Gene description for HERC2
Gene name HECT and RLD domain containing E3 ubiquitin protein ligase 2
Gene symbol HERC2
Other names/aliases D15F37S1
MRT38
SHEP1
jdf2
p528
Species Homo sapiens
 Database cross references - HERC2
ExoCarta ExoCarta_8924
Vesiclepedia VP_8924
Entrez Gene 8924
HGNC 4868
MIM 605837
UniProt O95714  
 HERC2 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for HERC2
Molecular Function
    ubiquitin protein ligase binding GO:0031625 IPI
    zinc ion binding GO:0008270 IEA
    SUMO binding GO:0032183 IDA
    protein binding GO:0005515 IPI
    guanyl-nucleotide exchange factor activity GO:0005085 NAS
    ligase activity GO:0016874 IEA
    ubiquitin-protein transferase activity GO:0004842 IDA
Biological Process
    cellular protein metabolic process GO:0044267 TAS
    spermatogenesis GO:0007283 IEA
    protein ubiquitination GO:0016567 IDA
    protein sumoylation GO:0016925 TAS
    cellular response to DNA damage stimulus GO:0006974 IDA
    protein ubiquitination involved in ubiquitin-dependent protein catabolic process GO:0042787 IBA
    post-translational protein modification GO:0043687 TAS
    intracellular protein transport GO:0006886 NAS
    DNA repair GO:0006281 IDA
    positive regulation of GTPase activity GO:0043547 NAS
Subcellular Localization
    membrane GO:0016020 IDA
    cytoplasm GO:0005737 IDA
    mitochondrial inner membrane GO:0005743 IEA
    nucleus GO:0005634 IDA
    centriole GO:0005814 IEA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified HERC2 in exosomes
1
Experiment ID 226
MISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH|CD9|FLOT1
EV Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
✘ EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for HERC2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MPDU1 9526
Affinity Capture-MS Homo sapiens
2 DBNL 28988
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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