|
 Gene ontology annotations for SLC38A2 |
|
|
| Experiment description of studies that identified SLC38A2 in exosomes |
| 1 |
| Experiment ID |
489 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
EV Enriched markers |
|
✔
Canx
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36408942
|
| Organism |
Rattus norvegicus |
| Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
| Authors |
Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ. |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Bone marrow mesenchymal stem cells |
| Sample name |
BMSC - Passage 6 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 2 |
| Experiment ID |
490 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
EV Enriched markers |
|
✔
Canx
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36408942
|
| Organism |
Rattus norvegicus |
| Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
| Authors |
Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ. |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Bone marrow mesenchymal stem cells |
| Sample name |
BMSC - Passage 7 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 3 |
| Experiment ID |
491 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
EV Enriched markers |
|
✔
Canx
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36408942
|
| Organism |
Rattus norvegicus |
| Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
| Authors |
Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ. |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Bone marrow mesenchymal stem cells |
| Sample name |
BMSC - Passage 8 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 4 |
| Experiment ID |
492 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
|
EV Enriched markers |
|
✔
Canx
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36408942
|
| Organism |
Rattus norvegicus |
| Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
| Authors |
Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ. |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Bone marrow mesenchymal stem cells |
| Sample name |
BMSC - Passage 9 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 5 |
| Experiment ID |
426 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Exo-rich fractions 7-10 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 6 |
| Experiment ID |
426 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Exo-rich fractions 7-10 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 7 |
| Experiment ID |
427 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Breast cancer cells |
| Sample name |
MDA-MB-231 - Exo-rich fractions 1-6 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 8 |
| Experiment ID |
494 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
|
EV Enriched markers |
|
✘
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35931686
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
| Authors |
Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD. |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Chondrocytes |
| Sample name |
Osteoarthritic cartilage |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 9 |
| Experiment ID |
496 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
|
EV Enriched markers |
|
✘
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35931686
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
| Authors |
Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD. |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Chondrocytes |
| Sample name |
Healthy cartilage |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 10 |
| Experiment ID |
497 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
|
EV Enriched markers |
|
✘
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35931686
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
| Authors |
Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD. |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Chondrocytes |
| Sample name |
T/C-28a2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 11 |
| Experiment ID |
498 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
|
EV Enriched markers |
|
✘
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
35931686
|
| Organism |
Homo sapiens |
| Experiment description |
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression |
| Authors |
Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD. |
| Journal name |
Cell Death Dis
|
| Publication year |
2022 |
| Sample |
Chondrocytes |
| Sample name |
T/C-28a2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 12 |
| Experiment ID |
282 |
| MISEV standards |
|
✔
CEM
|
EV Biophysical techniques |
|
✔
Alix|TSG101|CD63|CD81|EpCAM
|
EV Enriched markers |
|
✘
|
EV Negative markers |
|
✔
DLS
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25890246
|
| Organism |
Homo sapiens |
| Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
| Authors |
Xu R, Greening DW, Rai A, Ji H, Simpson RJ. |
| Journal name |
Methods
|
| Publication year |
2015 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1863 - Ultracentrifugation - Rep 1 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 13 |
| Experiment ID |
283 |
| MISEV standards |
|
✔
CEM
|
EV Biophysical techniques |
|
✔
Alix|TSG101|CD63|CD81|EpCAM
|
EV Enriched markers |
|
✘
|
EV Negative markers |
|
✔
DLS
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25890246
|
| Organism |
Homo sapiens |
| Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
| Authors |
Xu R, Greening DW, Rai A, Ji H, Simpson RJ. |
| Journal name |
Methods
|
| Publication year |
2015 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1863 - Ultracentrifugation - Rep 2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 14 |
| Experiment ID |
286 |
| MISEV standards |
|
✔
CEM
|
EV Biophysical techniques |
|
✔
Alix|TSG101|CD63|CD81|EpCAM
|
EV Enriched markers |
|
✘
|
EV Negative markers |
|
✔
DLS
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25890246
|
| Organism |
Homo sapiens |
| Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
| Authors |
Xu R, Greening DW, Rai A, Ji H, Simpson RJ. |
| Journal name |
Methods
|
| Publication year |
2015 |
| Sample |
Colorectal cancer cells |
| Sample name |
LIM1863 - Sequential centrifugal ultrafiltration - Rep 2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Western blotting |
|
|
| 15 |
| Experiment ID |
1203 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
|
EV Enriched markers |
|
✘
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34887515
|
| Organism |
Homo sapiens |
| Experiment description |
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets |
| Authors |
Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Colorectal cancer cells |
| Sample name |
DiFi |
| Isolation/purification methods |
Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein
miRNA |
| Methods used in the study |
Western blotting
Mass spectrometry
RNA sequencing |
|
|
| 16 |
| Experiment ID |
419 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Exo-rich fractions 7-10 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 17 |
| Experiment ID |
419 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Exo-rich fractions 7-10 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 18 |
| Experiment ID |
420 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Exo-rich fractions 1-6 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 19 |
| Experiment ID |
420 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Embryonic kidney cells |
| Sample name |
HEK293T - Exo-rich fractions 1-6 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 20 |
| Experiment ID |
226 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
GAPDH|CD9|FLOT1
|
EV Enriched markers |
|
✔
LMNA|H2AFX|ATP5A1|TOMM20
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
mRNA
|
| Identification method |
Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
|
| PubMed ID |
26027894
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting |
| Authors |
Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar |
| Journal name |
Journal of Extracellular Vesicles
|
| Publication year |
2015 |
| Sample |
Endothelial cells |
| Sample name |
HMEC-1 |
| Isolation/purification methods |
Differential ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.10 g/mL
|
| Molecules identified in the study |
miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA |
| Methods used in the study |
Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting |
|
|
| 21 |
| Experiment ID |
363 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
EV Enriched markers |
|
✔
DCLK1
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
33991177
|
| Organism |
Homo sapiens |
| Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
| Authors |
Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M. |
| Journal name |
Proteomics
|
| Publication year |
2021 |
| Sample |
Gastric cancer cells |
| Sample name |
MKN1 - 100K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 22 |
| Experiment ID |
364 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
EV Enriched markers |
|
✔
DCLK1
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
33991177
|
| Organism |
Homo sapiens |
| Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
| Authors |
Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M. |
| Journal name |
Proteomics
|
| Publication year |
2021 |
| Sample |
Gastric cancer cells |
| Sample name |
MKN1 - 100K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 23 |
| Experiment ID |
365 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
|
EV Enriched markers |
|
✔
DCLK1
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
33991177
|
| Organism |
Homo sapiens |
| Experiment description |
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells |
| Authors |
Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M. |
| Journal name |
Proteomics
|
| Publication year |
2021 |
| Sample |
Gastric cancer cells |
| Sample name |
MKN1 - 100K pellet |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 24 |
| Experiment ID |
254 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|FLOT1|CD81
|
EV Enriched markers |
|
✘
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25950383
|
| Organism |
Homo sapiens |
| Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
| Authors |
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L |
| Journal name |
Pigment Cell Melanoma Res
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
MNT-1 |
| Isolation/purification methods |
Differential centrifugation
Unltracentrifugation
Sucrose density gradient |
| Flotation density |
1.13 - 1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 25 |
| Experiment ID |
255 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|FLOT1
|
EV Enriched markers |
|
✘
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25950383
|
| Organism |
Homo sapiens |
| Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
| Authors |
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L |
| Journal name |
Pigment Cell Melanoma Res
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
G1 |
| Isolation/purification methods |
Differential centrifugation
Unltracentrifugation
Sucrose density gradient |
| Flotation density |
1.13 - 1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 26 |
| Experiment ID |
256 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|FLOT1
|
EV Enriched markers |
|
✘
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25950383
|
| Organism |
Homo sapiens |
| Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
| Authors |
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L |
| Journal name |
Pigment Cell Melanoma Res
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
501mel |
| Isolation/purification methods |
Differential centrifugation
Unltracentrifugation
Sucrose density gradient |
| Flotation density |
1.13 - 1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 27 |
| Experiment ID |
257 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|FLOT1
|
EV Enriched markers |
|
✘
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25950383
|
| Organism |
Homo sapiens |
| Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
| Authors |
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L |
| Journal name |
Pigment Cell Melanoma Res
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
Daju |
| Isolation/purification methods |
Differential centrifugation
Unltracentrifugation
Sucrose density gradient |
| Flotation density |
1.13 - 1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 28 |
| Experiment ID |
258 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|FLOT1|CD81
|
EV Enriched markers |
|
✘
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25950383
|
| Organism |
Homo sapiens |
| Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
| Authors |
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L |
| Journal name |
Pigment Cell Melanoma Res
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
SKMEL28 |
| Isolation/purification methods |
Differential centrifugation
Unltracentrifugation
Sucrose density gradient |
| Flotation density |
1.13 - 1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 29 |
| Experiment ID |
259 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|FLOT1
|
EV Enriched markers |
|
✘
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25950383
|
| Organism |
Homo sapiens |
| Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
| Authors |
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L |
| Journal name |
Pigment Cell Melanoma Res
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
A375M |
| Isolation/purification methods |
Differential centrifugation
Unltracentrifugation
Sucrose density gradient |
| Flotation density |
1.13 - 1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 30 |
| Experiment ID |
260 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|FLOT1|CD81
|
EV Enriched markers |
|
✘
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25950383
|
| Organism |
Homo sapiens |
| Experiment description |
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines |
| Authors |
Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L |
| Journal name |
Pigment Cell Melanoma Res
|
| Publication year |
2015 |
| Sample |
Melanoma cells |
| Sample name |
1205Lu |
| Isolation/purification methods |
Differential centrifugation
Unltracentrifugation
Sucrose density gradient |
| Flotation density |
1.13 - 1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 31 |
| Experiment ID |
126 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
GAPDH
|
EV Enriched markers |
|
✘
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry [LTQ-FT Ultra]
|
| PubMed ID |
Unpublished / Not applicable
|
| Organism |
Homo sapiens |
| Experiment description |
Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? |
| Authors |
Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim |
| Journal name |
Mesenchymal Stem Cell Therapy
|
| Publication year |
2011 |
| Sample |
Mesenchymal stem cells |
| Sample name |
huES9.E1 |
| Isolation/purification methods |
HPLC |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Antobody array
Mass spectrometry |
|
|
| 32 |
| Experiment ID |
211 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|Alix|EpCAM|TFRC
|
EV Enriched markers |
|
✔
cytochrome c|GOLGA2
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23333927
|
| Organism |
Homo sapiens |
| Experiment description |
Characterization and proteomic analysis of ovarian cancer-derived exosomes. |
| Authors |
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. |
| Journal name |
J Proteomics
|
| Publication year |
2013 |
| Sample |
Ovarian cancer cells |
| Sample name |
IGROV1 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.09-1.15 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 33 |
| Experiment ID |
212 |
| MISEV standards |
|
✔
CEM
|
EV Biophysical techniques |
|
✔
TSG101|Alix|EpCAM|TFRC
|
EV Enriched markers |
|
✔
Cytochrome C|GOLGA2
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
23333927
|
| Organism |
Homo sapiens |
| Experiment description |
Characterization and proteomic analysis of ovarian cancer-derived exosomes. |
| Authors |
Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K. |
| Journal name |
J Proteomics
|
| Publication year |
2013 |
| Sample |
Ovarian cancer cells |
| Sample name |
OVCAR-3 |
| Isolation/purification methods |
Differential centrifugation
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.09-1.15 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 34 |
| Experiment ID |
434 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Pancreatic cancer cells |
| Sample name |
PANC-1 - Exo-rich fractions 7-10 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 35 |
| Experiment ID |
435 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
34108659
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker |
| Authors |
Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R. |
| Journal name |
Nat Cell Biol
|
| Publication year |
2021 |
| Sample |
Pancreatic cancer cells |
| Sample name |
PANC-1 - Exo-rich fractions 1-6 pooled |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 36 |
| Experiment ID |
275 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
|
EV Enriched markers |
|
✔
AIF
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25844599
|
| Organism |
Homo sapiens |
| Experiment description |
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel. |
| Authors |
Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T. |
| Journal name |
Oncotarget
|
| Publication year |
2015 |
| Sample |
Prostate cancer cells |
| Sample name |
DU145 - Docetaxel sensitive |
| Isolation/purification methods |
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.12-1.19 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry/Flow cytometry/Western blotting |
|
|
| 37 |
| Experiment ID |
274 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
|
EV Enriched markers |
|
✔
AIF
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25844599
|
| Organism |
Homo sapiens |
| Experiment description |
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel. |
| Authors |
Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T. |
| Journal name |
Oncotarget
|
| Publication year |
2015 |
| Sample |
Prostate cancer cells |
| Sample name |
DU145 - Docetaxel resistant |
| Isolation/purification methods |
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.13-1.18 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry
Flow cytometry
Western blotting |
|
|
| Protein-protein interactions for SLC38A2 |
|
|
| Pathways in which SLC38A2 is involved |
|
|
|
