Experiment description of studies that identified PPP1R16A in exosomes

1 Experiment ID 363 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 364 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 365 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

5 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for PPP1R16A

Protein Interactor ExoCarta ID Identification method PubMed Species 1 KRTAP19-5   Two-hybrid Homo sapiens 2 ATXN7L2   Two-hybrid Homo sapiens 3 TRIP6 7205 Two-hybrid Homo sapiens 4 ZNF414   Two-hybrid Homo sapiens 5 MRPL12 6182 Two-hybrid Homo sapiens 6 IDO1   Two-hybrid Homo sapiens 7 NUTF2 10204 Two-hybrid Homo sapiens 8 RABGGTA 5875 Affinity Capture-MS Homo sapiens 9 BATF2   Two-hybrid Homo sapiens 10 C1orf216   Two-hybrid Homo sapiens 11 NEK6 10783 Two-hybrid Homo sapiens 12 IL16   Two-hybrid Homo sapiens 13 AES 166 Two-hybrid Homo sapiens 14 Iqgap3 404710 Affinity Capture-MS Mus musculus 15 C3orf36   Two-hybrid Homo sapiens 16 CDCA7L   Two-hybrid Homo sapiens 17 PPP1CA 5499 Two-hybrid Homo sapiens 18 PHPT1 29085 Two-hybrid Homo sapiens 19 PBDC1 51260 Two-hybrid Homo sapiens 20 CDH24   Two-hybrid Homo sapiens 21 AVPI1   Two-hybrid Homo sapiens 22 TXNRD1 7296 Two-hybrid Homo sapiens 23 FSD1   Two-hybrid Homo sapiens 24 MYOM1   Two-hybrid Homo sapiens 25 TTC23   Two-hybrid Homo sapiens 26 PUF60 22827 Two-hybrid Homo sapiens Reconstituted Complex Homo sapiens 27 RABGGTB 5876 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 28 DDX58 23586 Affinity Capture-RNA Homo sapiens 29 XRCC6BP1   Two-hybrid Homo sapiens 30 PPP1R16A 84988 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 31 PPP1CC 5501 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 32 LZTS2 84445 Two-hybrid Homo sapiens 33 RGMA   Two-hybrid Homo sapiens 34 CCDC116   Two-hybrid Homo sapiens 35 FAM173A   Two-hybrid Homo sapiens 36 TSSK3   Two-hybrid Homo sapiens 37 PPP1CB 5500 Two-hybrid Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens 38 SCNM1   Two-hybrid Homo sapiens 39 PAPSS1 9061 Two-hybrid Homo sapiens 40 CKMT1A 548596 Two-hybrid Homo sapiens 41 MCCD1   Two-hybrid Homo sapiens 42 KAT5   Two-hybrid Homo sapiens 43 HGS 9146 Reconstituted Complex Homo sapiens 44 TPP2 7174 Two-hybrid Homo sapiens 45 SUV39H1   Two-hybrid Homo sapiens 46 PRKAB2 5565 Two-hybrid Homo sapiens 47 TFIP11   Two-hybrid Homo sapiens 48 Ppp1cb 19046 Affinity Capture-MS Mus musculus 49 PRPF31 26121 Two-hybrid Homo sapiens 50 DDX27 55661 Two-hybrid Homo sapiens 51 CKMT1B 1159 Two-hybrid Homo sapiens View the network

Pathways in which PPP1R16A is involved

No pathways found