity GO:0042803 IPI
    Ras GTPase binding GO:0017016 IPI
    GTPase activator activity GO:0005096 IDA
    protein binding GO:0005515 IPI
Biological Process
    metabolic process GO:0008152 NAS
    regulation of GTPase activity GO:0043087 IDA
    signal transduction GO:0007165 NAS
    negative regulation of microvillus assembly GO:1903697 IMP
    positive regulation of GTPase activity GO:0043547 IDA
Subcellular Localization
    cytosol GO:0005829 TAS
    Golgi membrane GO:0000139 IEA
    membrane GO:0016020 IDA
 Experiment description of studies that identified RAP1GAP in exosomes
1
Experiment ID 236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for RAP1GAP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GNAZ 2781
Invitro Homo sapiens
2 PFN2 5217
Two-hybrid Homo sapiens
3 BRAF  
Reconstituted Complex Homo sapiens
4 MEX3B 84206
Two-hybrid Homo sapiens
5 MLLT4 4301
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
6 RAP1A 5906
Biochemical Activity Homo sapiens
View the network image/svg+xml
 Pathways in which RAP1GAP is involved
PathwayEvidenceSource
Rap1 signalling TAS Reactome





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