Gene description for PREPL
Gene name prolyl endopeptidase-like
Gene symbol PREPL
Other names/aliases -
Species Homo sapiens
 Database cross references - PREPL
ExoCarta ExoCarta_9581
Vesiclepedia VP_9581
Entrez Gene 9581
HGNC 30228
MIM 609557
UniProt Q4J6C6  
 PREPL identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for PREPL
Molecular Function
    serine-type endopeptidase activity GO:0004252 IEA
    peptidase activity GO:0008233 IMP
    peptidase activity GO:0008233 ISS
Biological Process
    proteolysis GO:0006508 IEA
    retrograde transport, endosome to Golgi GO:0042147 ISS
    Golgi to plasma membrane protein transport GO:0043001 ISS
    regulation of synaptic vesicle exocytosis GO:2000300 IMP
Subcellular Localization
    nucleus GO:0005634 IEA
    mitochondrion GO:0005739 HTP
    Golgi apparatus GO:0005794 IBA
    trans-Golgi network GO:0005802 ISS
    cytosol GO:0005829 IEA
    cytoskeleton GO:0005856 IBA
 Experiment description of studies that identified PREPL in exosomes
1
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for PREPL
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MRPL41 64975
Affinity Capture-MS Homo sapiens
2 C1QBP 708
Affinity Capture-MS Homo sapiens
3 TTC39B 158219
Affinity Capture-MS Homo sapiens
4 ACAA2 10449
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 PMPCA 23203
Affinity Capture-MS Homo sapiens
6 GOT2 2806
Affinity Capture-MS Homo sapiens
7 PDDC1 347862
Affinity Capture-MS Homo sapiens
8 RARS2  
Affinity Capture-MS Homo sapiens
9 PREPL 9581
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 YBEY  
Affinity Capture-MS Homo sapiens
11 C4orf26  
Affinity Capture-MS Homo sapiens
12 LIG3 3980
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 COIL  
Two-hybrid Homo sapiens
14 ARHGAP36  
Affinity Capture-MS Homo sapiens
15 SDHB 6390
Affinity Capture-MS Homo sapiens
16 APEH 327
Co-fractionation Homo sapiens
17 MRPS34 65993
Affinity Capture-MS Homo sapiens
18 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
19 UNKL  
Affinity Capture-MS Homo sapiens
20 USP22 23326
Affinity Capture-MS Homo sapiens
21 RGS4  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PREPL is involved
No pathways found





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