Gene description for MRPL12
Gene name mitochondrial ribosomal protein L12
Gene symbol MRPL12
Other names/aliases 5c5-2
L12mt
MRP-L31/34
MRPL7
MRPL7/L12
RPML12
Species Homo sapiens
 Database cross references - MRPL12
ExoCarta ExoCarta_6182
Vesiclepedia VP_6182
Entrez Gene 6182
HGNC 10378
MIM 602375
UniProt P52815  
 MRPL12 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for MRPL12
Molecular Function
    RNA binding GO:0003723 TAS
    mRNA binding GO:0003729 IBA
    structural constituent of ribosome GO:0003735 IBA
    protein binding GO:0005515 IPI
Biological Process
    mitochondrial transcription GO:0006390 IDA
    translation GO:0006412 IBA
    mitochondrial translation GO:0032543 NAS
    positive regulation of DNA-templated transcription GO:0045893 IDA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 NAS
    mitochondrial inner membrane GO:0005743 TAS
    mitochondrial matrix GO:0005759 IDA
    mitochondrial matrix GO:0005759 TAS
    mitochondrial large ribosomal subunit GO:0005762 IBA
    mitochondrial large ribosomal subunit GO:0005762 IDA
    mitochondrial large ribosomal subunit GO:0005762 NAS
 Experiment description of studies that identified MRPL12 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MRPL12
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SNRPB2 6629
Affinity Capture-MS Homo sapiens
2 MINOS1-NBL1  
Two-hybrid Homo sapiens
3 MRPS27 23107
Affinity Capture-Western Homo sapiens
4 PPP6R2 9701
Affinity Capture-MS Homo sapiens
5 UBE2H 7328
Affinity Capture-MS Homo sapiens
6 SLIRP 81892
Proximity Label-MS Homo sapiens
7 TFB1M  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 MRPL48  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 HDAC2 3066
Affinity Capture-MS Homo sapiens
10 BCKDK 10295
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 GTPBP8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 NME6  
Affinity Capture-MS Homo sapiens
13 COX15 1355
Proximity Label-MS Homo sapiens
14 MRPL33  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 RIT1 6016
Negative Genetic Homo sapiens
16 TARDBP 23435
Affinity Capture-MS Homo sapiens
17 ADRB2  
Affinity Capture-MS Homo sapiens
18 Ybx1 22608
Affinity Capture-MS Mus musculus
19 ACTC1 70
Affinity Capture-MS Homo sapiens
20 CD2AP 23607
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 MTG2  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 NGRN  
Proximity Label-MS Homo sapiens
23 SEPT5 5413
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 CHCHD1  
Proximity Label-MS Homo sapiens
25 MRPL10 124995
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 MRPL27 51264
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 RPL14 9045
Co-fractionation Homo sapiens
28 THOC7 80145
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 MRPL50 54534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 HARS2 23438
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 CAND1 55832
Affinity Capture-MS Homo sapiens
32 MRPL52  
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 MRRF  
Proximity Label-MS Homo sapiens
34 MRPL49 740
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 MRPL44  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 DNAJC15  
Proximity Label-MS Homo sapiens
37 MTRF1  
Proximity Label-MS Homo sapiens
38 GFM2 84340
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 CORO1A 11151
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 CCDC90B  
Proximity Label-MS Homo sapiens
41 RPL26L1 51121
Affinity Capture-MS Homo sapiens
42 SS18L2  
Affinity Capture-MS Homo sapiens
43 LRPPRC 10128
Proximity Label-MS Homo sapiens
44 SEPT9 10801
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 TRUB2  
Proximity Label-MS Homo sapiens
46 DDX23 9416
Cross-Linking-MS (XL-MS) Homo sapiens
47 MRPL22  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 RDX 5962
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 MCU 90550
Proximity Label-MS Homo sapiens
50 YARS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 TFAM 7019
Proximity Label-MS Homo sapiens
52 RBM3 5935
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
53 PMPCA 23203
Proximity Label-MS Homo sapiens
54 HINT2 84681
Proximity Label-MS Homo sapiens
55 TRMT2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 PTPMT1 114971
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 MRPL23 6150
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
58 H2AFV 94239
Cross-Linking-MS (XL-MS) Homo sapiens
59 PRC1 9055
Affinity Capture-MS Homo sapiens
60 C8orf82  
Proximity Label-MS Homo sapiens
61 RPL37A 6168
Cross-Linking-MS (XL-MS) Homo sapiens
62 MRPL12 6182
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
63 SRRM2 23524
Affinity Capture-MS Homo sapiens
64 RPS8 6202
Affinity Capture-MS Homo sapiens
65 MRPL21 219927
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 EIF5B 9669
Affinity Capture-MS Homo sapiens
67 OLIG1  
Two-hybrid Homo sapiens
68 CALM3 808
Affinity Capture-MS Homo sapiens
69 MYCN  
Affinity Capture-MS Homo sapiens
70 MRPS26 64949
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
71 SURF1  
Proximity Label-MS Homo sapiens
72 EFCAB2  
Two-hybrid Homo sapiens
73 SEPT8 23176
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 EXD2  
Proximity Label-MS Homo sapiens
75 MRPL39 54148
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 SEPT7 989
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 CUL1 8454
Affinity Capture-MS Homo sapiens
78 BTRC 8945
Affinity Capture-MS Homo sapiens
79 SEPT1 1731
Two-hybrid Homo sapiens
80 TACO1  
Proximity Label-MS Homo sapiens
81 FASTKD2  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 KLF15  
Affinity Capture-MS Homo sapiens
83 THOC1 9984
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 VWA8 23078
Proximity Label-MS Homo sapiens
85 MRPL47 57129
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 SOD1 6647
Affinity Capture-MS Homo sapiens
87 RPL19 6143
Co-fractionation Homo sapiens
88 MTERF3  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 KIF14 9928
Affinity Capture-MS Homo sapiens
90 GATB  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 TRIM59  
Two-hybrid Homo sapiens
92 PNPT1 87178
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 MDH2 4191
Proximity Label-MS Homo sapiens
94 KLF16  
Affinity Capture-MS Homo sapiens
95 MRPS30 10884
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 CALM2 805
Affinity Capture-MS Homo sapiens
97 MRPL13  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 UBE2N 7334
Affinity Capture-MS Homo sapiens
99 MRPL57  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 C1orf122  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
101 MRPL32 64983
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 SLC25A10 1468
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 C6orf203  
Proximity Label-MS Homo sapiens
104 CMAS 55907
Affinity Capture-MS Homo sapiens
105 MRPL17  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 RC3H2  
Affinity Capture-MS Homo sapiens
107 FASTKD5  
Proximity Label-MS Homo sapiens
108 MRPL45 84311
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
109 PARK2  
Affinity Capture-MS Homo sapiens
110 MALSU1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
111 MRPL20 55052
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 MTFR1L 56181
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 MRPL14 64928
Affinity Capture-MS Homo sapiens
114 POLRMT 5442
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
115 KRTAP10-1  
Two-hybrid Homo sapiens
116 PPP1R16A 84988
Two-hybrid Homo sapiens
117 ARPP19 10776
Cross-Linking-MS (XL-MS) Homo sapiens
118 TUFM 7284
Proximity Label-MS Homo sapiens
119 CBX3 11335
Two-hybrid Homo sapiens
120 HSCB 150274
Proximity Label-MS Homo sapiens
121 WDYHV1  
Two-hybrid Homo sapiens
122 MRPL51 51258
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
123 MRPL46  
Affinity Capture-MS Homo sapiens
124 CCDC109B 55013
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
125 HERC2 8924
Affinity Capture-MS Homo sapiens
126 SMC3 9126
Cross-Linking-MS (XL-MS) Homo sapiens
127 MRPL34  
Affinity Capture-MS Homo sapiens
128 TBRG4 9238
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
129 KIAA0391  
Affinity Capture-MS Homo sapiens
130 MRPL3  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
131 FTSJ2 29960
Affinity Capture-MS Homo sapiens
132 MTFMT  
Proximity Label-MS Homo sapiens
133 MRPL11 65003
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
134 MTRF1L  
Proximity Label-MS Homo sapiens
135 THOC2 57187
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
136 SAT1  
Two-hybrid Homo sapiens
137 TMEM70  
Proximity Label-MS Homo sapiens
138 CYLD  
Affinity Capture-MS Homo sapiens
139 MRPL2 51069
Affinity Capture-MS Homo sapiens
140 SEPT6 23157
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
141 RPL11 6135
Co-fractionation Homo sapiens
142 NEURL4  
Affinity Capture-MS Homo sapiens
143 OXA1L  
Affinity Capture-MS Homo sapiens
144 DST 667
Cross-Linking-MS (XL-MS) Homo sapiens
145 PA2G4 5036
Affinity Capture-MS Homo sapiens
146 HNRNPD 3184
Co-fractionation Homo sapiens
147 MRM1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
148 YBX3 8531
Affinity Capture-MS Homo sapiens
149 OTC 5009
Proximity Label-MS Homo sapiens
150 MRPL40 64976
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
151 AK2 204
Co-fractionation Homo sapiens
152 RPLP2 6181
Co-fractionation Homo sapiens
153 MRPL54  
Affinity Capture-MS Homo sapiens
154 MRPS12  
Proximity Label-MS Homo sapiens
155 RPL23 9349
Co-fractionation Homo sapiens
156 MTIF2 4528
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
157 RPS2 6187
Co-fractionation Homo sapiens
158 PITRM1 10531
Co-fractionation Homo sapiens
159 MRPL24  
Affinity Capture-MS Homo sapiens
160 MRPL35  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
161 CLPP 8192
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
162 MTIF3  
Proximity Label-MS Homo sapiens
163 SEPT3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
164 MSI1  
Affinity Capture-MS Homo sapiens
165 MRPL9 65005
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
166 HIST1H1E 3008
Cross-Linking-MS (XL-MS) Homo sapiens
167 MRPL16  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
168 EPPK1 83481
Co-fractionation Homo sapiens
169 PRKCZ 5590
Affinity Capture-MS Homo sapiens
170 MTG1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
171 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
172 NR3C1 2908
Proximity Label-MS Homo sapiens
173 RMND1 55005
Proximity Label-MS Homo sapiens
174 PDIA3 2923
Affinity Capture-MS Homo sapiens
175 THOC5 8563
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
176 GADD45GIP1  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
177 DHX30 22907
Proximity Label-MS Homo sapiens
178 MORF4L1  
Two-hybrid Homo sapiens
179 PPP6R3 55291
Affinity Capture-MS Homo sapiens
180 SEL1L 6400
Affinity Capture-MS Homo sapiens
181 TXNDC9 10190
Two-hybrid Homo sapiens
182 C17orf80 55028
Proximity Label-MS Homo sapiens
183 MRPL15 29088
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
184 ECI2 10455
Affinity Capture-MS Homo sapiens
185 MRPL1  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
186 PMPCB 9512
Proximity Label-MS Homo sapiens
187 MAP7D1 55700
Affinity Capture-MS Homo sapiens
188 SEPT2 4735
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
189 SOAT1 6646
Affinity Capture-MS Homo sapiens
190 MRPL4 51073
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
191 MRPL30  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
192 RPUSD4 84881
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
193 MYC  
Affinity Capture-MS Homo sapiens
194 FOXP2 93986
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
195 SDR39U1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
196 MRPL37 51253
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
197 DLST 1743
Affinity Capture-MS Homo sapiens
198 TSFM 10102
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
199 RPL12 6136