Gene description for Ppp1cb
Gene name protein phosphatase 1, catalytic subunit, beta isoform
Gene symbol Ppp1cb
Other names/aliases 1200010B19
Species Mus musculus
 Database cross references - Ppp1cb
ExoCarta ExoCarta_19046
Vesiclepedia VP_19046
Entrez Gene 19046
UniProt P62141  
 Ppp1cb identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ppp1cb
Molecular Function
    phosphoprotein phosphatase activity GO:0004721 ISO
    protein serine/threonine phosphatase activity GO:0004722 IBA
    protein serine/threonine phosphatase activity GO:0004722 IDA
    protein binding GO:0005515 IPI
    phosphatase activity GO:0016791 IDA
    myosin phosphatase activity GO:0017018 ISS
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    calmodulin-dependent protein phosphatase activity GO:0033192 IEA
    metal ion binding GO:0046872 IEA
    myosin-light-chain-phosphatase activity GO:0050115 ISO
    myosin-light-chain-phosphatase activity GO:0050115 ISS
    histone H2AXS140 phosphatase activity GO:0140791 IEA
    RNA polymerase II CTD heptapeptide repeat Y1 phosphatase activity GO:0180004 IEA
    RNA polymerase II CTD heptapeptide repeat T4 phosphatase activity GO:0180005 IEA
    RNA polymerase II CTD heptapeptide repeat S2 phosphatase activity GO:0180006 IEA
    RNA polymerase II CTD heptapeptide repeat S5 phosphatase activity GO:0180007 IEA
    RNA polymerase II CTD heptapeptide repeat S7 phosphatase activity GO:0180008 IEA
    MAP kinase serine/threonine phosphatase activity GO:1990439 IEA
Biological Process
    glycogen metabolic process GO:0005977 IEA
    regulation of glycogen biosynthetic process GO:0005979 ISO
    regulation of glycogen catabolic process GO:0005981 ISO
    chromatin remodeling GO:0006338 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    protein dephosphorylation GO:0006470 IDA
    protein dephosphorylation GO:0006470 IDA
    regulation of cell adhesion GO:0030155 ISO
    regulation of cell adhesion GO:0030155 ISS
    circadian regulation of gene expression GO:0032922 IBA
    circadian regulation of gene expression GO:0032922 IDA
    regulation of circadian rhythm GO:0042752 IBA
    regulation of circadian rhythm GO:0042752 IDA
    regulation of circadian rhythm GO:0042752 IMP
    regulation of circadian rhythm GO:0042752 ISO
    entrainment of circadian clock by photoperiod GO:0043153 IMP
    cell division GO:0051301 IEA
Subcellular Localization
    protein phosphatase type 1 complex GO:0000164 ISO
    chromosome, telomeric region GO:0000781 IEA
    chromosome, telomeric region GO:0000781 ISO
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 IEA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    glycogen granule GO:0042587 ISO
    PTW/PP1 phosphatase complex GO:0072357 ISO
    PTW/PP1 phosphatase complex GO:0072357 ISS
 Experiment description of studies that identified Ppp1cb in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ppp1cb
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Unk  
Affinity Capture-RNA Mus musculus
2 AAK1 22848
Affinity Capture-MS Homo sapiens
3 SMURF1 57154
Affinity Capture-Luminescence Homo sapiens
4 PARD3 56288
Affinity Capture-MS Homo sapiens
5 DBN1 1627
Affinity Capture-MS Homo sapiens
6 Cebpa  
Affinity Capture-MS Mus musculus
7 CLTB 1212
Affinity Capture-MS Homo sapiens
8 ANTXR1 84168
Affinity Capture-MS Homo sapiens
9 Hspa1b 15511
Affinity Capture-Western Mus musculus
10 PPP1R12A 4659
Affinity Capture-MS Homo sapiens
11 SPECC1L 23384
Affinity Capture-MS Homo sapiens
12 AP2A1 160
Affinity Capture-MS Homo sapiens
13 ZNF638 27332
Affinity Capture-MS Homo sapiens
14 TRIOBP 11078
Affinity Capture-MS Homo sapiens
15 YES1 7525
Affinity Capture-MS Homo sapiens
16 SCRIB 23513
Affinity Capture-MS Homo sapiens
17 Atg16l1  
Affinity Capture-MS Mus musculus
18 MYO1B 4430
Affinity Capture-MS Homo sapiens
19 Eed  
Affinity Capture-MS Mus musculus
20 PPP1R15B  
Affinity Capture-MS Homo sapiens
21 Wdr82  
Co-fractionation Mus musculus
22 TWF1 5756
Affinity Capture-MS Homo sapiens
23 PABPC4 8761
Affinity Capture-MS Homo sapiens
24 COBL  
Affinity Capture-MS Homo sapiens
25 PPP1CA 5499
Affinity Capture-MS Homo sapiens
26 MYO18A 399687
Affinity Capture-MS Homo sapiens
27 Dlg4  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
28 SORBS2  
Affinity Capture-MS Homo sapiens
29 LUZP1 7798
Affinity Capture-MS Homo sapiens
30 RBM6 10180
Affinity Capture-MS Homo sapiens
31 RAI14 26064
Affinity Capture-MS Homo sapiens
32 TMOD1  
Affinity Capture-MS Homo sapiens
33 PPP1R16A 84988
Affinity Capture-MS Homo sapiens
34 HTT 3064
Affinity Capture-MS Homo sapiens
35 LRRFIP2 9209
Affinity Capture-MS Homo sapiens
36 Agap2  
Affinity Capture-MS Mus musculus
37 PPP1R8 5511
Affinity Capture-MS Homo sapiens
38 TOX4  
Affinity Capture-MS Homo sapiens
39 BMP2K 55589
Affinity Capture-MS Homo sapiens
40 DHX9 1660
Affinity Capture-MS Homo sapiens
41 Ppp1r8  
Co-fractionation Mus musculus
42 PPP1R12C 54776
Affinity Capture-MS Homo sapiens
43 KIF18A  
Affinity Capture-MS Homo sapiens
44 PPP1R12B 4660
Affinity Capture-MS Homo sapiens
45 STON2 85439
Affinity Capture-MS Homo sapiens
46 Mycbp2 105689
Affinity Capture-MS Mus musculus
47 AHNAK 79026
Affinity Capture-MS Homo sapiens
48 YLPM1 56252
Affinity Capture-MS Homo sapiens
49 SPECC1 92521
Affinity Capture-MS Homo sapiens
50 MYO5A 4644
Affinity Capture-MS Homo sapiens
51 PACSIN3 29763
Affinity Capture-MS Homo sapiens
52 CD59 966
Affinity Capture-MS Homo sapiens
53 UACA 55075
Affinity Capture-MS Homo sapiens
54 MYO1C 4641
Affinity Capture-MS Homo sapiens
55 Max  
Affinity Capture-Western Mus musculus
56 AP2M1 1173
Affinity Capture-MS Homo sapiens
57 SYNPO 11346
Affinity Capture-MS Homo sapiens
58 Pou5f1  
Affinity Capture-MS Mus musculus
59 TMOD3 29766
Affinity Capture-MS Homo sapiens
60 PPP1R10  
Affinity Capture-MS Homo sapiens
61 FLII 2314
Affinity Capture-MS Homo sapiens
62 CLTC 1213
Affinity Capture-MS Homo sapiens
63 MPRIP 23164
Affinity Capture-MS Homo sapiens
64 Tia1  
Affinity Capture-MS Mus musculus
65 Ppp1cc 19047
Co-fractionation Mus musculus
66 GSN 2934
Affinity Capture-MS Homo sapiens
67 SVIL 6840
Affinity Capture-MS Homo sapiens
68 SPTBN1 6711
Affinity Capture-MS Homo sapiens
69 SPTAN1 6709
Affinity Capture-MS Homo sapiens
70 Ccnb1  
Affinity Capture-Western Mus musculus
71 DAB2 1601
Affinity Capture-MS Homo sapiens
72 JUP 3728
Affinity Capture-MS Homo sapiens
73 SSFA2 6744
Affinity Capture-MS Homo sapiens
74 PPP1R13L 10848
Affinity Capture-MS Homo sapiens
75 MISP 126353
Affinity Capture-MS Homo sapiens
76 RIF1  
Affinity Capture-MS Homo sapiens
77 Kctd13  
Affinity Capture-MS Mus musculus
78 CAPZA2 830
Affinity Capture-MS Homo sapiens
79 Ccnd3  
Affinity Capture-Western Mus musculus
80 AFAP1 60312
Affinity Capture-MS Homo sapiens
81 PPP1R2 5504
Affinity Capture-MS Homo sapiens
82 KIF18B 146909
Affinity Capture-MS Homo sapiens
83 CLTA 1211
Affinity Capture-MS Homo sapiens
84 MYO1E 4643
Affinity Capture-MS Homo sapiens
85 WDR92 116143
Affinity Capture-MS Homo sapiens
86 SH2D4A 63898
Affinity Capture-MS Homo sapiens
87 ABLIM1 3983
Affinity Capture-MS Homo sapiens
88 DSG2 1829
Affinity Capture-MS Homo sapiens
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