Gene description for PREPL
Gene name prolyl endopeptidase-like
Gene symbol PREPL
Other names/aliases -
Species Homo sapiens
 Database cross references - PREPL
ExoCarta ExoCarta_9581
Entrez Gene 9581
HGNC 30228
MIM 609557
UniProt Q4J6C6  
 PREPL identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for PREPL
Molecular Function
    serine-type endopeptidase activity GO:0004252 IEA
    serine-type exopeptidase activity GO:0070008 IEA
Biological Process
    proteolysis GO:0006508 IEA
Subcellular Localization
    cytosol GO:0005829 IEA
    Golgi apparatus GO:0005794 IEA
    cytoskeleton GO:0005856 IEA
 Experiment description of studies that identified PREPL in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for PREPL
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which PREPL is involved
No pathways found





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