Gene description for MCU
Gene name mitochondrial calcium uniporter
Gene symbol MCU
Other names/aliases C10orf42
CCDC109A
Species Homo sapiens
 Database cross references - MCU
ExoCarta ExoCarta_90550
Vesiclepedia VP_90550
Entrez Gene 90550
HGNC 23526
MIM 614197
UniProt Q8NE86  
 MCU identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for MCU
Molecular Function
    calcium channel activity GO:0005262 IBA
    calcium channel activity GO:0005262 IDA
    calcium channel activity GO:0005262 IMP
    protein binding GO:0005515 IPI
    uniporter activity GO:0015292 IBA
    uniporter activity GO:0015292 IDA
    uniporter activity GO:0015292 IMP
    identical protein binding GO:0042802 IPI
Biological Process
    mitochondrial calcium ion transmembrane transport GO:0006851 IDA
    mitochondrial calcium ion transmembrane transport GO:0006851 IMP
    calcium-mediated signaling GO:0019722 IDA
    positive regulation of insulin secretion GO:0032024 IMP
    calcium import into the mitochondrion GO:0036444 IBA
    calcium import into the mitochondrion GO:0036444 IDA
    calcium import into the mitochondrion GO:0036444 IMP
    calcium import into the mitochondrion GO:0036444 NAS
    glucose homeostasis GO:0042593 IMP
    protein complex oligomerization GO:0051259 IDA
    mitochondrial calcium ion homeostasis GO:0051560 IBA
    mitochondrial calcium ion homeostasis GO:0051560 IDA
    mitochondrial calcium ion homeostasis GO:0051560 NAS
    positive regulation of mitochondrial calcium ion concentration GO:0051561 IDA
    positive regulation of mitochondrial calcium ion concentration GO:0051561 IMP
    cellular response to calcium ion starvation GO:0072732 IMP
    positive regulation of neutrophil chemotaxis GO:0090023 IMP
    positive regulation of mitochondrial fission GO:0090141 IMP
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial inner membrane GO:0005743 NAS
    mitochondrial inner membrane GO:0005743 TAS
    calcium channel complex GO:0034704 IDA
    calcium channel complex GO:0034704 ISS
    uniplex complex GO:1990246 IBA
    uniplex complex GO:1990246 IDA
    uniplex complex GO:1990246 IPI
    uniplex complex GO:1990246 ISS
    uniplex complex GO:1990246 NAS
 Experiment description of studies that identified MCU in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MCU
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MRPS27 23107
Proximity Label-MS Homo sapiens
2 AFG3L2 10939
Proximity Label-MS Homo sapiens
3 SLC1A5 6510
Co-fractionation Homo sapiens
4 VAPA 9218
Affinity Capture-MS Homo sapiens
5 CES3 23491
Affinity Capture-MS Homo sapiens
6 CMTM8  
Affinity Capture-MS Homo sapiens
7 RPA2 6118
Proximity Label-MS Homo sapiens
8 EIF2B2 8892
Affinity Capture-MS Homo sapiens
9 PDCD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 MRPL24  
Proximity Label-MS Homo sapiens
11 NDUFS7 374291
Proximity Label-MS Homo sapiens
12 GPR182  
Affinity Capture-MS Homo sapiens
13 TMEM263 90488
Proximity Label-MS Homo sapiens
14 NDUFA4 4697
Affinity Capture-MS Homo sapiens
15 MRPL49 740
Proximity Label-MS Homo sapiens
16 NDUFS2 4720
Proximity Label-MS Homo sapiens
17 SLC18A2  
Affinity Capture-MS Homo sapiens
18 SLC15A1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 NDUFS3 4722
Proximity Label-MS Homo sapiens
20 OGDH 4967
Proximity Label-MS Homo sapiens
21 IARS2 55699
Proximity Label-MS Homo sapiens
22 SDHB 6390
Proximity Label-MS Homo sapiens
23 MSH5  
Affinity Capture-MS Homo sapiens
24 KLK15  
Affinity Capture-MS Homo sapiens
25 MRPS5 64969
Proximity Label-MS Homo sapiens
26 HINT2 84681
Proximity Label-MS Homo sapiens
27 MRPS23 51649
Proximity Label-MS Homo sapiens
28 MRPS24 64951
Proximity Label-MS Homo sapiens
29 MRPL12 6182
Proximity Label-MS Homo sapiens
30 ETFA 2108
Proximity Label-MS Homo sapiens
31 MAD2L2 10459
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 MRPS26 64949
Proximity Label-MS Homo sapiens
33 Ncstn 59287
Affinity Capture-MS Mus musculus
34 NDUFS4 4724
Proximity Label-MS Homo sapiens
35 NDUFV1 4723
Proximity Label-MS Homo sapiens
36 PHB 5245
Proximity Label-MS Homo sapiens
37 NDUFV3 4731
Proximity Label-MS Homo sapiens
38 MRPS7 51081
Proximity Label-MS Homo sapiens
39 SLC30A9 10463
Proximity Label-MS Homo sapiens
40 IL13RA2 3598
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 MRPL4 51073
Proximity Label-MS Homo sapiens
42 NDUFA6  
Proximity Label-MS Homo sapiens
43 NDUFS1 4719
Proximity Label-MS Homo sapiens
44 UQCRQ 27089
Co-fractionation Homo sapiens
45 KIF14 9928
Affinity Capture-MS Homo sapiens
46 STOML2 30968
Proximity Label-MS Homo sapiens
47 NNT 23530
Proximity Label-MS Homo sapiens
48 E2F4  
Affinity Capture-MS Homo sapiens
49 KCNK1  
Affinity Capture-MS Homo sapiens
50 MRPS25 64432
Proximity Label-MS Homo sapiens
51 COLEC10 10584
Affinity Capture-MS Homo sapiens
52 MRPL45 84311
Proximity Label-MS Homo sapiens
53 ZNF526  
Affinity Capture-MS Homo sapiens
54 MRPS34 65993
Proximity Label-MS Homo sapiens
55 ACOT2 10965
Proximity Label-MS Homo sapiens
56 ATPAF1  
Proximity Label-MS Homo sapiens
57 PAM16  
Proximity Label-MS Homo sapiens
58 PTCD3 55037
Proximity Label-MS Homo sapiens
59 NDUFS8 4728
Proximity Label-MS Homo sapiens
60 MMAB 326625
Proximity Label-MS Homo sapiens
61 IL2RA  
Affinity Capture-MS Homo sapiens
62 MRPS6  
Proximity Label-MS Homo sapiens
63 CCDC109B 55013
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
64 RPL13A 23521
Proximity Label-MS Homo sapiens
65 GNAI2 2771
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 SLC25A4 291
Proximity Label-MS Homo sapiens
67 MRPS14  
Proximity Label-MS Homo sapiens
68 EIF2B4 8890
Affinity Capture-MS Homo sapiens
69 ABCB7 22
Proximity Label-MS Homo sapiens
70 COX4I1 1327
Proximity Label-MS Homo sapiens
71 MRPL41 64975
Proximity Label-MS Homo sapiens
72 NYX  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 OXA1L  
Proximity Label-MS Homo sapiens
74 MGARP  
Affinity Capture-MS Homo sapiens
75 PDE12 201626
Proximity Label-MS Homo sapiens
76 SLC22A6 9356
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 SV2A 9900
Affinity Capture-MS Homo sapiens
78 APLNR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 DAP3 7818
Proximity Label-MS Homo sapiens
80 MRPS35 60488
Proximity Label-MS Homo sapiens
81 NDUFA9 4704
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
82 LETM1 3954
Proximity Label-MS Homo sapiens
83 RTN4IP1  
Proximity Label-MS Homo sapiens
84 CMKLR1 1240
Affinity Capture-MS Homo sapiens
85 GOLT1B 51026
Affinity Capture-MS Homo sapiens
86 CRELD1 78987
Affinity Capture-MS Homo sapiens
87 P2RY2 5029
Affinity Capture-MS Homo sapiens
88 ATP5F1 515
Proximity Label-MS Homo sapiens
89 PYCR1 5831
Proximity Label-MS Homo sapiens
90 NDUFAF2  
Proximity Label-MS Homo sapiens
91 MRPL50 54534
Proximity Label-MS Homo sapiens
92 PYCR2 29920
Proximity Label-MS Homo sapiens
93 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
94 MRPS28  
Proximity Label-MS Homo sapiens
95 ARMC6 93436
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 SMDT1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 TACO1  
Proximity Label-MS Homo sapiens
98 MYC  
Affinity Capture-MS Homo sapiens
99 RPA3 6119
Proximity Label-MS Homo sapiens
100 NIN 51199
Proximity Label-MS Homo sapiens
101 SLC22A2 6582
Affinity Capture-MS Homo sapiens
102 DLST 1743
Proximity Label-MS Homo sapiens
103 ABCC1 4363
Co-fractionation Homo sapiens
104 ASPH 444
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
105 NDUFA7 4701
Proximity Label-MS Homo sapiens
106 MRPS36 92259
Proximity Label-MS Homo sapiens
107 EIF2B1 1967
Affinity Capture-MS Homo sapiens
108 COX2 4513
Proximity Label-MS Homo sapiens
109 MRPS17 51373
Proximity Label-MS Homo sapiens
110 NDUFV2 4729
Proximity Label-MS Homo sapiens
111 PHGDH 26227
Affinity Capture-MS Homo sapiens
112 IMMT 10989
Proximity Label-MS Homo sapiens
113 MAGEA8  
Affinity Capture-MS Homo sapiens
114 GLS 2744
Proximity Label-MS Homo sapiens
115 RAC1 5879
Affinity Capture-MS Homo sapiens
116 GLUD1 2746
Proximity Label-MS Homo sapiens
117 PHB2 11331
Proximity Label-MS Homo sapiens
118 SLC38A2 54407
Co-fractionation Homo sapiens
119 MRPS2 51116
Proximity Label-MS Homo sapiens
120 CCDC167  
Affinity Capture-MS Homo sapiens
121 TOR1AIP2 163590
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
122 Atp7a  
Affinity Capture-MS Mus musculus
123 ICT1 3396
Affinity Capture-MS Homo sapiens
124 NME4 4833
Proximity Label-MS Homo sapiens
125 MRPS31  
Proximity Label-MS Homo sapiens
126 MRPL43 84545
Proximity Label-MS Homo sapiens
127 COX5A 9377
Proximity Label-MS Homo sapiens
128 SLC25A40  
Proximity Label-MS Homo sapiens
129 ERLIN2 11160
Co-fractionation Homo sapiens
130 NDUFA12 55967
Proximity Label-MS Homo sapiens
131 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
132 HSD17B1  
Affinity Capture-MS Homo sapiens
133 MRPS10 55173
Proximity Label-MS Homo sapiens
134 SUCLA2 8803
Proximity Label-MS Homo sapiens
135 SHMT2 6472
Proximity Label-MS Homo sapiens
136 DDX58 23586
Affinity Capture-RNA Homo sapiens
137 EDEM2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
138 PTPMT1 114971
Proximity Label-MS Homo sapiens
139 ATAD3A 55210
Proximity Label-MS Homo sapiens
140 NDUFAF3 25915
Proximity Label-MS Homo sapiens
141 NDUFA5 4698
Proximity Label-MS Homo sapiens
142 MRPS18B 28973
Proximity Label-MS Homo sapiens
143 NDUFA2 4695
Proximity Label-MS Homo sapiens
144 SLC5A8 160728
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
145 SEC62 7095
Affinity Capture-MS Homo sapiens
146 Kif4  
Affinity Capture-MS Mus musculus
147 ACOT1 641371
Proximity Label-MS Homo sapiens
148 RAB7A 7879
Affinity Capture-MS Homo sapiens
149 FECH 2235
Proximity Label-MS Homo sapiens
150 CIT 11113
Affinity Capture-MS Homo sapiens
151 TNFRSF13C  
Affinity Capture-MS Homo sapiens
152 NDUFAF4 29078
Proximity Label-MS Homo sapiens
153 C17orf80 55028
Proximity Label-MS Homo sapiens
154 IRGC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
155 MRPS22 56945
Proximity Label-MS Homo sapiens
156 MRPL28 10573
Proximity Label-MS Homo sapiens
157 NDUFS6  
Proximity Label-MS Homo sapiens
158 MRPS9 64965
Proximity Label-MS Homo sapiens
159 TIMM44 10469
Proximity Label-MS Homo sapiens
160 ECSIT 51295
Proximity Label-MS Homo sapiens
161 SYPL1 6856
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MCU is involved
PathwayEvidenceSource
Mitochondrial calcium ion transport TAS Reactome
Processing of SMDT1 TAS Reactome
Transport of small molecules TAS Reactome





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